FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947210

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947210
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12259506
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTGTCTGAGCGTCGCT131768010.748230801469488No Hit
CAACGGAATCCCAAAAGCAGCTG10955048.935955494454669No Hit
CCCCCCACTGCTAAATTTGACTG8614647.02690630438127No Hit
TAGCAGCACGTAAATATTGGCG4823403.9344162807212624No Hit
TTCAAGTAATCCAGGATAGGCT4455763.634534703111202No Hit
AAGCTGCCAGTTGAAGAACTGT3882333.1667915493495418No Hit
AACATTCAACGCTGTCGGTGAGT3391752.766628606405511No Hit
CCCCCACTGCTAAATTTGACTG3291232.684635090516698No Hit
AACATTCAACGCTGTCGGTGAG2510652.047921017372152No Hit
TAGCTTATCAGACTGATGTTGAC1751551.4287280417334922No Hit
TGAGGTAGTAGGTTGTATAGTT1572541.2827107389155812No Hit
TATTGCACTTGTCCCGGCCTGTAT1420251.1584887678182139No Hit
CATAAAGTAGAAAGCACTACT1414611.15388825618259No Hit
TAGCTTATCAGACTGATGTTGA1357211.1070674462739363No Hit
TATTGCACTTGTCCCGGCCTGT1352341.1030950186736725No Hit
TAGCTTATCAGACTGATGTTGACT1344661.096830492191121No Hit
TATTGCACTTGTCCCGGCCTGTAA1335641.0894729363483324No Hit
TGAGGTAGTAGATTGTATAGTT955440.7793462477199327No Hit
AACCGGGCGGAAACACCA930940.7593617556857511No Hit
CAACGGAATCCCAAAAGCAGCT918470.7491900570871289No Hit
TTCAAGTAATTCAGGATAGGTT901610.7354374637934025No Hit
TGTAAACATCCTTGACTGGAAGCT839390.6846850109621057No Hit
TATTGCACTTGTCCCGGCCTGTA751820.6132547265770741No Hit
CAACGGAATCCCAAAAGCAGCTGT703400.5737588447691122No Hit
AGCAGCATTGTACAGGGCTATGA657080.535975919421223No Hit
CATTGCACTTGTCTCGGTCTGA600870.49012578483994385No Hit
CCCATAAAGTAGAAAGCACTAC561800.45825663774706743No Hit
CCCATAAAGTAGAAAGCACT554170.4520328959421367No Hit
ACTGGACTTGGAGTCAGAAGGC546030.4453931504254739No Hit
TCGTACGACTCTTAGCGG543750.44353336912596636No Hit
TTCTCACTACTGCACTTGACTAG476690.38883295950097824No Hit
TGTAAACATCCCCGACTGGAAGCT459310.3746562055599957No Hit
CAACACCAGTCGATGGGCTGT428980.34991622011523144No Hit
TGAGGTAGTAGTTTGTACAGTT399390.3257798478992547No Hit
CTTCTCACTACTGCACTTGACTAG378830.30900918846159053No Hit
TGTAAACATCCTACACTCTCAGCT375040.30591770989793554No Hit
TCGTTTCCCGGCCAATGCACCA363910.29683904065955025No Hit
CGCCTGTCTGAGCGTCGCT362330.29555024484673365No Hit
TAGCAGCACGTAAATATTGGC325460.26547562356917154No Hit
CTCCCACTGCTTCACTTGACTAG315270.2571637062700569No Hit
TGAGGTAGTAGTTTGTGCTGTT282230.23021319129824647No Hit
AACGGAATCCCAAAAGCAGCTG280350.22867968741970518No Hit
TCCTGTCTGAGCGTCGCT256030.20884202022495849No Hit
CACTAGATTGTGAGCTCCTGGA250040.2039560158459892No Hit
TGATGTAGTAGGTTGTATAGTT227090.18523584881805188No Hit
TTCACAGTGGCTAAGTTCCGC219950.1794117968538047No Hit
