FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947199

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947199
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7714486
Sequences flagged as poor quality0
Sequence length18-26
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG4805566.229267899377872No Hit
AACATTCAACGCTGTCGGTGAGT3639994.718383052351123No Hit
TAGCAGCACGTAAATATTGGCG3086594.001031306557559No Hit
TTCAAGTAATCCAGGATAGGCT3027493.9244221844462484No Hit
AAGCTGCCAGTTGAAGAACTGT2853763.6992224757423893No Hit
TAGCTTATCAGACTGATGTTGAC2251772.918885328199442No Hit
AACATTCAACGCTGTCGGTGAG2009632.605008292192118No Hit
TAGCTTATCAGACTGATGTTGACT1966922.5496449147746203No Hit
CCCCCCACTGCTAAATTTGACTG1800942.33449124154221No Hit
TAGCTTATCAGACTGATGTTGA1747512.2652319286080758No Hit
TGAGGTAGTAGGTTGTATAGTT1038011.3455335844798992No Hit
CATAAAGTAGAAAGCACTACT963611.2490916439539848No Hit
TATTGCACTTGTCCCGGCCTGTAA931141.2070019959852154No Hit
TGTAAACATCCTTGACTGGAAGCT905831.1741935885294237No Hit
TATTGCACTTGTCCCGGCCTGTAT829371.075081346962066No Hit
TATTGCACTTGTCCCGGCCTGT702410.910507842000102No Hit
CCCCCACTGCTAAATTTGACTG671750.8707644294124067No Hit
AGCAGCATTGTACAGGGCTATGA640790.8306321380322681No Hit
CGCCTGTCTGAGCGTCGCT603100.781775999074987No Hit
TGAGGTAGTAGATTGTATAGTT574720.74498806530986No Hit
TTCAAGTAATTCAGGATAGGTT532180.6898450525414136No Hit
TGTAAACATCCCCGACTGGAAGCT497460.6448388136293202No Hit
TATTGCACTTGTCCCGGCCTGTA492190.6380075095087345No Hit
CCCATAAAGTAGAAAGCACTAC424650.5504579307033547No Hit
ACTGGACTTGGAGTCAGAAGGC396500.5139681373457674No Hit
TCGTACGACTCTTAGCGG322020.4174224958085348No Hit
TGTAAACATCCTACACTCTCAGCT308030.39928778145426663No Hit
AACATTCAACGCTGTCGGTGAGTT304130.39423235715250504No Hit
CATTGCACTTGTCTCGGTCTGA298360.38675292171118075No Hit
CAACGGAATCCCAAAAGCAGCTGT288180.37355696802094135No Hit
CCCATAAAGTAGAAAGCACT284410.3686700578625718No Hit
CAACACCAGTCGATGGGCTGT270040.35004276370454235No Hit
TTCACAGTGGCTAAGTTCCG266900.34597249901030347No Hit
TAGCTTATCAGACTGATGTTGACA255120.33070252509370035No Hit
TGAGGTAGTAGTTTGTACAGTT248650.3223157058033419No Hit
TGTCAGTTTGTCAAATACCCCAA241360.3128659511469721No Hit
TGATGTAGTAGGTTGTATAGTT223650.2899091397664083No Hit
CAAAGAATTCTCCTTTTGGGCT220480.2857999871929251No Hit
TTATAAAGCAATGAGACTGATT194760.25246011205412777No Hit
TTCTCACTACTGCACTTGACTAG194740.25243418680129825No Hit
CTACGCCTGTCTGAGCGTCGCT190680.24717136047690022No Hit
TACGCCTGTCTGAGCGTCGCT187990.24368441397132615No Hit
TTCAAGTAATCCAGGATAGGCTT182720.23685310985074054No Hit
CTTCTCACTACTGCACTTGACTAG165280.21424628938337564No Hit
CAAAGAATTCTCCTTTTGGGCTT164860.21370185907395514No Hit
