FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947204

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947204
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12276456
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG10944798.915268380386001No Hit
CCCCCCACTGCTAAATTTGACTG7847026.391926138944334No Hit
GGAATACCGGGTGCTGTAGGCTTT5429264.422497828363495No Hit
AACATTCAACGCTGTCGGTGAGT4573673.7255621654979256No Hit
TTCAAGTAATCCAGGATAGGCT4359373.551000386430742No Hit
AAGCTGCCAGTTGAAGAACTGT4203533.4240582135430615No Hit
GGAATACCGGGTGCTGTAGGCTT4051623.3003172902668325No Hit
AACATTCAACGCTGTCGGTGAG3348772.7277986415623534No Hit
TAGCAGCACGTAAATATTGGCG3276292.6687588014000134No Hit
CCCCCACTGCTAAATTTGACTG2507072.0421773189265697No Hit
TGAGGTAGTAGGTTGTATAGTT1835551.4951790647072738No Hit
TAGCTTATCAGACTGATGTTGA1630631.3282579272063535No Hit
TATTGCACTTGTCCCGGCCTGTAA1462161.1910277689261461No Hit
TAGCTTATCAGACTGATGTTGACT1443521.1758442338733588No Hit
TAGCTTATCAGACTGATGTTGAC1376061.1208935217134326No Hit
CATAAAGTAGAAAGCACTACT1220020.9937884353595207No Hit
TATTGCACTTGTCCCGGCCTGTAT1164020.9481726648146664No Hit
TGAATACCGGGTGCTGTAGGCTTT1129210.9198175760170525No Hit
TATTGCACTTGTCCCGGCCTGT1097550.8940283743125866No Hit
TGAGGTAGTAGATTGTATAGTT926270.7545092818318251No Hit
GGAATACCGGGTGCTGTAGGCTTA896020.7298686200642922No Hit
TGAATACCGGGTGCTGTAGGCTT846800.6897756160246898No Hit
GGAATACCGGGTGCTGTAGGCTTC746310.6079197449166112No Hit
TGTAAACATCCTTGACTGGAAGCT696030.5669632995059812No Hit
CGCCTGTCTGAGCGTCGCT689170.5613753676142366No Hit
CCCATAAAGTAGAAAGCACTAC681010.5547284981919863No Hit
CCCATAAAGTAGAAAGCACT672480.5477802388572077No Hit
CAACGGAATCCCAAAAGCAGCTGT670160.5458904426489208No Hit
TCGTACGACTCTTAGCGG641750.5227485847707188No Hit
TATTGCACTTGTCCCGGCCTGTA641210.5223087184118935No Hit
GGAATACCGGGTGCTGTAGGCT631850.5146843681922536No Hit
AGCAGCATTGTACAGGGCTATGA629180.5125094734180614No Hit
ACTGGACTTGGAGTCAGAAGGC615030.5009833456821741No Hit
GCCTGTCTGAGCGTCGCT605150.4929354204503319No Hit
CAACACCAGTCGATGGGCTGT559590.45582373284276834No Hit
TTCAAGTAATTCAGGATAGGTT509130.41472066531252993No Hit
TGTAAACATCCCCGACTGGAAGCT501580.40857068196228613No Hit
TGAGGTAGTAGTTTGTACAGTT431460.35145322070147933No Hit
CATTGCACTTGTCTCGGTCTGA406530.3311460571357076No Hit
ATCCCGGACGAGCCCCCA396180.32271528525822113No Hit
CACCCGGCTGTGTGCACATGTGC370430.3017401764808997No Hit
AACGGAATCCCAAAAGCAGCTG350460.2854732668776722No Hit
TTCACAGTGGCTAAGTTCCG297410.24226046995973433No Hit
TGTAAACATCCTACACTCTCAGCT295180.24044398481125173No Hit
AACATTCAACGCTGTCGGTGAGTT294430.23983305931288312No Hit
ACCGGGTGCTGTAGGCTT290650.23675399480110548No Hit
CTCCCACTGCTTCACTTGACTAG282140.22982202681294991No Hit
TGAGGTAGTAGTTTGTGCTGTT243780.19857522398972474No Hit
TTCAAGTAATCCAGGATAGGC238620.19437205656094886No Hit
