FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947201

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947201
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9922508
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG118159111.908188937716151No Hit
CAACGGAATCCCAAAAGCAGCTG9762859.839095115871915No Hit
AACATTCAACGCTGTCGGTGAGT5533985.5771988291669805No Hit
AAGCTGCCAGTTGAAGAACTGT3896253.9266786179461888No Hit
AACATTCAACGCTGTCGGTGAG3143183.167727352802336No Hit
CCCCCACTGCTAAATTTGACTG2577682.5978109566653913No Hit
TAGCAGCACGTAAATATTGGCG2433872.4528778409652077No Hit
TTCAAGTAATCCAGGATAGGCT2419052.437942100928515No Hit
TATTGCACTTGTCCCGGCCTGTAA1584261.5966326255418488No Hit
TATTGCACTTGTCCCGGCCTGTAT1313871.3241309556011442No Hit
TAGCTTATCAGACTGATGTTGA1240081.249764676430596No Hit
TATTGCACTTGTCCCGGCCTGT1229011.2386082228404351No Hit
TAGCTTATCAGACTGATGTTGACT1121871.1306314895387335No Hit
TGAGGTAGTAGGTTGTATAGTT1110731.1194044892682375No Hit
TAGCTTATCAGACTGATGTTGAC1061511.0698000948953632No Hit
CGCCTGTCTGAGCGTCGCT1012531.020437574855067No Hit
GCCTGTCTGAGCGTCGCT987520.9952322537809998No Hit
CATAAAGTAGAAAGCACTACT749610.7554642435158531No Hit
TATTGCACTTGTCCCGGCCTGTA721680.7273161180620867No Hit
ATCCCGGACGAGCCCCCA661380.6665451920018608No Hit
TGAGGTAGTAGATTGTATAGTT659030.6641768391620344No Hit
ACTGGACTTGGAGTCAGAAGGC553310.5576311956614195No Hit
CAACGGAATCCCAAAAGCAGCTGT521660.5257340180526939No Hit
CCCATAAAGTAGAAAGCACT507900.5118665563182212No Hit
AGCAGCATTGTACAGGGCTATGA493160.4970114410590548No Hit
CCCATAAAGTAGAAAGCACTAC490580.49441129198384115No Hit
TGTAAACATCCTTGACTGGAAGCT488210.4920227829496333No Hit
TGTAAACATCCCCGACTGGAAGCT417970.4212342282818013No Hit
CAACACCAGTCGATGGGCTGT384830.38783541419165396No Hit
TCGTACGACTCTTAGCGG363050.36588531851019923No Hit
CACCCGGCTGTGTGCACATGTGC349550.352279887302686No Hit
CATTGCACTTGTCTCGGTCTGA344290.34697880818035115No Hit
AACATTCAACGCTGTCGGTGAGTT335860.33848297224854845No Hit
TGAGGTAGTAGTTTGTACAGTT332930.33553008977165855No Hit
TTCAAGTAATTCAGGATAGGTT321130.32363793508657285No Hit
CTCCCACTGCTTCACTTGACTAG306510.30890375699369554No Hit
GGAATACCGGGTGCTGTAGGCTTT294720.2970216804058006No Hit
AACGGAATCCCAAAAGCAGCTG270290.272400888968797No Hit
ACCGGGTGCTGTAGGCTT252450.254421563580498No Hit
TACGCCTGTCTGAGCGTCGCT218120.2198234559246513No Hit
GGAATACCGGGTGCTGTAGGCTT211850.21350448898605068No Hit
ACCGGGTGCTGTAGGCTTA211230.21287964696022418No Hit
CTTCTCACTACTGCACTTGACTAG209680.21131754189565782No Hit
TCCTGTCTGAGCGTCGCT203190.2047768568188607No Hit
CTACGCCTGTCTGAGCGTCGCT196560.1980950783813931No Hit
ATCCCACCGCTGCCACCA196550.1980850002842023No Hit
TTAATGCTAATCGTGATAGGGGTT195780.19730898680051456No Hit
ACCGGGTGCTGTAGGCTTT191070.19256220302367102No Hit
AACATTCAACGCTGTCGGTGA191040.1925319687320988No Hit
TGAGGTAGTAGTTTGTGCTGTT188870.1903450216417059No Hit
ACGCCTGTCTGAGCGTCGCT188830.1903047092529429No Hit
TTCTCACTACTGCACTTGACTAG186980.18844026127265406No Hit
TATTGCACTTGTCCCGGCCTGTT186750.1882084650372668No Hit
TGTAAACATCCTACACTCTCAGCT178240.17963200432793808No Hit
CCCCCCACTGCTAAATTTGACTGG178040.17943044238412303No Hit
TGTCAGTTTGTCAAATACCCCAA176650.1780295868746087No Hit
AGCTCGGTCTGAGGCCCCTCAGT169970.17129741795118733No Hit
TGAGGTAGTAGGTTGTGTGGTT169000.17031984252368454No Hit
TATTGCACTTGTCCCGGCCTGTAG166770.16807242685014717No Hit
CCCCCCACTGCTAAATTTGACTGGCT166550.16785070871195065No Hit
CACTAGATTGTGAGCTCCTGGA163550.16482727955472548No Hit
AACATTCAACGCTGTCGGTGAGA157400.15862924978241388No Hit
ACCCCCCACTGCTAAATTTGACTG155340.15655316176111927No Hit
TTCACAGTGGCTAAGTTCCG152360.1535498887982756No Hit
AACCGGGCGGAAACACCA152180.1533684830488421No Hit
ACTGGACTTGGAGTCAGAAGGCT150010.15118153595844921No Hit
ACCACAGGGTAGAACCACGGAC142410.14352218209347878No Hit
ACTGGACTTGGAGTCAGAAGGCA141150.1422523418474442No Hit
GCGTTCGCGCTTTCCCCTG133830.1348751747038148No Hit
TTCAAGTAATCCAGGATAGGCTT131230.13225486943421966No Hit
TTCAAGTAATCCAGGATAGGC128110.12911050311070546No Hit
TAGCTTATCAGACTGATGTTGACA124720.12569402816304104No Hit
ACTGGACTTGGAGTCAGAAGG120760.1217031016755038No Hit
CAACGGAATCCCAAAAGCAGCT119620.12055419859575826No Hit
CCCCCACTGCTAAATTTGACTGGCTT119490.1204231833322785No Hit
TTCACAGTGGCTAAGTTCTG118630.11955646697387394No Hit
TAGCAGCACGTAAATATTGGC113400.11428562214311139No Hit
CCCATAAAGTAGAAAGCACTA112430.1133080467156086No Hit
TTATAAAGCAATGAGACTGATT112090.11296539141112308No Hit
CAAAGAATTCTCCTTTTGGGCT110420.11128234918026773No Hit
TGAGGTAGTAGGTTGTATAGT109720.11057688237691518No Hit
CACTAGATTGTGAGCTCCTGGAA108900.10975047840727364No Hit
TTCACAGTGGCTAAGTTCCGC108160.10900469921515811No Hit
TGTAAACATCCCCGACTGGAAGC106790.10762399990002529No Hit
AGCAGCATTGTACAGGGCTATG101850.10264541988779449No Hit
CAAAGTGCTGTTCGTGCAGGT100210.1009926119485114No Hit
TATTGCACTTGTCCCGGCCTGA99250.10002511461819935No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)