FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947206

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947206
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10313033
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG6655706.453678563813381No Hit
CAACGGAATCCCAAAAGCAGCTG5989105.807311971172787No Hit
AACATTCAACGCTGTCGGTGAGT4438364.3036418093493936No Hit
TTCAAGTAATCCAGGATAGGCT3089892.9961021166130273No Hit
AAGCTGCCAGTTGAAGAACTGT2922252.8335505180677694No Hit
TAGCAGCACGTAAATATTGGCG2632972.5530510762449805No Hit
AACATTCAACGCTGTCGGTGAG2144442.0793494988331758No Hit
CCCCCACTGCTAAATTTGACTG2013071.951966991669667No Hit
TAGCTTATCAGACTGATGTTGAC1796091.7415730173655026No Hit
TAGCTTATCAGACTGATGTTGACT1734551.6819009499921118No Hit
TATTGCACTTGTCCCGGCCTGTAA1414431.3714975992028728No Hit
TATTGCACTTGTCCCGGCCTGTAT1191271.1551112073431744No Hit
GGAATACCGGGTGCTGTAGGCTTT1185781.1497878461166564No Hit
TAGCTTATCAGACTGATGTTGA961620.932431807403312No Hit
TGTAAACATCCTTGACTGGAAGCT926120.8980093440988699No Hit
TATTGCACTTGTCCCGGCCTGT802590.778228868267948No Hit
GGAATACCGGGTGCTGTAGGCTT786360.7624914998332692No Hit
TGAATACCGGGTGCTGTAGGCTTT691610.6706174604502866No Hit
TGAGGTAGTAGGTTGTATAGTT683900.6631414832086739No Hit
TGTAAACATCCCCGACTGGAAGCT586790.5689790772510861No Hit
TATTGCACTTGTCCCGGCCTGTA584100.5663707272147777No Hit
AGCAGCATTGTACAGGGCTATGA577160.5596413780504726No Hit
ACTGGACTTGGAGTCAGAAGGC575950.5584681053575606No Hit
CATAAAGTAGAAAGCACTACT526750.5107614801581649No Hit
TGAATACCGGGTGCTGTAGGCTT459700.4457466586211835No Hit
TGAGGTAGTAGATTGTATAGTT449660.4360114042105751No Hit
TTAATGCTAATCGTGATAGGGGTT433640.42047766161516215No Hit
TTCAAGTAATTCAGGATAGGTT420510.4077461984267868No Hit
CCCATAAAGTAGAAAGCACTAC419610.40687351625850515No Hit
CAACGGAATCCCAAAAGCAGCTGT393900.3819438956512599No Hit
CGCCTGTCTGAGCGTCGCT391060.37919009858690456No Hit
AACATTCAACGCTGTCGGTGAGTT378510.36702103057364405No Hit
CCCCCACTGCTAAATTTGACTGGCTT358000.34713357360535935No Hit
TTTAAACATCCTTGACTGGAAGCT277960.2695230394395131No Hit
CTCCCACTGCTTCACTTGACTAG267350.2592350863223263No Hit
ACTTGACTTGGAGTCAGAAGGC260080.252185753696318No Hit
CCCCCCACTGCTAAATTTGACTGGCT259830.25194334198290647No Hit
TTAATACCGGGTGCTGTAGGCTTT253770.24606728204981018No Hit
TTCTCACTACTGCACTTGACTAG246320.2388434129901456No Hit
CTACGCCTGTCTGAGCGTCGCT238210.23097957700707444No Hit
TGTAAACATCCTACACTCTCAGCT229980.22299938340156578No Hit
TAGCTTATCAGACTGATGTTGACA220830.21412711469070253No Hit
TTCAAGTAATCCAGGATAGGCTT218560.21192601633292554No Hit
CATTGCACTTGTCTCGGTCTGA218280.21165451521390458No Hit
TGATGTAGTAGGTTGTATAGTT217650.21104363769610746No Hit
GTAATACCGGGTGCTGTAGGCTTT217230.210636386017576No Hit
TGTCAGTTTGTCAAATACCCCAA215940.20938554157637232No Hit
CTTCTCACTACTGCACTTGACTAG211800.20537120360227687No Hit
CTCCCACTGCTTCACTTGACTAGCCT209800.20343190989498433No Hit
CCCCCCACTGCTAAATTTGACTGG208650.20231681601329113No Hit