GTCAGGATGGCCGAGCGGTCTAAGG236360.19253113439253153No Hit
CTACGCCTGTCTGAGCGTCGCT228880.18643817075546884No Hit
ACCGGGTGCTGTAGGCTTA219990.17919666718147323No Hit
TGAGGTAGTAGGTTGTGTGGTT215320.17539263774496483No Hit
CTTCTCACTACTGCACTTGACTAG214180.17446403098744456No Hit
CCCCCACTGCTAAATTTGACTGGCTT211690.1724357583328609No Hit
CAACGGAATCCCAAAAGCAGCT211090.171947017934166No Hit
TTCTCACTACTGCACTTGACTAG210530.17149086022871748No Hit
ACCGGGTGCTGTAGGCTTT205710.16756464569253537No Hit
TTCACAGTGGCTAAGTTCCGC203190.16551193601801695No Hit
AGCTCGGTCTGAGGCCCCTCAGT201340.16400498645537442No Hit
TTCACAGTGGCTAAGTTCTG194810.15868586178291194No Hit
ACTGGACTTGGAGTCAGAAGGCT191400.15590818718366278No Hit
TAGCAGCACGTAAATATTGGC188840.153822894815898No Hit
TGAATACCGGGTGCTGTAGGCTTA188140.15325269768408734No Hit
TCCACCACGTTCCCGTGG187900.1530572015246094No Hit
TGAGGTAGTAGGTTGTATAGT185600.1511836966629457No Hit
CAAAGAATTCTCCTTTTGGGCT185500.1511022399298299No Hit
CCCCCCACTGCTAAATTTGACTGGCT184490.15027952692536023No Hit
TACGCCTGTCTGAGCGTCGCT179610.14630443834930862No Hit
AACCGGGCGGAAACACCA176710.14394219308895012No Hit
CACTAGATTGTGAGCTCCTGGA176100.14344530701694364No Hit
TATTGCACTTGTCCCGGCCTGTT174580.14220716467358333No Hit
AACATTCAACGCTGTCGGTGA173750.1415310737887221No Hit
TTCAAGTAATCCAGGATAGGCTT170890.1392014112216099No Hit
CCCATAAAGTAGAAAGCACTA169700.13823207609753174No Hit
GGAATACCGGGTGCTGTAGGCTA166730.13581281112399213No Hit
ACGCCTGTCTGAGCGTCGCT163510.133189904317663No Hit
ACTGGACTTGGAGTCAGAAGG162580.13243235669968598No Hit
TGAATACCGGGTGCTGTAGGCTTC162470.13234275429325856No Hit
ACCCCCCACTGCTAAATTTGACTG159690.130078257112639No Hit
CCCCCCACTGCTAAATTTGACTGG159010.12952435132745152No Hit
TTCACAGTGGCTAAGTTCTGC154780.1260787315166527No Hit
ACCACAGGGTAGAACCACGGAC154330.12571217621763156No Hit
ACTGGACTTGGAGTCAGAAGGCA153420.12497091994627765No Hit
TAGCTTATCAGACTGATGTTGACA153200.12479171513342287No Hit
ATCCCACCGCTGCCACCA152130.12392012808908369No Hit
TGTAAACATCCTACACTCAGCT151340.12327661989746878No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC146880.11964364960050361No Hit
TATTGCACTTGTCCCGGCCTGTAG143310.11673564422826914No Hit
TGTAAACATCCCCGACTGGAAGC141810.11551379323153196No Hit
TCGTTTCCCGGCCAATGCACCA140110.11412902876856316No Hit
TTATAAAGCAATGAGACTGATT139430.11357512298337566No Hit
AGCAGCATTGTACAGGGCTATG139130.11333075278402822No Hit
CACTAGATTGTGAGCTCCTGGAA137200.11175863783489307No Hit
AGCAGCATTGTACAGGGCTATGT136270.11100109021691601No Hit
TGAATACCGGGTGCTGTAGGCT134960.1099340070130989No Hit
CAAAGTGCTGTTCGTGCAGGT133690.10889950650252808No Hit
AGAGGTAGTAGGTTGCATAGTT127900.1041831616551226No Hit
AATGACACGATCACTCCCGTTG126370.10293687363845071No Hit
AACATTCAACGCTGTCGGTGAGA122930.1001347620192668No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)