FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947202

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947202
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9051420
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG8951389.889475905438042No Hit
CCCCCCACTGCTAAATTTGACTG8070088.915816523816153No Hit
AACATTCAACGCTGTCGGTGAGT3873554.279494267197854No Hit
AAGCTGCCAGTTGAAGAACTGT3140793.4699417328993682No Hit
TAGCAGCACGTAAATATTGGCG2943493.2519648850677574No Hit
TTCAAGTAATCCAGGATAGGCT2856253.1555822180387167No Hit
AACATTCAACGCTGTCGGTGAG2495202.7566945296981027No Hit
TATTGCACTTGTCCCGGCCTGTAA2264412.5017179624854444No Hit
TAGCTTATCAGACTGATGTTGACT2048052.2626836452180985No Hit
TATTGCACTTGTCCCGGCCTGTAT1887052.0848110020306208No Hit
CCCCCACTGCTAAATTTGACTG1668221.843047831169032No Hit
TAGCTTATCAGACTGATGTTGAC1640221.8121134584407752No Hit
TAGCTTATCAGACTGATGTTGA1249921.3809103985893925No Hit
TTAATGCTAATCGTGATAGGGGTT990831.0946680189406746No Hit
TGTAAACATCCTTGACTGGAAGCT964331.0653908447514313No Hit
TATTGCACTTGTCCCGGCCTGT820620.9066201767236522No Hit
TGAGGTAGTAGGTTGTATAGTT756520.835802559156464No Hit
TATTGCACTTGTCCCGGCCTGTA720970.7965269537818376No Hit
TGTAAACATCCCCGACTGGAAGCT665060.7347576402376644No Hit
CAACGGAATCCCAAAAGCAGCTGT609960.6738832139045586No Hit
CCCATAAAGTAGAAAGCACTAC578960.6396344440982741No Hit
AGCAGCATTGTACAGGGCTATGA563570.6226315870879928No Hit
CATAAAGTAGAAAGCACTACT515330.56933608207331No Hit
ACTGGACTTGGAGTCAGAAGGC479480.529729036990881No Hit
TGAGGTAGTAGATTGTATAGTT477180.5271879992310599No Hit
CAACGGAATCCCAAAAGCAGCT452550.4999767992204538No Hit
TTCAAGTAATTCAGGATAGGTT441210.48744837826550974No Hit
GCCTGTCTGAGCGTCGCT414770.45823749201782704No Hit
CACCCGGCTGTGTGCACATGTGC387930.42858468615974066No Hit
TCGTTTCCCGGCCAATGCACCA334340.3693785063559088No Hit
TGTAAACATCCTACACTCTCAGCT319940.35346940038137664No Hit
CAAAGAATTCTCCTTTTGGGCT319080.3525192732190087No Hit
TAGCTTATCAGACTGATGTTGACA286950.3170220805133338No Hit
TCGTACGACTCTTAGCGG284480.3142932269190912No Hit
AACATTCAACGCTGTCGGTGAGTT278560.30775281668511684No Hit
CCCATAAAGTAGAAAGCACT274160.3028917009706764No Hit
GGAATACCGGGTGCTGTAGGCTTT261400.2887944653987993No Hit
TATTGCACTTGTCCCGGCCTGTAG255640.2824308230089864No Hit
CATTGCACTTGTCTCGGTCTGA249200.27531591728148735No Hit
CCCCCACTGCTAAATTTGACTGGCTT232740.2571309253133763No Hit
CACTAGATTGTGAGCTCCTGGAA227920.251805794008012No Hit
CAAAGAATTCTCCTTTTGGGCTT214050.2364822315172647No Hit
TATTGCACTTGTCCCGGCCTGTAAT211090.23321202640027755No Hit
TGAGGTAGTAGTTTGTACAGTT208900.23079251653331745No Hit
CACTAGATTGTGAGCTCCTGGA208410.23025116501057294No Hit
TATTGCACTTGTCCCGGCCTGTAAA206300.22792003906569355No Hit
