FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947196

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947196
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5586089
Sequences flagged as poor quality0
Sequence length18-26
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG4993638.939402863076475No Hit
AACATTCAACGCTGTCGGTGAGT2817085.043027420436731No Hit
AACATTCAACGCTGTCGGTGAG2135053.8220837512613923No Hit
TTCAAGTAATCCAGGATAGGCT1906923.413694268029027No Hit
CCCCCCACTGCTAAATTTGACTG1846183.30495987443093No Hit
TAGCTTATCAGACTGATGTTGACT1753393.13885081315389No Hit
TAGCAGCACGTAAATATTGGCG1564952.801512829459037No Hit
TAGCTTATCAGACTGATGTTGAC1563392.7987201779277058No Hit
TATTGCACTTGTCCCGGCCTGTAA1397302.5013922979028798No Hit
TATTGCACTTGTCCCGGCCTGTAT1231542.2046551710866047No Hit
AAGCTGCCAGTTGAAGAACTGT1213142.1717162043068057No Hit
TAGCTTATCAGACTGATGTTGA1123442.0111387412552864No Hit
TGAGGTAGTAGGTTGTATAGTT941411.6852756910962212No Hit
GCCTGTCTGAGCGTCGCT690621.2363211542100385No Hit
CCCCCACTGCTAAATTTGACTG634221.1353560603850028No Hit
TGAGGTAGTAGATTGTATAGTT570121.0206067250271165No Hit
TATTGCACTTGTCCCGGCCTGT537390.9620147477063112No Hit
TGTAAACATCCTTGACTGGAAGCT520220.9312776792492924No Hit
TATTGCACTTGTCCCGGCCTGTA476650.8532803541082141No Hit
AGCAGCATTGTACAGGGCTATGA422340.7560566972706665No Hit
TGTAAACATCCCCGACTGGAAGCT410540.7349327946618824No Hit
TTCAAGTAATTCAGGATAGGTT387400.6935084636138092No Hit
CATAAAGTAGAAAGCACTACT384050.6875114234664002No Hit
TCCTGTCTGAGCGTCGCT296430.5306574957899883No Hit
ACTGGACTTGGAGTCAGAAGGC274090.4906652937323412No Hit
CAACGGAATCCCAAAAGCAGCT265800.47582485706905137No Hit
CAACGGAATCCCAAAAGCAGCTGT262690.4702574556187701No Hit
CATTGCACTTGTCTCGGTCTGA259840.4651554960903774No Hit
CAACACCAGTCGATGGGCTGT246330.44097041776455764No Hit
TAGCTTATCAGACTGATGTTGACA242610.43431101795907656No Hit
TGATGTAGTAGGTTGTATAGTT208540.37332022457930764No Hit
TGAGGTAGTAGTTTGTACAGTT206060.3688806247089869No Hit
TGTCAGTTTGTCAAATACCCCAA205150.36725157798237734No Hit
CCCATAAAGTAGAAAGCACTAC201530.3607711943006994No Hit
TCGTACGACTCTTAGCGG196490.3517487816610154No Hit
CAAAGAATTCTCCTTTTGGGCT192020.34374676092700995No Hit
CACTAGATTGTGAGCTCCTGGAA185370.3318421886940935No Hit
CACTAGATTGTGAGCTCCTGGA184420.33014153551796255No Hit
TCGTTTCCCGGCCAATGCACCA179620.32154876157540635No Hit
AACATTCAACGCTGTCGGTGAGTT172220.3083015684139655No Hit
CCCATAAAGTAGAAAGCACT164820.2950543752525246No Hit
TATTGCACTTGTCCCGGCCTGTAG160480.28728507547946336No Hit
TATTGCACTTGTCCCGGCCTGTAAT144210.2581591521366738No Hit
CAAAGAATTCTCCTTTTGGGCTT134290.2404007526553909No Hit
CCCCCACTGCTAAATTTGACTGGCTT131570.23553151408794237No Hit
