FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947197

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947197
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9046037
Sequences flagged as poor quality0
Sequence length18-26
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG6215376.870820890960318No Hit
AACATTCAACGCTGTCGGTGAGT4745105.2455014278628305No Hit
TTCAAGTAATCCAGGATAGGCT3311383.660586398220569No Hit
AAGCTGCCAGTTGAAGAACTGT3057883.380353186704852No Hit
TAGCTTATCAGACTGATGTTGACT2870543.1732569742971424No Hit
AACATTCAACGCTGTCGGTGAG2863933.1659499071250754No Hit
TAGCAGCACGTAAATATTGGCG2856363.1575816017555534No Hit
TAGCTTATCAGACTGATGTTGAC2779713.0728483644274283No Hit
CCCCCCACTGCTAAATTTGACTG2147742.3742330481292524No Hit
TAGCTTATCAGACTGATGTTGA1593871.7619538810199429No Hit
TATTGCACTTGTCCCGGCCTGTAA1586491.7537956123770002No Hit
TATTGCACTTGTCCCGGCCTGTAT1362611.5063060210786228No Hit
TGTAAACATCCTTGACTGGAAGCT1269871.4037859893785534No Hit
TGAGGTAGTAGGTTGTATAGTT1135041.2547372954587739No Hit
GGAATACCGGGTGCTGTAGGCTTT813740.8995541362477292No Hit
CATAAAGTAGAAAGCACTACT765850.846613826585056No Hit
AGCAGCATTGTACAGGGCTATGA732650.8099126722563703No Hit
CCCCCACTGCTAAATTTGACTG708920.7836801905630056No Hit
TGAGGTAGTAGATTGTATAGTT691760.7647105577834802No Hit
TATTGCACTTGTCCCGGCCTGT686670.7590837844240522No Hit
TGTAAACATCCCCGACTGGAAGCT673960.7450334328723175No Hit
TATTGCACTTGTCCCGGCCTGTA592030.6544633854581846No Hit
TTCAAGTAATTCAGGATAGGTT543940.6013019845043747No Hit
GGAATACCGGGTGCTGTAGGCTT519760.5745720474059525No Hit
GCCTGTCTGAGCGTCGCT496570.5489365121986567No Hit
CCCATAAAGTAGAAAGCACTAC488980.5405460977000205No Hit
TGAATACCGGGTGCTGTAGGCTTT469920.5194760976546967No Hit
ACTGGACTTGGAGTCAGAAGGC433880.47963544699187055No Hit
TTAATGCTAATCGTGATAGGGGTT427650.4727484532729636No Hit
CGCCTGTCTGAGCGTCGCT401220.443531239149254No Hit
TAGCTTATCAGACTGATGTTGACA383900.4243847333368192No Hit
CAACGGAATCCCAAAAGCAGCTGT368630.40750441325853526No Hit
AACATTCAACGCTGTCGGTGAGTT343010.379182618863929No Hit
TGAGGTAGTAGTTTGTACAGTT316710.3501091140794582No Hit
TGTCAGTTTGTCAAATACCCCAA307340.3397509870897057No Hit
TGTAAACATCCTACACTCTCAGCT300300.33196857364169524No Hit
TGAATACCGGGTGCTGTAGGCTT297240.32858587688730434No Hit
CCCATAAAGTAGAAAGCACT252450.2790724822372493No Hit
CAAAGAATTCTCCTTTTGGGCT251320.27782331644232716No Hit
CATTGCACTTGTCTCGGTCTGA244420.2701956668981124No Hit
TGATGTAGTAGGTTGTATAGTT242330.2678852629057343No Hit
CAACACCAGTCGATGGGCTGT237610.2626675084349091No Hit
TCCTGTCTGAGCGTCGCT237240.2622584895463063No Hit
TTCAAGTAATCCAGGATAGGCTT231360.2557584055868885No Hit
TTCTCACTACTGCACTTGACTAG218700.2417633268579379No Hit
CAAAGAATTCTCCTTTTGGGCTT205400.22706075599734998No Hit
TAGCAGCACGTAAATATTGGC192020.21226974862030745No Hit
CCCCCACTGCTAAATTTGACTGGCTT190450.21053418198488466No Hit