CTTCTCACTACTGCACTTGACTAG200910.2219651723155041No Hit
TCGTTTCCCGGGCGGCGCACCA197440.21813151969525224No Hit
AACGGAATCCCAAAAGCAGCTG192090.21222084490610313No Hit
CCCCCCACTGCTAAATTTGACTGGCT184050.20333826073698932No Hit
TAGCAGCACGTAAATATTGGC183500.20273062127268432No Hit
ACCCCCCACTGCTAAATTTGACTG181840.20089665488950906No Hit
TGTAAACATCCCCGACTGGAAGC174420.19269904611652094No Hit
TATTGCACTTGTCCCGGCCTGTT169920.18772745049947964No Hit
TTCAAGTAATCCAGGATAGGCTT168370.18601501200916543No Hit
CAACACCAGTCGATGGGCTGT168200.18582719617474386No Hit
ACTGGACTTGGAGTCAGAAGGCA167490.18504278886627734No Hit
GGAATACCGGGTGCTGTAGGCTT166760.18423628557729063No Hit
TGTCAGTTTGTCAAATACCCCAA162330.17934202589207No Hit
TTCTCACTACTGCACTTGACTAG159630.17635906852184519No Hit
ACTGGACTTGGAGTCAGAAGGCT156160.17252541590159334No Hit
TCCTGTCTGAGCGTCGCT154660.1708682173625796No Hit
TATTGCACTTGTCCCGGCCTGTTT153410.1694872185800681No Hit
TTAATGCTAATCGTGATAGGGGTTT152360.16832717960275848No Hit
ATCCCGGACGAGCCCCCA151180.16702351675206764No Hit
CCCCCCACTGCTAAATTTGACTGG148330.16387483952794146No Hit
AGCTCGGTCTGAGGCCCCTCAGT147690.16316776815129558No Hit
TGAGAACTGAATTCCATGGGTT145060.16026214671289146No Hit
CAACGGAATCCCAAAAGCAGCTGA143660.15871542807647862No Hit
CGCCTGTCTGAGCGTCGCT142260.15716870944006575No Hit
TGAGAACTGAATTCCATAGGCTGT137370.15176624220288087No Hit
ATGCACCTGGGCAAGGATTCTGA135500.14970026802424372No Hit
AGCAGCATTGTACAGGGCTATGT130240.14388902514743543No Hit
CTCCCACTGCTTCACTTGACTAG124950.13804463829984687No Hit
TTCAAGTAATCCAGGATAGGC121290.1340010738646533No Hit
CCCATAAAGTAGAAAGCACTA118860.131316412231451No Hit
TGAGGTAGTAGTTTGTGCTGTT118450.1308634446307872No Hit
AACATTCAACGCTGTCGGTGAGA116730.1289631903060514No Hit
TGATGTAGTAGGTTGTATAGTT113270.12514058567605965No Hit
TGTCAGTTTGTCAAATACCCCA110650.12224601222791563No Hit
AACATTCAACGCTGTCGGTGA108690.12008060613693763No Hit
TTCACAGTGGCTAAGTTCCG108540.11991488628303625No Hit
TTCACAGTGGCTAAGTTCTGC107130.11835711965636331No Hit
ATCACATTGCCAGGGATTTCCA105810.1168987849420312No Hit
ATCACATTGCCAGGGATTTCC104180.11509796252963624No Hit
CTACGCCTGTCTGAGCGTCGCT100300.11081134230872063No Hit
TTCACAGTGGCTAAGTTCCGC100070.1105572385327385No Hit
CTCCCACTGCTTCACTTGACTAGCCT99630.11007112696129447No Hit
CACCCGTAGAACCGACCTTGCG98310.10861279224696235No Hit
AACCGGGCGGAAACACCA97150.10733122537679171No Hit
ACTTGACTTGGAGTCAGAAGGC93020.10276840539937379No Hit
ACCACAGGGTAGAACCACGGAC92640.10234858176949031No Hit
TGAATACCGGGTGCTGTAGGCTTT91870.10149788651946325No Hit
TAGCTTATCAGACTGATGTTGACC91510.10110015887009995No Hit
TTAATGCTAATCGTGATAGGGGT91060.10060299930839581No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)