FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947194

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947194
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9821172
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG122941512.518007015863281No Hit
CAACGGAATCCCAAAAGCAGCTG8366378.518708357821247No Hit
AACATTCAACGCTGTCGGTGAGT3158763.216276020825213No Hit
CCCCCACTGCTAAATTTGACTG3017513.0724540818549966No Hit
TTCAAGTAATCCAGGATAGGCT2731232.7809613760964575No Hit
TAGCAGCACGTAAATATTGGCG2643922.692061599165558No Hit
AACATTCAACGCTGTCGGTGAG2379042.422358553541268No Hit
TATTGCACTTGTCCCGGCCTGTAT2351012.393818171599072No Hit
AAGCTGCCAGTTGAAGAACTGT2290452.3321554698359828No Hit
TATTGCACTTGTCCCGGCCTGTAA2228152.2687210854264643No Hit
TAGCTTATCAGACTGATGTTGACT1660771.691009993511976No Hit
TAGCTTATCAGACTGATGTTGAC1301711.3254120791286417No Hit
TATTGCACTTGTCCCGGCCTGT1140451.161215789724485No Hit
TAGCTTATCAGACTGATGTTGA1109951.1301604329910933No Hit
TTAATGCTAATCGTGATAGGGGTT1069321.0887906249885453No Hit
TATTGCACTTGTCCCGGCCTGTA1053991.0731814899484502No Hit
TGAGGTAGTAGGTTGTATAGTT945980.9632047987755432No Hit
TGTAAACATCCTTGACTGGAAGCT660800.6728321222762416No Hit
AGCAGCATTGTACAGGGCTATGA588040.5987472778197958No Hit
TGAGGTAGTAGATTGTATAGTT570260.5806435321568546No Hit
ACTGGACTTGGAGTCAGAAGGC565330.5756237646586375No Hit
TGTAAACATCCCCGACTGGAAGCT487670.4965496989564993No Hit
CAACGGAATCCCAAAAGCAGCTGT460050.4684267824654736No Hit
TTCAAGTAATTCAGGATAGGTT428820.4366281335873153No Hit
CATAAAGTAGAAAGCACTACT407550.41497084054734No Hit
GGAATACCGGGTGCTGTAGGCTTT402190.40951324342960294No Hit
ATCCCGGACGAGCCCCCA374330.38114595691838No Hit
TCGTACGACTCTTAGCGG357090.3635920438008824No Hit
CCCCCACTGCTAAATTTGACTGGCTT351950.35835845253499277No Hit
GCCTGTCTGAGCGTCGCT346200.3525037541344353No Hit
CACTAGATTGTGAGCTCCTGGAA332440.33849320630979685No Hit
CACTAGATTGTGAGCTCCTGGA324260.33016426145474287No Hit
TATTGCACTTGTCCCGGCCTGTAG322590.3284638533975375No Hit
CGCCTGTCTGAGCGTCGCT317250.32302662044815017No Hit
CTCCCACTGCTTCACTTGACTAG315270.3210105677815234No Hit
CCCCCCACTGCTAAATTTGACTGGCT305000.310553567333919No Hit
CCCATAAAGTAGAAAGCACTAC304720.31026846897702226No Hit
CATTGCACTTGTCTCGGTCTGA304350.30989173186255164No Hit
GGAATACCGGGTGCTGTAGGCTT276920.2819622749708487No Hit
AGCTCGGTCTGAGGCCCCTCAGT276060.28108661573180876No Hit
TTCTCACTACTGCACTTGACTAG267110.27197365039528887No Hit
TATTGCACTTGTCCCGGCCTGTT266800.2716580057858675No Hit
TGAGAACTGAATTCCATGGGTT266790.2716478237016926No Hit
CACCCGGCTGTGTGCACATGTGC254620.2592562272608605No Hit
AACATTCAACGCTGTCGGTGAGTT252200.25679216289053897No Hit
CAAAGAATTCTCCTTTTGGGCT249260.2537986301431234No Hit
