FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947205

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947205
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7068608
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG5735468.113987930862766No Hit
CAACGGAATCCCAAAAGCAGCTG5683748.040819352268509No Hit
GGAATACCGGGTGCTGTAGGCTTT4970497.0317805146359795No Hit
GGAATACCGGGTGCTGTAGGCTT3556455.03133007234239No Hit
GCCTGTCTGAGCGTCGCT2412523.413005785580414No Hit
AACATTCAACGCTGTCGGTGAGT2319393.281254244117088No Hit
CCCCCACTGCTAAATTTGACTG2302263.257020335545556No Hit
AACATTCAACGCTGTCGGTGAG1645812.328336781442683No Hit
TTCAAGTAATCCAGGATAGGCT1526892.1600999800809437No Hit
AAGCTGCCAGTTGAAGAACTGT1177811.6662545157405815No Hit
TAGCAGCACGTAAATATTGGCG1115021.577425145092216No Hit
TATTGCACTTGTCCCGGCCTGTAA1079051.5265381812090866No Hit
TATTGCACTTGTCCCGGCCTGTAT928831.3140210915642796No Hit
GGAATACCGGGTGCTGTAGGCTTA749851.060817066104104No Hit
TATTGCACTTGTCCCGGCCTGT742621.0505887439224244No Hit
TGAGGTAGTAGGTTGTATAGTT731011.0341640107925067No Hit
TAGCTTATCAGACTGATGTTGAC668390.9455751401124521No Hit
TAGCTTATCAGACTGATGTTGACT644670.9120183210046447No Hit
TAGCTTATCAGACTGATGTTGA613250.8675682680380634No Hit
GGAATACCGGGTGCTGTAGGCT555330.7856285141289489No Hit
TATTGCACTTGTCCCGGCCTGTA522310.738914932048856No Hit
TGAGGTAGTAGATTGTATAGTT438500.6203484476717339No Hit
ACTGGACTTGGAGTCAGAAGGC338050.47824126051409277No Hit
AGCAGCATTGTACAGGGCTATGA329000.46543817396579357No Hit
CACCCGGCTGTGTGCACATGTGC324510.45908614539100207No Hit
CATTGCACTTGTCTCGGTCTGA281330.3979991534401116No Hit
TGAATACCGGGTGCTGTAGGCTTT275160.389270419296133No Hit
CAACGGAATCCCAAAAGCAGCTGT270410.3825505672403958No Hit
TTCAAGTAATTCAGGATAGGTT263760.37314277436236387No Hit
TGTAAACATCCCCGACTGGAAGCT259940.36773859860385527No Hit
TGTAAACATCCTTGACTGGAAGCT259670.36735662806595015No Hit
CAACGGAATCCCAAAAGCAGCT258450.3656306871168977No Hit
GTAATACCGGGTGCTGTAGGCTTT255950.362093922877036No Hit
CATAAAGTAGAAAGCACTACT255380.36128754063034757No Hit
TCGTACGACTCTTAGCGG247580.3502528362019792No Hit
ACCGGGTGCTGTAGGCTT215410.30474175396343944No Hit
TGAATACCGGGTGCTGTAGGCTT198570.2809181100437314No Hit
ACCGGGTGCTGTAGGCTTT189110.26753499416009485No Hit
TGATGTAGTAGGTTGTATAGTT186590.26396993580631434No Hit
ACCGGGTGCTGTAGGCTTA184820.26146590672449227No Hit
GTAATACCGGGTGCTGTAGGCTT182510.25819793656686013No Hit
CGCGACCTCAGATCAGACGTGG177180.2506575552074751No Hit
CCCATAAAGTAGAAAGCACT171520.24265032096842828No Hit
CTCCCACTGCTTCACTTGACTAG156430.22130241201662335No Hit
TGAGGTAGTAGTTTGTACAGTT153700.2174402654666944No Hit
CACTAGATTGTGAGCTCCTGGA153480.2171290302135866No Hit
CCCCCACTGCTAAATTTGACTGGCTT153410.21703000081487048No Hit
CGCCTGTCTGAGCGTCGCT151800.21475232464439956No Hit
AACATTCAACGCTGTCGGTGAGTT145370.20565576701947538No Hit
GGAATACCGGGTGCTGTAGGCTA141500.20018085597616955No Hit
CAACACCAGTCGATGGGCTGT138550.19600747417313283No Hit
AACGGAATCCCAAAAGCAGCTG127390.18021935860639038No Hit
CCCATAAAGTAGAAAGCACTAC127300.18009203509375538No Hit
TCCTGTCTGAGCGTCGCT127080.17978079984064754No Hit
TATTGCACTTGTCCCGGCCTGTT117770.16660988981140276No Hit
CCCCCCACTGCTAAATTTGACTGG117110.1656761840520793No Hit
TGATGTAGTAGATTGTATAGTT115090.16281847854627107No Hit
TGAGGTAGTAGTTTGTGCTGTT108650.15370777386438744No Hit
CACTAGATTGTGAGCTCCTGGAA107790.15249112696587502No Hit
ACTGGACTTGGAGTCAGAAGGCT99860.14127251079703387No Hit
CGCGACCTCAGATCAGACGTGGCG99640.14096127554392604No Hit
CCCCCCACTGCTAAATTTGACTGGCT98770.1397304815884542No Hit
CCCCCCACTGCTAAATTTGACTGTA98650.13956071690494082No Hit
TTATAAAGCAATGAGACTGATT98640.1395465698479814No Hit
ACCCCCCACTGCTAAATTTGACTG98260.1390089816835224No Hit
TATTGCACTTGTCCCGGCCTGTAG97610.13808942298115837No Hit
TTCTCACTACTGCACTTGACTAG96260.13617957029163308No Hit
CTCCCACTGCTTCACTTGACTAGCCT95630.13528830570318795No Hit
TACCACCCTGAACGCGCCCGA95490.13509024690575572No Hit
ACTTGACTTGGAGTCAGAAGGC95130.13458095285521562No Hit
ATCCCGGACGAGCCCCCA94650.13390189412116218No Hit
CCCCCCACTGCTAAATTTGACTGT92550.1309310121596784No Hit
TAGCAGCACGTAAATATTGGC85750.1213110134272547No Hit
TTCAAGTAATCCAGGATAGGCTT83570.11822695501009534No Hit
GAATACCGGGTGCTGTAGGCTTT82970.11737813159252854No Hit
TATTGCACTTGTCCCGGCCTGTAAT81550.11536924950428713No Hit
CAACGGAATCCCAAAAGCAGCTGA79180.11201639700489828No Hit
ACCACAGGGTAGAACCACGGAC79060.11184663232138492No Hit
CTTCTCACTACTGCACTTGACTAG79010.11177589703658768No Hit
ACTGGACTTGGAGTCAGAAGGCA77360.109441632638279No Hit
CGCGACCTCAGATCAGACGTGGC77260.10930016206868454No Hit
TAGCTTATCAGACTGATGTTGACA77100.10907380915733338No Hit
AGCAGCATTGTACAGGGCTATGT76400.10808351517017212No Hit
TGTAAACATCCTACACTCTCAGCT76160.1077439858031454No Hit
AATGCACCCGGGCAAGGATTCT73390.10382525102537868No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)