TGTCAGTTTGTCAAATACCCCA185420.20497373601279764No Hit
CTTCTCACTACTGCACTTGACTAG185320.20486319036722933No Hit
TTATAAAGCAATGAGACTGATT183810.20319395111914754No Hit
TATTGCACTTGTCCCGGCCTGTAG179130.19802041490654967No Hit
AACCGGGCGGAAACACCA173380.1916640402863707No Hit
TGTAAACATCCCCGACTGGAAGC165290.18272089755989282No Hit
TCGTACGACTCTTAGCGG163540.180786348762447No Hit
TGAGGTAGTAGTTTGTGCTGTT161310.17832118086627327No Hit
AGCAGCATTGTACAGGGCTATGT159330.17613237708402033No Hit
AACATTCAACGCTGTCGGTGAGA157430.17403200981822206No Hit
AACATTCAACGCTGTCGGTGA156480.1729818261853229No Hit
TTTAAACATCCTTGACTGGAAGCT155870.1723074977473561No Hit
TACGCCTGTCTGAGCGTCGCT155100.17145629627647999No Hit
TGATGTAGTAGATTGTATAGTT146740.16221468030696756No Hit
TTCACAGTGGCTAAGTTCCG143880.15905307484371334No Hit
CTACGCCTGTCTGAGCGTCGCT138810.15344841061339898No Hit
TTCACAGTGGCTAAGTTCCGC137660.1521771356893632No Hit
TATTGCACTTGTCCCGGCCTGTAAT136430.15081742424887273No Hit
TATTGCACTTGTCCCGGCCTGTT135650.14995516821343977No Hit
TGTAAACATCCTACACTCAGCT134240.1483964746109263No Hit
AACGGAATCCCAAAAGCAGCTG134030.14816432875523283No Hit
TCGTTTCCCGGCCAATGCACCA133490.14756738226916383No Hit
CAACGGAATCCCAAAAGCAGCT132470.14643981668436687No Hit
CACTAGATTGTGAGCTCCTGGAA132070.14599763410209354No Hit
TGCAAGTAATCCAGGATAGGCT129660.14333348404389679No Hit
TTCAAGTAATCCAGGATAGGC128800.14238279149200916No Hit
GGAATACCGGGTGCTGTAGGCTTA128180.1416974084894855No Hit
AGCTCGGTCTGAGGCCCCTCAGT126620.13997289641861954No Hit
TAGCAGCACGTAAATATTGGCGT126530.13987340533760806No Hit
ATCACATTGCCAGGGATTTCCA124800.13796096566927596No Hit
ACTGGACTTGGAGTCAGAAGGCT122230.13511994257816987No Hit
CACTAGATTGTGAGCTCCTGGA121150.1339260496060319No Hit
ACTGGACTTGGAGTCAGAAGGCA120730.1334617578946449No Hit
TTAGGTAGTAGGTTGTATAGTT117910.13034437068961802No Hit
ATCACATTGCCAGGGATTTCC116120.1283656036339449No Hit
CCCCCCACTGCTAAATTTGACTGGCT114400.12646421853016962No Hit
CTCCCACTGCTTCACTTGACTAG114040.12606625420612363No Hit
TATTGCACTTGTCCCGGCCTGTAAA113790.12578989009220282No Hit
TGAGAACTGAATTCCATAGGCTGT110150.1217660285935156No Hit
ACCACAGGGTAGAACCACGGAC106940.11821751337077219No Hit
TGAGAACTGAATTCCATGGGTT106600.11784165817583987No Hit
TTCACAGTGGCTAAGTTCTGC103670.11460267076068781No Hit
TAGCAGCACATCATGGTTTACA103110.11398361514550516No Hit
CAAAGTGCTGTTCGTGCAGGTAG100820.1114521198619904No Hit
TATTGCACTTGTCCCGGCCTGTTT100290.11086622794047823No Hit
TGAGGTAGTAGGTTGTATAGT99780.11030244514807976No Hit
ACTTGACTTGGAGTCAGAAGGC99090.10953968019365828No Hit
TTCACAGTGGCTAAGTTCTG97180.10742825836330319No Hit
ACTGGACTTGGAGTCAGAAGG96150.10628963821394939No Hit
ACGCCTGTCTGAGCGTCGCT94880.10488570851523157No Hit
TGTAAACATCCTTGACTGGAAGC93350.10319436013803614No Hit
AGCAGCATTGTACAGGGCTATG92140.10185675782665934No Hit
CCCCCCACTGCTAAATTTGACTGG91360.10099450179122636No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)