FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947209

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947209
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6388640
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG3077014.816377194520274No Hit
CCCCCCACTGCTAAATTTGACTG2753644.310213128302737No Hit
AACATTCAACGCTGTCGGTGAGT2610564.086253099251171No Hit
TTCAAGTAATCCAGGATAGGCT2243183.5112011320093166No Hit
AAGCTGCCAGTTGAAGAACTGT2219833.474651882090711No Hit
TAGCAGCACGTAAATATTGGCG2159463.3801560269478323No Hit
AACATTCAACGCTGTCGGTGAG1170781.8325966089809413No Hit
CCCCCACTGCTAAATTTGACTG1161601.8182273535525557No Hit
TAGCTTATCAGACTGATGTTGAC933131.4606082045630995No Hit
CGCCTGTCTGAGCGTCGCT897811.4053225725663052No Hit
TAGCTTATCAGACTGATGTTGA847171.3260568759548197No Hit
TATTGCACTTGTCCCGGCCTGT755001.181785168674397No Hit
TGAGGTAGTAGGTTGTATAGTT731431.1448915575145884No Hit
TAGCTTATCAGACTGATGTTGACT727181.1382391244458916No Hit
CATAAAGTAGAAAGCACTACT649021.015896967116632No Hit
TATTGCACTTGTCCCGGCCTGTAA546840.8559568233614666No Hit
TATTGCACTTGTCCCGGCCTGTAT493000.7716822359688448No Hit
TGTAAACATCCTTGACTGGAAGCT487730.7634332189636605No Hit
TCGTACGACTCTTAGCGG483010.7560451050614841No Hit
TGAGGTAGTAGATTGTATAGTT402920.6306819604798518No Hit
TATTGCACTTGTCCCGGCCTGTA388730.6084706604222495No Hit
AGCAGCATTGTACAGGGCTATGA387850.6070932154574369No Hit
ACTGGACTTGGAGTCAGAAGGC376040.5886072779183049No Hit
TTCAAGTAATTCAGGATAGGTT355590.5565973352701045No Hit
ACCGGGTGCTGTAGGCTT308960.4836084049187307No Hit
CCCATAAAGTAGAAAGCACTAC285930.44756004407823885No Hit
CATTGCACTTGTCTCGGTCTGA274300.4293558566455458No Hit
TGTAAACATCCCCGACTGGAAGCT261240.4089133211450324No Hit
CCCATAAAGTAGAAAGCACT246840.3863733126299181No Hit
TACGCCTGTCTGAGCGTCGCT227670.35636692629417216No Hit
CTACGCCTGTCTGAGCGTCGCT222440.34818052042375214No Hit
AACATTCAACGCTGTCGGTGAGTT220450.3450656164692329No Hit
GCCTGTCTGAGCGTCGCT216100.33825665556362544No Hit
ACGCCTGTCTGAGCGTCGCT210830.33000763855844123No Hit
CAACGGAATCCCAAAAGCAGCTGT184810.2892790953943249No Hit
TGAGGTAGTAGTTTGTACAGTT182630.2858667885496757No Hit
ACCGGGTGCTGTAGGCTTT181220.2836597460492374No Hit
CTCCCACTGCTTCACTTGACTAG168740.264125072002805No Hit
GCGTTCGCGCTTTCCCCTG166120.26002404267574947No Hit
CAACACCAGTCGATGGGCTGT164160.2569560970723033No Hit
TTCTCACTACTGCACTTGACTAG157800.24700092664479453No Hit
TGTAAACATCCTACACTCTCAGCT155080.24274336948082847No Hit
ACCGGGTGCTGTAGGCTTA152430.2385953818026998No Hit
TGATGTAGTAGGTTGTATAGTT152420.23857972901900876No Hit
TTCACAGTGGCTAAGTTCCG140010.21915462445841366No Hit
ATCCCGGACGAGCCCCCA138130.21621190112449598No Hit
TGTCAGTTTGTCAAATACCCCAA137520.21525708131934185No Hit
TTATAAAGCAATGAGACTGATT134670.2107960379673921No Hit