TGAGGTAGTAGTTTGTGCTGTT128470.22998201425004147No Hit
TGATGTAGTAGATTGTATAGTT125400.22448621924928158No Hit
TAGCAGCACGTAAATATTGGC119510.21394216955726986No Hit
TATTGCACTTGTCCCGGCCTGTAAA119090.21319030183729618No Hit
TGTAAACATCCTACACTCTCAGCT116210.20803463747176243No Hit
TTCAAGTAATCCAGGATAGGCTT114720.20536729722709393No Hit
TTCTCACTACTGCACTTGACTAG114430.20484815046806448No Hit
CTTCTCACTACTGCACTTGACTAG113810.20373825050048433No Hit
AACGGAATCCCAAAAGCAGCTG112660.2016795650767469No Hit
TTATAAAGCAATGAGACTGATT110310.1974726861673704No Hit
TGTCAGTTTGTCAAATACCCCA108190.1936775443427414No Hit
TATTGCACTTGTCCCGGCCTGTT103170.18469093492781802No Hit
AACATTCAACGCTGTCGGTGA99450.17803153512233694No Hit
TGTAAACATCCCCGACTGGAAGC95640.1712110208054329No Hit
TTCACAGTGGCTAAGTTCCGC93050.1665745031989286No Hit
CACCCGGCTGTGTGCACATGTGC92590.16575102902943367No Hit
AGCAGCATTGTACAGGGCTATGT88140.1577848115201888No Hit
TGAGAACTGAATTCCATAGGCTGT88030.15758789378400523No Hit
AACATTCAACGCTGTCGGTGAGA83470.1494247585385768No Hit
ACTGGACTTGGAGTCAGAAGGCA82330.1473839747272197No Hit
TCAGTGCACTACAGAACTTTGT82270.14727656505293776No Hit
TGAGGTAGTAGGTTGTATAGT80210.1435888329025907No Hit
TTCAAGTAATCCAGGATAGGC78520.1405634604103157No Hit
ATGCACCTGGGCAAGGATTCTGA77760.13920293786941096No Hit
TATTGCACTTGTCCCGGCCTGTTT77730.13914923303226998No Hit
GGAATACCGGGTGCTGTAGGCTTT77110.1380393330646898No Hit
ACTGGACTTGGAGTCAGAAGGCT76390.13675041697330637No Hit
TTCACAGTGGCTAAGTTCTGC72090.12905272364976642No Hit
CAACGGAATCCCAAAAGCAGCTGA70750.1266539075908028No Hit
CGCCTGTCTGAGCGTCGCT70650.12647489146699953No Hit
TTCACAGTGGCTAAGTTCTG70530.12626007211843565No Hit
ACTTGACTTGGAGTCAGAAGGC67220.1203346384205479No Hit
CCCCCCACTGCTAAATTTGACTGGCT66510.1190636239415448No Hit
CTCCCACTGCTTCACTTGACTAG65850.11788211752444332No Hit
CCCATAAAGTAGAAAGCACTA65360.11700493851780736No Hit
TAGCTTATCAGACTGATGTTGACC63780.11417648376171594No Hit
ATCCCGGACGAGCCCCCA62890.11258324025986696No Hit
TGAGGTAGTAAGTTGTATTGTT62530.11193878221417526No Hit
CAAAGTGCTGTTCGTGCAGGTAG60690.10864488553619536No Hit
CAAGGGAATCCCAAAAGCAGCTG60310.10796462426574298No Hit
CCCCCCACTGCTAAATTTGACTGG58780.10522567757155318No Hit
AGCAGCATTGTACAGGGCTATG58320.10440220340205823No Hit
ATCACATTGCCAGGGATTTCC57860.10357872923256325No Hit
CTCCCACTGCTTCACTTGACTAGCCT57540.10300587763639284No Hit
ACTGGACTTGGAGTCAGAAGG57530.1029879760240125No Hit
TCAGTGCACTACAGAACTTTGTC57140.10228981314117981No Hit
TGAGGTAGTAGGTTGTGTGGTT57130.1022719115287995No Hit
ATCACATTGCCAGGGATTTCCA57030.10209289540499622No Hit
ACCACAGGGTAGAACCACGGAC56210.10062496318980954No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)