AGCTCGGTCTGAGGCCCCTCAGT216630.17670369425978502No Hit
TAGCTTATCAGACTGATGTTGACA216230.1763774168388188No Hit
CAACGGAATCCCAAAAGCAGCTGA214400.1748846976378983No Hit
CTACGCCTGTCTGAGCGTCGCT213290.17397927779471703No Hit
TATTGCACTTGTCCCGGCCTGTT212550.17337566456592948No Hit
TTCAAGTAATCCAGGATAGGC212020.17294334698314923No Hit
CCCCCCACTGCTAAATTTGACTGG206890.16875883905925734No Hit
AACATTCAACGCTGTCGGTGAGTT206700.16860385728429841No Hit
TTCACAGTGGCTAAGTTCTGC197560.16114841821522008No Hit
CAAAGAATTCTCCTTTTGGGCT197480.16108316273102685No Hit
CCCCCACTGCTAAATTTGACTGGCTT195240.15925600917361596No Hit
CCCATAAAGTAGAAAGCACTA195200.15922338143151935No Hit
TTCAAGTAATCCAGGATAGGCTT192250.1568170854518934No Hit
GGAATACCGGGTGCTGTAGGCTTT192110.15670288835455523No Hit
ATCCCGGACGAGCCCCCA185370.1512051138112743No Hit
TATTGCACTTGTCCCGGCCTGTAC184880.15080542397059066No Hit
TTCACAGTGGCTAAGTTCCG183220.1494513726735808No Hit
ACCCCCCACTGCTAAATTTGACTG181070.14769763153588733No Hit
TGTAAACATCCCCGACTGGAAGC177910.14512003991025413No Hit
CACTAGATTGTGAGCTCCTGGAA176070.14361916377380948No Hit
CCCCCCACTGCTAAATTTGACTGGCT166490.13580481954166831No Hit
GGAATACCGGGTGCTGTAGGCTT163600.13344746517518732No Hit
TGAGAACTGAATTCCATGGGTT161370.1316284685533006No Hit
TATTGCACTTGTCCCGGCCTGTAG160820.13117983709947204No Hit
TATTGCACTTGTCCCGGCCTGA159930.13045386983782217No Hit
ACCGGGTGCTGTAGGCTT157750.12867565789355623No Hit
TGAGGTAGTAGGTTGTGTGGTT157190.1282188695042035No Hit
AATGACACGATCACTCCCGTTG156970.1280394169226721No Hit
CAAAGAATTCTCCTTTTGGGCTT155840.1271176832084425No Hit
TTATAAAGCAATGAGACTGATT155590.1269137598203386No Hit
AATGCACCCGGGCAAGGATTCT151070.12322682496342023No Hit
CACCCGGCTGTGTGCACATGTGC148170.12086131366141506No Hit
AGCAGCATTGTACAGGGCTATGT146480.11948279155783277No Hit
TGTAAACATCCTACACTCAGCT144110.11754959783860784No Hit
TCAGTGCACTACAGAACTTTGT142370.11613029105740474No Hit
TAGCAGCACGTAAATATTGGCGT141240.11520855734317517No Hit
ACCACAGGGTAGAACCACGGAC139040.1134140315278609No Hit
ACCGGGTGCTGTAGGCTTT136910.11167660426121574No Hit
TGAGGTAGTAGGTTGTATAGT136220.111113775710049No Hit
CAAAGTGCTGTTCGTGCAGGT136050.11097510780613835No Hit
TGATGTAGTAGATTGTATAGTT135890.11084459683775186No Hit
TACGCCTGTCTGAGCGTCGCT130680.10659483342966673No Hit
TGTCAGTTTGTCAAATACCCCAA129610.1057220413285821No Hit
GCCTGTCTGAGCGTCGCTT126240.10297315405694162No Hit
AGCAGCATTGTACAGGGCTATG126100.10285895695960343No Hit
TGTCAGTTTGTCAAATACCCCA125060.10201063566509123No Hit
ATCCCACCGCTGCCACCA124650.10167620130860085No Hit
ACTGGACTTGGAGTCAGAAGG123970.10112152969295826No Hit
TGAGAACTGAATTCCATAGGCTGT123680.10088497856275776No Hit
ACTGGACTTGGAGTCAGAAGGCT123380.10064027049703309No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)