TGAGGTAGTAGTTTGTGCTGTT161200.20895753780614806No Hit
TAGCAGCACGTAAATATTGGC157760.204498394319466No Hit
ATCCCGGACGAGCCCCCA156110.20235956096102836No Hit
CAACGGAATCCCAAAAGCAGCT153750.19930038112714188No Hit
GGAATACCGGGTGCTGTAGGCTT153730.19927445587431228No Hit
TGTAAACATCCTACACTCAGCT152200.19729117403285198No Hit
TTCAAGTAATCCAGGATAGGC150540.19513937804799958No Hit
TGTCAGTTTGTCAAATACCCCA149230.19344127398766425No Hit
CCCCCACTGCTAAATTTGACTGGCTT146720.1901876547575561No Hit
GGAATACCGGGTGCTGTAGGCTTT145430.18851547595005033No Hit
AGCAGCATTGTACAGGGCTATGT144530.18734883957272072No Hit
ACGCCTGTCTGAGCGTCGCT144260.18699884865952185No Hit
CACTAGATTGTGAGCTCCTGGA137830.1786638798748225No Hit
TGTAAACATCCCCGACTGGAAGC136850.1773935424861747No Hit
TAGCAGCACGTAAATATTGGCGT134180.1739325212334302No Hit
AACATTCAACGCTGTCGGTGA133860.17351771718815745No Hit
TATTGCACTTGTCCCGGCCTGTT130410.1690456110750606No Hit
ACCGGGTGCTGTAGGCTT129560.16794378782980487No Hit
TCGTTTCCCGGCCAATGCACCA126840.16441795344498647No Hit
AACATTCAACGCTGTCGGTGAGA125770.16303095241860574No Hit
TGATGTAGTAGATTGTATAGTT125470.1626420736261625No Hit
CACTAGATTGTGAGCTCCTGGAA122240.1584551452941907No Hit
AGCAGCATTGTACAGGGCTATG119050.15432006746787796No Hit
TTCACAGTGGCTAAGTTCTG117000.15166272905284942No Hit
GCGTTCGCGCTTTCCCCTG116170.1505868310604232No Hit
TTCACAGTGGCTAAGTTCCGC111610.1446758734152865No Hit
AACGGAATCCCAAAAGCAGCTG110740.14354812491720123No Hit
ACTGGACTTGGAGTCAGAAGG107810.1397500753776726No Hit
TATTGCACTTGTCCCGGCCTGTAG107410.13923157032108166No Hit
ACCACAGGGTAGAACCACGGAC104920.13600387634380307No Hit
ACTGGACTTGGAGTCAGAAGGCA104020.13483723996647348No Hit
TGAGGTAGTAGGTTGTATAGT103080.13361875308348475No Hit
CCCCCCACTGCTAAATTTGACTGGCT102250.13254285509105856No Hit
CAAAGTGCTGTTCGTGCAGGT100950.130857713657138No Hit
CTCCCACTGCTTCACTTGACTAG100480.13024847021564365No Hit
CCCATAAAGTAGAAAGCACTA93660.12140795900076817No Hit
ATCACATTGCCAGGGATTTCCA93550.12126537011020566No Hit
CAAAGTGCTGTTCGTGCAGGTAG93150.12074686505361473No Hit
ACTTGACTTGGAGTCAGAAGGC91610.11875062058573961No Hit
AGCTCGGTCTGAGGCCCCTCAGT89960.11661178722730198No Hit
TTCACAGTGGCTAAGTTCTGC89470.11597661853297808No Hit
TGAGGTAGTAGGTTGTGTGGTT87570.11351371951417114No Hit
ACTGGACTTGGAGTCAGAAGGCT85480.11080453059348347No Hit
ATCACATTGCCAGGGATTTCC83640.10841940733316516No Hit
CCCCCCACTGCTAAATTTGACTGG83320.10800460328789241No Hit
CCAAGTCCTTCTGATCGAGGCCC82730.10723980832942077No Hit
TAGCTTATCAGACTGATGTTG81690.10589169518228435No Hit
TCAGTGCACTACAGAACTTTGT80540.10440099314458538No Hit
ACCGGGTGCTGTAGGCTTT79270.10275473958990917No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)