CCCATAAAGTAGAAAGCACT248520.25304515591418214No Hit
TGAGGTAGTAGTTTGTACAGTT243590.248025388415965No Hit
CTTCTCACTACTGCACTTGACTAG232920.2371611046013653No Hit
TCGTTTCCCGGGCGGCGCACCA229360.2335362826351071No Hit
CAACACCAGTCGATGGGCTGT222060.2261033611874428No Hit
TATTGCACTTGTCCCGGCCTGTTT207980.21176698666920812No Hit
CCCCCCACTGCTAAATTTGACTGG207610.21139024955473748No Hit
TAGCTTATCAGACTGATGTTGACA203780.2074905113157574No Hit
TGTAAACATCCTACACTCTCAGCT202220.20590210618447574No Hit
ACCCCCCACTGCTAAATTTGACTG199370.20300021219463418No Hit
GACCCGCGGGCGCTCTCCAGTCCT194230.19776662092874453No Hit
TGAGAACTGAATTCCATAGGCTGT192140.19563856533619411No Hit
TATTGCACTTGTCCCGGCCTGTAAT184000.18735034881783966No Hit
ACTGGACTTGGAGTCAGAAGGCT183700.1870448862925932No Hit
AACGGAATCCCAAAAGCAGCTG181960.18527320364616362No Hit
ACTGGACTTGGAGTCAGAAGGCA181280.1845808219222716No Hit
CTCCCACTGCTTCACTTGACTAGCCT173860.1770257154645087No Hit
ACCGGGTGCTGTAGGCTTA173670.17683225586518594No Hit
CCCCCCACTGCTAAATTTGACTGT173520.1766795246025627No Hit
TGAGGTAGTAGTTTGTGCTGTT162010.16495994571727285No Hit
TATTGCACTTGTCCCGGCCTGTAAA156160.1590034264749665No Hit
TTCAAGTAATCCAGGATAGGCTT155630.15846377601369777No Hit
TTAATGCTAATCGTGATAGGGGTTT155480.15831104475107452No Hit
TAGCAGCACGTAAATATTGGC151590.15435021400704518No Hit
TTCACAGTGGCTAAGTTCCG140660.14322119600389852No Hit
CAAAGAATTCTCCTTTTGGGCTT137460.1399629290679361No Hit
CTACGCCTGTCTGAGCGTCGCT136680.13916872650229525No Hit
TTAATGCTAATCGTGATAGGGGT134900.13735631551916613No Hit
TGAGGTAGTAGGTTGTGTGGTT132570.1349838899064185No Hit
AGCTACATCTGGCTACTGGGTCTC130190.13256055387279644No Hit
ACTGGACTTGGAGTCAGAAGG129590.1319496288223035No Hit
TTCAAGTAATCCAGGATAGGC128620.1309619666573399No Hit
AACCGGGCGGAAACACCA128390.13072777872131758No Hit
CCCCCCACTGCTAAATTTGACTGTA127610.12993357615567672No Hit
ACCGGGTGCTGTAGGCTT126910.12922083026343498No Hit
AGCAGCATTGTACAGGGCTATGT125360.12764260721632817No Hit
TTCACAGTGGCTAAGTTCTG119170.12133989711207584No Hit
ACCGGGTGCTGTAGGCTTT118160.12031150661041268No Hit
TGTAAACATCCCCGACTGGAAGC115240.11733833803134698No Hit
CAACGGAATCCCAAAAGCAGCT114260.11634049378220848No Hit
TGAGGGGCAGAGAGCGAGACTTT113240.11530192119637045No Hit
CCCCCCACTGCTAAATTTGACTGA113240.11530192119637045No Hit
TACGCCTGTCTGAGCGTCGCT112890.11494554825024957No Hit
TATTGCACTTGTCCCGGCCTGA110150.11215565718633172No Hit
TATTGCACTTGTCCCGGCCTG109970.11197237967118384No Hit
ACCACAGGGTAGAACCACGGAC108700.11067925498097375No Hit
TGTCAGTTTGTCAAATACCCCAA100620.10245213096766863No Hit
CCCCCACAACCGCGCTTGACTAG99270.10107754960405947No Hit
TTCACCACCTTCTCCACCCAGC98810.10060917373201488No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)