FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947200

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947200
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10235195
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG137398313.424101836848248No Hit
CAACGGAATCCCAAAAGCAGCTG8951758.746047339596362No Hit
AACATTCAACGCTGTCGGTGAGT5170545.051725931943651No Hit
AAGCTGCCAGTTGAAGAACTGT3856613.767988787707513No Hit
AACATTCAACGCTGTCGGTGAG3438803.359779662234085No Hit
TAGCAGCACGTAAATATTGGCG3057032.9867823720017057No Hit
TTCAAGTAATCCAGGATAGGCT2684342.622656432046483No Hit
TATTGCACTTGTCCCGGCCTGTAA2377102.3224765136375027No Hit
CCCCCACTGCTAAATTTGACTG2304702.2517401964496035No Hit
TATTGCACTTGTCCCGGCCTGTAT2190602.140262105411768No Hit
TAGCTTATCAGACTGATGTTGACT1662111.623916300568773No Hit
TAGCTTATCAGACTGATGTTGAC1338321.3075666853440506No Hit
TGAGGTAGTAGGTTGTATAGTT1279191.2497954362374142No Hit
TATTGCACTTGTCCCGGCCTGT1251701.2229371301670364No Hit
TAGCTTATCAGACTGATGTTGA1190071.162723328671315No Hit
TATTGCACTTGTCCCGGCCTGTA932200.9107789348419839No Hit
TGAGGTAGTAGATTGTATAGTT849780.8302528676786325No Hit
GCCTGTCTGAGCGTCGCT843020.8236482060185467No Hit
TGTAAACATCCTTGACTGGAAGCT826250.8072635645925651No Hit
ACTGGACTTGGAGTCAGAAGGC739020.7220380266326142No Hit
AGCAGCATTGTACAGGGCTATGA690280.6744180252550147No Hit
TGTAAACATCCCCGACTGGAAGCT662740.6475108681368552No Hit
CGCCTGTCTGAGCGTCGCT534680.5223935645583694No Hit
CAACGGAATCCCAAAAGCAGCTGT482860.4717643386374173No Hit
CATAAAGTAGAAAGCACTACT481200.4701424838510649No Hit
CACCCGGCTGTGTGCACATGTGC428060.4182235902686759No Hit
TCGTACGACTCTTAGCGG425500.4157224166222529No Hit
TTCAAGTAATTCAGGATAGGTT420720.4110522564543226No Hit
TGAGGTAGTAGTTTGTACAGTT394100.38504395861534635No Hit
ATCCCGGACGAGCCCCCA380530.3717857842473934No Hit
CCCATAAAGTAGAAAGCACTAC371750.3632075402569272No Hit
CATTGCACTTGTCTCGGTCTGA366840.3584103673647644No Hit
CTCCCACTGCTTCACTTGACTAG348440.34043318178109944No Hit
AACATTCAACGCTGTCGGTGAGTT331530.32391175742132905No Hit
TATTGCACTTGTCCCGGCCTGTAG306130.2990954251482263No Hit
CAACACCAGTCGATGGGCTGT299720.2928327208226126No Hit
CCCATAAAGTAGAAAGCACT281790.27531473508809556No Hit
CTTCTCACTACTGCACTTGACTAG263790.25772835788668413No Hit
AGCTCGGTCTGAGGCCCCTCAGT256990.2510846153883731No Hit
AACGGAATCCCAAAAGCAGCTG254770.24891562886686572No Hit
ACCCCCCACTGCTAAATTTGACTG245120.23948737664499795No Hit
CACTAGATTGTGAGCTCCTGGA243070.23748448368594832No Hit
ACTGGACTTGGAGTCAGAAGGCT232120.2267861042217564No Hit
TATTGCACTTGTCCCGGCCTGTT230600.22530103236919277No Hit
CCCCCCACTGCTAAATTTGACTGG225930.22073834450638216No Hit
