FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947208

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947208
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10649859
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG7100626.667337098078012No Hit
CAACGGAATCCCAAAAGCAGCTG6517416.11971482439345No Hit
AAGCTGCCAGTTGAAGAACTGT3838523.6042918502489094No Hit
TAGCAGCACGTAAATATTGGCG3729803.502205991647401No Hit
AACATTCAACGCTGTCGGTGAGT3509293.295151607171513No Hit
TTCAAGTAATCCAGGATAGGCT3025782.841145596387708No Hit
CCCCCACTGCTAAATTTGACTG2312522.171409029922368No Hit
GGAATACCGGGTGCTGTAGGCTTT2166762.034543368132855No Hit
AACATTCAACGCTGTCGGTGAG1749821.6430452271715523No Hit
TAGCTTATCAGACTGATGTTGAC1713951.609364030077769No Hit
GGAATACCGGGTGCTGTAGGCTT1502361.4106853433458604No Hit
TAGCTTATCAGACTGATGTTGACT1373331.2895288097241475No Hit
TATTGCACTTGTCCCGGCCTGTAA1243261.1673957373520156No Hit
TAGCTTATCAGACTGATGTTGA1141151.0715165336930752No Hit
TGAATACCGGGTGCTGTAGGCTTT1129901.0609530135563296No Hit
GCCTGTCTGAGCGTCGCT1078761.012933598463604No Hit
TATTGCACTTGTCCCGGCCTGTAT1037810.9744823851658505No Hit
TGTAAACATCCTTGACTGGAAGCT1021860.9595056610608648No Hit
TGAGGTAGTAGGTTGTATAGTT956230.8978804320320108No Hit
TTAATGCTAATCGTGATAGGGGTT952060.8939648872346573No Hit
TATTGCACTTGTCCCGGCCTGT881700.8278982848505319No Hit
TGAATACCGGGTGCTGTAGGCTT778540.7310331526454952No Hit
AGCAGCATTGTACAGGGCTATGA682950.6412760957680285No Hit
TATTGCACTTGTCCCGGCCTGTA642520.6033131518454846No Hit
TGTAAACATCCCCGACTGGAAGCT594960.5586552836051633No Hit
TGAGGTAGTAGATTGTATAGTT590950.5548899755386433No Hit
CATAAAGTAGAAAGCACTACT578720.5434062554255413No Hit
ACTGGACTTGGAGTCAGAAGGC549990.5164293724452126No Hit
TTCAAGTAATTCAGGATAGGTT535700.5030113544226267No Hit
TCCTGTCTGAGCGTCGCT515120.48368715491914027No Hit
CAACGGAATCCCAAAAGCAGCTGT454520.4267849931158713No Hit
CGCCTGTCTGAGCGTCGCT443600.4165313362364704No Hit
CCCATAAAGTAGAAAGCACTAC407720.3828407493470101No Hit
TCGTACGACTCTTAGCGG390800.36695321506134493No Hit
CCCCCACTGCTAAATTTGACTGGCTT352160.3306710445650032No Hit
TGTAAACATCCTACACTCTCAGCT335470.3149994755799114No Hit
AACATTCAACGCTGTCGGTGAGTT335060.31461449395715No Hit
GGAATACCGGGTGCTGTAGGCTTA325710.3058350349990549No Hit
TTCTCACTACTGCACTTGACTAG295630.27759052960231684No Hit
CATTGCACTTGTCTCGGTCTGA286260.26879229105286745No Hit
CAACGGAATCCCAAAAGCAGCT277330.26040720351321084No Hit
TGAGGTAGTAGTTTGTACAGTT267410.2510925262015206No Hit
CAAAGAATTCTCCTTTTGGGCT258510.2427356080488953No Hit
CTCCCACTGCTTCACTTGACTAG246450.23141151446230418No Hit
CCCCCCACTGCTAAATTTGACTGGCT244530.2296086736922996No Hit
CCCCCCACTGCTAAATTTGACTGG230070.21603102914320274No Hit
CCCATAAAGTAGAAAGCACT229430.2154300822198679No Hit
CTTCTCACTACTGCACTTGACTAG224240.21055677826344932No Hit
