FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947198

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947198
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7596961
Sequences flagged as poor quality0
Sequence length18-26
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG6709878.832308076874423No Hit
TAGCAGCACGTAAATATTGGCG3685434.851189837620596No Hit
TTCAAGTAATCCAGGATAGGCT3206754.221095777640559No Hit
AAGCTGCCAGTTGAAGAACTGT3092434.070614552319013No Hit
AACATTCAACGCTGTCGGTGAGT2626063.456724340167075No Hit
TAGCTTATCAGACTGATGTTGAC2377783.1299094466853257No Hit
TAGCTTATCAGACTGATGTTGACT1764372.3224681553584388No Hit
CCCCCCACTGCTAAATTTGACTG1709472.2502024164662684No Hit
TAGCTTATCAGACTGATGTTGA1630012.14560796086751No Hit
AACATTCAACGCTGTCGGTGAG1415841.8636926002384375No Hit
TATTGCACTTGTCCCGGCCTGTAA1234711.6252683145273485No Hit
TATTGCACTTGTCCCGGCCTGTAT1171171.5416296068914925No Hit
TGTAAACATCCTTGACTGGAAGCT992081.3058906054671071No Hit
TGAGGTAGTAGGTTGTATAGTT881531.1603718908126552No Hit
AGCAGCATTGTACAGGGCTATGA744840.9804446804452465No Hit
TTAATGCTAATCGTGATAGGGGTT732520.9642276694588796No Hit
TATTGCACTTGTCCCGGCCTGT642160.8452853713478323No Hit
CATAAAGTAGAAAGCACTACT641410.8442981344777207No Hit
TTCAAGTAATTCAGGATAGGTT640950.8436926291973855No Hit
TGAGGTAGTAGATTGTATAGTT552250.7269354153588521No Hit
TGTAAACATCCCCGACTGGAAGCT506980.667345797878915No Hit
CCCATAAAGTAGAAAGCACTAC495700.6524977553524363No Hit
TATTGCACTTGTCCCGGCCTGTA489350.644139149852158No Hit
CCCCCACTGCTAAATTTGACTG486030.6397689813071306No Hit
CAACGGAATCCCAAAAGCAGCTGT481100.6332795442809303No Hit
TGTAAACATCCTACACTCTCAGCT454310.5980154432805433No Hit
GCCTGTCTGAGCGTCGCT379150.4990811457370914No Hit
ACTGGACTTGGAGTCAGAAGGC337090.4437169020612321No Hit
CAAAGAATTCTCCTTTTGGGCT334530.4403471335445845No Hit
TAGCTTATCAGACTGATGTTGACA327120.4305932332678817No Hit
CAACGGAATCCCAAAAGCAGCT269540.3547997679598461No Hit
CATTGCACTTGTCTCGGTCTGA263350.3466517729918582No Hit
TGATGTAGTAGGTTGTATAGTT251190.3306453725377819No Hit
CAAAGAATTCTCCTTTTGGGCTT235750.3103214561717508No Hit
CCCCCACTGCTAAATTTGACTGGCTT219890.28944468715845717No Hit
AACATTCAACGCTGTCGGTGAGTT216730.28528512914572024No Hit
TGAGGTAGTAGTTTGTACAGTT209700.2760314288832074No Hit
GGAATACCGGGTGCTGTAGGCTTT205330.2702791287200237No Hit
TTCAAGTAATCCAGGATAGGCTT198540.2613413442559465No Hit
CACTAGATTGTGAGCTCCTGGA196950.25924840209130995No Hit
TTCTCACTACTGCACTTGACTAG194310.255773328308517No Hit
CTTCTCACTACTGCACTTGACTAG188810.2485335912610319No Hit
TAGCAGCACGTAAATATTGGC184340.24264965951516665No Hit
CACTAGATTGTGAGCTCCTGGAA181320.23867438571818386No Hit
TCGTTTCCCGGCCAATGCACCA178880.23546257510075408No Hit
CCCATAAAGTAGAAAGCACT176810.23273780133924604No Hit
TAGCAGCACGTAAATATTGGCGT175330.2307896539155591No Hit
AGCTCGGTCTGAGGCCCCTCAGT174340.22948650124701178No Hit
TGAGGTAGTAGTTTGTGCTGTT173740.2286967117509225No Hit