CACTAGATTGTGAGCTCCTGGAA222840.21771934975347318No Hit
TTCTCACTACTGCACTTGACTAG220280.2152181761070502No Hit
TGTAAACATCCTACACTCTCAGCT213870.20895547178143653No Hit
ACTGGACTTGGAGTCAGAAGGCA211270.2064152172967882No Hit
TGTCAGTTTGTCAAATACCCCAA205380.2006605638681041No Hit
TGAGGTAGTAGTTTGTGCTGTT204190.1994979089308997No Hit
TCGTTTCCCGGGCGGCGCACCA196610.19209209008719424No Hit
CCCCCCACTGCTAAATTTGACTGGCT191460.1870604321656793No Hit
ACCGGGTGCTGTAGGCTTA183840.17961553248374848No Hit
TATTGCACTTGTCCCGGCCTGTAAT177010.17294247935676849No Hit
AACCGGGCGGAAACACCA169850.16594700931442927No Hit
TTCAAGTAATCCAGGATAGGCTT169570.16567344344685178No Hit
TACGCCTGTCTGAGCGTCGCT168650.16477458416766852No Hit
TATTGCACTTGTCCCGGCCTGTTT168140.1642763034802952No Hit
TGTAAACATCCCCGACTGGAAGC166140.16232226156902727No Hit
TAGCAGCACGTAAATATTGGC165960.16214639779701315No Hit
TAGCTTATCAGACTGATGTTGACA161860.1581406118789139No Hit
TGAGGTAGTAGGTTGTGTGGTT159360.15569805948982895No Hit
AGCAGCATTGTACAGGGCTATGT155620.15204400111575792No Hit
ATCCCACCGCTGCCACCA155370.15179974587684944No Hit
ACTGGACTTGGAGTCAGAAGG149920.14647498166864434No Hit
TATTGCACTTGTCCCGGCCTGTAAA143960.1406519367730659No Hit
GACCCGCGGGCGCTCTCCAGTCCT143890.14058354530617148No Hit
ACCACAGGGTAGAACCACGGAC143210.1399191710563404No Hit
AACATTCAACGCTGTCGGTGA141450.1381996141744246No Hit
CTACGCCTGTCTGAGCGTCGCT141360.13811168228841758No Hit
AACATTCAACGCTGTCGGTGAGA139820.13660707001674127No Hit
CAAAGAATTCTCCTTTTGGGCT136360.13322657751024772No Hit
CTCCCACTGCTTCACTTGACTAGCCT131420.12840009398941593No Hit
TTCAAGTAATCCAGGATAGGC124970.12209830882557685No Hit
CAACGGAATCCCAAAAGCAGCT120220.11745745928631551No Hit
TTCACAGTGGCTAAGTTCCG120080.11732067635252676No Hit
TGAGGGGCAGAGAGCGAGACTTT118010.11529824297436445No Hit
TGAGGTAGTAGGTTGTATAGT117840.11513214941190666No Hit
AGCAGCATTGTACAGGGCTATG117740.11503444731634327No Hit
TGTCAGTTTGTCAAATACCCCA117070.11437984327606851No Hit
CCCCCACTGCTAAATTTGACTGGCTT114410.11178096753408215No Hit
TGAGAACTGAATTCCATGGGTT113090.1104912998726453No Hit
TCCTGTCTGAGCGTCGCT112580.10999301918527199No Hit
ACCGGGTGCTGTAGGCTT111150.10859587921871541No Hit
CAAAGAATTCTCCTTTTGGGCTT110350.10781426245420826No Hit
CAAAGTGCTGTTCGTGCAGGT109620.10710103715659545No Hit
TTCACCACCTTCTCCACCCAGC109550.10703264568970108No Hit
TTCACAGTGGCTAAGTTCCGC109150.10664183730744749No Hit
TATTGCACTTGTCCCGGCCTGA107110.10464871455795419No Hit
CAAAGTGCTGTTCGTGCAGGTAG106990.10453147204327812No Hit
ACGCCTGTCTGAGCGTCGCT105800.10336881710607371No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)