GGAATACCGGGTGCTGTAGGCT222110.2085567517842255No Hit
CTACGCCTGTCTGAGCGTCGCT217710.20442524168629836No Hit
CAAAGAATTCTCCTTTTGGGCTT217120.20387124374134907No Hit
AACCGGGCGGAAACACCA214030.200969796877123No Hit
TGATGTAGTAGGTTGTATAGTT211360.19846272143133536No Hit
TAGCTTATCAGACTGATGTTGACA208200.19549554599736954No Hit
TACGCCTGTCTGAGCGTCGCT202900.19051895428850277No Hit
AGCTCGGTCTGAGGCCCCTCAGT199650.187467270693443No Hit
TTCAAGTAATCCAGGATAGGCTT195800.18385219935775676No Hit
CACCCGGCTGTGTGCACATGTGC192320.1805845504621235No Hit
TGTAAACATCCCCGACTGGAAGC187540.1760962281284663No Hit
TAGCAGCACGTAAATATTGGC183370.17218068333111264No Hit
ACCGGGTGCTGTAGGCTT177710.16686605897787005No Hit
TGAGGTAGTAGTTTGTGCTGTT176900.16610548552802437No Hit
TGAATACCGGGTGCTGTAGGCTTA175530.16481908352026067No Hit
CGCGACCTCAGATCAGACGTGG174530.16388010395254998No Hit
CGCGACCTCAGATCAGACGTGGCG169620.1592697142750904No Hit
TTCACAGTGGCTAAGTTCCG164520.1544809184797658No Hit
TATTGCACTTGTCCCGGCCTGTT159540.14980480023256645No Hit
TCGTTTCCCGGCCAATGCACCA152390.1430910963234349No Hit
ACTGGACTTGGAGTCAGAAGGCA148990.1398985657932185No Hit
ACCGGGTGCTGTAGGCTTT148290.13924128009582099No Hit
TGTCAGTTTGTCAAATACCCCA147410.13841497807623557No Hit
CTCCCACTGCTTCACTTGACTAGCCT146850.13788914951831757No Hit
TTATAAAGCAATGAGACTGATT144990.13614264752237565No Hit
TATTGCACTTGTCCCGGCCTGTAG142890.13417079043018318No Hit
CACTAGATTGTGAGCTCCTGGA140320.13175761294116664No Hit
ACGCCTGTCTGAGCGTCGCT138340.12989843339709942No Hit
AACGGAATCCCAAAAGCAGCTG136690.12834911711037678No Hit
ACTTGACTTGGAGTCAGAAGGC134730.12650871715766376No Hit
ACCGGGTGCTGTAGGCTTA133350.12521292535422301No Hit
TGATGTAGTAGATTGTATAGTT133100.12497818046229532No Hit
AGCAGCATTGTACAGGGCTATGT131810.12376689681994851No Hit
TAGCAGCACGTAAATATTGGCGT130850.12286547643494622No Hit
ACCCCCCACTGCTAAATTTGACTG129430.12153212544879703No Hit
CAACACCAGTCGATGGGCTGT129030.12115653362171275No Hit
CACTAGATTGTGAGCTCCTGGAA128410.1205743662897321No Hit
TTCAAGTAATCCAGGATAGGC125030.1174006153508699No Hit
CCCCCCACTGCTAAATTTGACTGTA124980.11735366637248437No Hit
TGTAAACATCCTACACTCAGCT121480.11406723788549689No Hit
ACTGGACTTGGAGTCAGAAGGCT117260.11010474410975771No Hit
TGTCAGTTTGTCAAATACCCCAA116260.10916576454204699No Hit
ACTGGACTTGGAGTCAGAAGG116020.10894040944579642No Hit
ATCACATTGCCAGGGATTTCCA115860.1087901727149627No Hit
TGAATACCGGGTGCTGTAGGCT115030.10801081967376282No Hit
ACCACAGGGTAGAACCACGGAC113430.1065084523654257No Hit
GCGTTCGCGCTTTCCCCTG113030.10613286053834141No Hit
CAAAGTGCTGTTCGTGCAGGT109080.1024238912458841No Hit
AACATTCAACGCTGTCGGTGAGA108340.10172904636577818No Hit
TTCACAGTGGCTAAGTTCTGC107100.1005647117018169No Hit
TTAATGCTAATCGTGATAGGGGTTT106610.10010461171363866No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)