TGTAAACATCCTACACTCAGCT160540.21132134283695808No Hit
TGTAAACATCCCCGACTGGAAGC157160.20687219534232174No Hit
TTCACAGTGGCTAAGTTCCG156090.2054637374076292No Hit
TGTCAGTTTGTCAAATACCCCA155850.20514782160919345No Hit
TGATGTAGTAGATTGTATAGTT154880.20387099525718244No Hit
AGCAGCATTGTACAGGGCTATGT153050.2014621372941101No Hit
TTCAAGTAATCCAGGATAGGC150010.19746053718059103No Hit
GGAATACCGGGTGCTGTAGGCTT146580.19294557389461392No Hit
TATTGCACTTGTCCCGGCCTGTAG144700.1904709001402008No Hit
TCCTGTCTGAGCGTCGCT141360.18607440527863706No Hit
ATCACATTGCCAGGGATTTCCA139580.1837313631069055No Hit
CCCCCCACTGCTAAATTTGACTGGCT137360.18080914197137513No Hit
TATTGCACTTGTCCCGGCCTGTT134550.1771102944980236No Hit
CTACGCCTGTCTGAGCGTCGCT132400.17428021547037034No Hit
TTAATGCTAATCGTGATAGGGGTTT132360.1742275628372977No Hit
TGTCAGTTTGTCAAATACCCCAA130910.17231890488841525No Hit
TTATAAAGCAATGAGACTGATT124660.16409193097081848No Hit
TGAGAACTGAATTCCATAGGCTGT120420.1585107518651208No Hit
TTCACAGTGGCTAAGTTCCGC117840.15511465703193686No Hit
CGCCTGTCTGAGCGTCGCT116980.15398262542087554No Hit
AACGGAATCCCAAAAGCAGCTG116210.1529690622342276No Hit
ATCACATTGCCAGGGATTTCC110980.14608473045998263No Hit
TATTGCACTTGTCCCGGCCTGTTT107600.14163558296534626No Hit
CCCATAAAGTAGAAAGCACTA107440.1414249724330558No Hit
TGTCAGTTTGTCAAATACCCC106780.14055620398735758No Hit
CTCCCACTGCTTCACTTGACTAG104490.13754184074395012No Hit
TATTGCACTTGTCCCGGCCTGTAAT103600.13637031965808433No Hit
CAACACCAGTCGATGGGCTGT103190.13583063016908997No Hit
TTAATGCTAATCGTGATAGGGGT102580.13502767751473252No Hit
CAAAGTGCTGTTCGTGCAGGTAG101250.13327697746506795No Hit
AACATTCAACGCTGTCGGTGAGA101080.1330532037745093No Hit
TTCACAGTGGCTAAGTTCTGC100480.13226341427842No Hit
AGCAGCATTGTACAGGGCTATG96960.12762998256802952No Hit
AACCGGGCGGAAACACCA96750.12735355624439826No Hit
TGTAGTGTTTCCTACTTTATGGA96410.126906008863281No Hit
ACTTGACTTGGAGTCAGAAGGC95120.125207961446689No Hit
ACTGGACTTGGAGTCAGAAGGCA93850.12353624034663335No Hit
TCAGTGCACTACAGAACTTTGT92920.12231206662769495No Hit
TAGCAGCACATCATGGTTTACA92650.12195666135445477No Hit
TGTAAACATCCTTGACTGGAAGC91730.12074565079378452No Hit
AGCTACATCTGGCTACTGGGTCTC91010.11979790339847736No Hit
CCCCCCACTGCTAAATTTGACTGG90270.11882382968663391No Hit
TATTGCACTTGTCCCGGCCTGTAAA88240.11615170855819848No Hit
TGAGAACTGAATTCCATGGGTT87050.11458529272428805No Hit
TCGTTTCCCGGGCGGCGCACCA86860.1143351927171931No Hit
TGAATACCGGGTGCTGTAGGCTTT85690.11279510319981897No Hit
GGGCTACGCCTGTCTGAGCGTCGCT84580.11133399263205379No Hit
ATGCACCTGGGCAAGGATTCTGA82340.10838544517998709No Hit
CAGTGCAATAGTATTGTCAAAGC82220.10822748728076925No Hit
CAACGGAATCCCAAAAGCAGCTGA80430.10587128195076953No Hit
AACATTCAACGCTGTCGGTGA80020.10533159246177518No Hit
TCGTACGACTCTTAGCGG79310.10439700822473619No Hit
GTCAGGATGGCCGAGCGGTCTAAGG77580.10211978184434538No Hit
CAAAGTGCTGTTCGTGCAGGT76410.10057969232697127No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)