TTCAAGTAATCCAGGATAGGCTT133600.20912119011244962No Hit
TGAGGTAGTAGTTTGTGCTGTT132290.20707067544892183No Hit
CACCCGGCTGTGTGCACATGTGC124750.19526847654586893No Hit
TTCAAGTAATCCAGGATAGGC115220.18035137368829673No Hit
CTTCTCACTACTGCACTTGACTAG114470.17917741491146785No Hit
TATTGCACTTGTCCCGGCCTGTT108320.1695509529414711No Hit
TAGTGGGTTATCAGAACT102430.1603314633474417No Hit
TCCTGTCTGAGCGTCGCT102110.15983057426932806No Hit
CCCCCACTGCTAAATTTGACTGGCTT100020.15655914247789826No Hit
ACCACAGGGTAGAACCACGGAC99670.15601129504871145No Hit
TAGCAGCACGTAAATATTGGC98070.1535068496581432No Hit
ACTGGACTTGGAGTCAGAAGG96220.15061108467529866No Hit
CAACGGAATCCCAAAAGCAGCT95700.14979713992336396No Hit
TAGCAGCACGTAAATATTGGCGT94230.14749618072077939No Hit
AACATTCAACGCTGTCGGTGA93460.1462909163765684No Hit
CCCCCCACTGCTAAATTTGACTGGCT91810.1437082070675449No Hit
TGTAAACATCCTACACTCAGCT91780.14366124871647173No Hit
CCCCCCACTGCTAAATTTGACTGG90790.14211162313105763No Hit
CAAAGTGCTGTTCGTGCAGGT90280.141313331162814No Hit
AGCAGCATTGTACAGGGCTATGT89320.13981066392847305No Hit
CACTAGATTGTGAGCTCCTGGA89020.1393410804177415No Hit
ACTTGACTTGGAGTCAGAAGGC88610.1386993162864084No Hit
AGCAGCATTGTACAGGGCTATG88070.1378540659670916No Hit
CTCCCACTGCTTCACTTGACTAGCCT87960.13768188534649003No Hit
TGATGTAGTAGATTGTATAGTT86790.13585050965463696No Hit
TGTAAACATCCCCGACTGGAAGC86700.13570963460141752No Hit
TACCTGGTTGATCCTGCC86080.13473916201257233No Hit
CGCGACCTCAGATCAGACGTGG85510.13384695334218238No Hit
AGCTCGGTCTGAGGCCCCTCAGT82430.12902589596533848No Hit
TAGCTTATCAGACTGATGTTGACA81810.12805542337649328No Hit
CCTCGACACAAGGGTTTGT81740.1279458538906559No Hit
TGTCAGTTTGTCAAATACCCCA80880.1265997144932255No Hit
TTCACAGTGGCTAAGTTCCGC78980.12362568559192567No Hit
TCGTTCGCGCTTTCCCCTG78450.12279608805629993No Hit
ACTGGACTTGGAGTCAGAAGGCT77190.12082383731122744No Hit
TGAGGTAGTAGGTTGTATAGT75900.11880462821508178No Hit
AACGGAATCCCAAAAGCAGCTG75720.11852287810864284No Hit
ACTGGACTTGGAGTCAGAAGGCA75400.11802198903052918No Hit
TTCACAGTGGCTAAGTTCTG74720.11695759973953768No Hit
GTCAGGATGGCCGAGCGGTCTAAGG74690.11691064138846452No Hit
TTCACAGTGGCTAAGTTCTGC74440.11651932179618824No Hit
TGCAAGTAATCCAGGATAGGCT74150.11606539106914773No Hit
CAAAGAATTCTCCTTTTGGGCTT72530.11352964011119737No Hit
AACATTCAACGCTGTCGGTGAGA70280.11000776378071075No Hit
TCGTTTCCCGGCCAATGCACCA69480.10875554108542662No Hit
ATCCCACCGCTGCCACCA68820.10772245736181722No Hit
AACCGGGCGGAAACACCA66900.10471712289313533No Hit
TGAGGTAGTAGGTTGTGTGGTT66180.1035901224673796No Hit
CAAAGAATTCTCCTTTTGGGCT66070.10341794184677802No Hit
GGAATACCGGGTGCTGTAGGCTTT66060.10340228906308697No Hit
TAGCAGCACATAATGGTTTGT65390.10235355255578651No Hit
CGCGACCTCAGATCAGACGTGGCG65080.10186831626136392No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)