CCCATAAAGTAGAAAGCACT207130.20084295279574885No Hit
CAAAGAATTCTCCTTTTGGGCT206660.20038721877453508No Hit
TGAGGTAGTAGTTTGTACAGTT197100.1911173948536769No Hit
CAAAGAATTCTCCTTTTGGGCTT197000.19102043016831227No Hit
GCCTGTCTGAGCGTCGCT194500.18859631303419663No Hit
GGAATACCGGGTGCTGTAGGCTTA192620.18677337694934168No Hit
TCGTACGACTCTTAGCGG192480.1866376263898312No Hit
CACCCGGCTGTGTGCACATGTGC187590.181896053275501No Hit
TACGCCTGTCTGAGCGTCGCT182040.1765145132377643No Hit
TTTAAACATCCCCGACTGGAAGCT173840.168563409037865No Hit
TTAATACCGGGTGCTGTAGGCTT169040.16390910414036297No Hit
TATTGCACTTGTCCCGGCCTGTAG168820.1636957818325608No Hit
TGTAAACATCCCCGACTGGAAGC167300.1622219186150185No Hit
CGCGACCTCAGATCAGACGTGGCG166330.1612813611669816No Hit
CGCCCCACTGCTAAATTTGACTG161290.1563943410246045No Hit
TAGCAGCACGTAAATATTGGC158330.1535241863378116No Hit
CCCCCCACTGCTAAATTTGACTGTA153220.14856929091567922No Hit
ATCAGCATTGTACAGGGCTATGA151520.1469208912644806No Hit
ACTGGACTTGGAGTCAGAAGGCA150830.14625183493546468No Hit
GTAATACCGGGTGCTGTAGGCTT145650.14122906423357706No Hit
TATTGCACTTGTCCCGGCCTGTT145490.14107392073699365No Hit
TGATGTAGTAGATTGTATAGTT143670.13930916346335748No Hit
TGCAAGTAATCCAGGATAGGCT143210.1388631259106802No Hit
TTATAAAGCAATGAGACTGATT142920.1385819283231228No Hit
ACTGGACTTGGAGTCAGAAGGCT140450.13618690059461652No Hit
CAACGGAATCCCAAAAGCAGCT135630.1315132027600416No Hit
ACCCCCCACTGCTAAATTTGACTG133370.12932180087080106No Hit
ACGCCTGTCTGAGCGTCGCT133150.1291084785629989No Hit
TGTCAGTTTGTCAAATACCCCA130750.12678132611424786No Hit
AACATTCAACGCTGTCGGTGAGA128390.1244929595396427No Hit
TCGTTTCCCGGCCAATGCACCA127380.12351361621745999No Hit
ACCACAGGGTAGAACCACGGAC125380.12157432251016748No Hit
CAACACCAGTCGATGGGCTGT122210.11850054198410884No Hit
AACATTCAACGCTGTCGGTGA122170.11846175610996298No Hit
TTAGGTAGTAGGTTGTATAGTT121820.11812237971118679No Hit
CACTAGATTGTGAGCTCCTGGA120540.1168812317385196No Hit
GGAATACCGGGTGCTGTAGGCT120030.11638671184316No Hit
TGAATACCGGGTGCTGTAGGCTTA119810.11617338953535783No Hit
TTATGTAGTAGGTTGTATAGTT119460.11583401313658163No Hit
CCAAGTCCTTCTGATCGAGGCCC119420.11579522726243578No Hit
AGCTCGGTCTGAGGCCCCTCAGT118340.11474800866049784No Hit
TTCAAGTAATCCAGGATAGGC117930.11435045345050288No Hit
TATTGCACTTGTCCCGGCCTGTAAT117000.11344868187661186No Hit
AACCGGGCGGAAACACCA115600.1120911762815071No Hit
TGAGGTAGTAGTTTGTGCTGTT114990.11149969170078289No Hit
AGCAGCATTGTACAGGGCTATGT114720.1112378870502984No Hit
CACTAGATTGTGAGCTCCTGGAA113520.11007431082592288No Hit
TCCTGTCTGAGCGTCGCT109800.10646722453035883No Hit
TAGCAGCACGTAAATATTGGCGT107290.10403341092770672No Hit
CCCGCCACTGCTAAATTTGACTG107190.10393644624234208No Hit
CGCGACCTCAGATCAGACGTGG107050.10380069568283162No Hit
CCCCCCACTGCTAAATTTGACTGT104360.1011923456465232No Hit
CAAAGTGCTGTTCGTGCAGGTAG103800.10064934340848128No Hit
CGACGGAATCCCAAAAGCAGCTG103580.10043602110067912No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)