FastQCFastQC Report
Sun 4 Sep 2022
EGAF00003947211

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947211
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5541071
Sequences flagged as poor quality0
Sequence length18-26
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG4685118.45524267781445No Hit
CCCCCCACTGCTAAATTTGACTG4489758.10267545750632No Hit
TAGCAGCACGTAAATATTGGCG2076363.747217821247914No Hit
TTCAAGTAATCCAGGATAGGCT1952753.5241382036072086No Hit
TAGCTTATCAGACTGATGTTGAC1804333.2562838483751606No Hit
AAGCTGCCAGTTGAAGAACTGT1633382.947769483552909No Hit
TAGCTTATCAGACTGATGTTGACT1475362.6625899577897485No Hit
CCCCCACTGCTAAATTTGACTG1385292.5000401546921163No Hit
AACATTCAACGCTGTCGGTGAGT1319042.380478430974806No Hit
TATTGCACTTGTCCCGGCCTGTAA927101.6731422499368807No Hit
AACATTCAACGCTGTCGGTGAG920691.6615740891968358No Hit
TGTAAACATCCTTGACTGGAAGCT901401.6267613246608823No Hit
TATTGCACTTGTCCCGGCCTGTAT774641.3979968854396558No Hit
TAGCTTATCAGACTGATGTTGA698671.2608934265595946No Hit
GCCTGTCTGAGCGTCGCT581231.0489488403956564No Hit
TGAGGTAGTAGGTTGTATAGTT550550.9935804829066438No Hit
TGAGGTAGTAGATTGTATAGTT411140.7419865221001499No Hit
TAGCTTATCAGACTGATGTTGACA410020.7399652522048535No Hit
CATAAAGTAGAAAGCACTACT405250.7313568080972073No Hit
TGTAAACATCCCCGACTGGAAGCT395630.713995543460822No Hit
AGCAGCATTGTACAGGGCTATGA391190.7059826520901826No Hit
TTCAAGTAATTCAGGATAGGTT383710.6924834567180244No Hit
TATTGCACTTGTCCCGGCCTGT344860.62237065722493No Hit
TTAATGCTAATCGTGATAGGGGTT343950.6207283754350017No Hit
CAACGGAATCCCAAAAGCAGCTGT339100.6119755549062628No Hit
TATTGCACTTGTCCCGGCCTGTA313290.5653961120512623No Hit
ACTGGACTTGGAGTCAGAAGGC305000.5504351054155415No Hit
TGTAAACATCCTACACTCTCAGCT287920.5196107395122712No Hit
CAAAGAATTCTCCTTTTGGGCT272180.4912046786623019No Hit
CCCATAAAGTAGAAAGCACTAC255470.46104805370658486No Hit
TCCTGTCTGAGCGTCGCT231340.41750051569452906No Hit
CAACGGAATCCCAAAAGCAGCT224660.40544508453329686No Hit
CCCCCACTGCTAAATTTGACTGGCTT223640.4036042851643663No Hit
TTCTCACTACTGCACTTGACTAG196630.35485919599297683No Hit
AACCGGGCGGAAACACCA179340.3236558419843384No Hit
TAGCAGCACGTAAATATTGGC176690.318873373035646No Hit
CAAAGAATTCTCCTTTTGGGCTT172720.3117086931389257No Hit
CATTGCACTTGTCTCGGTCTGA157030.2833928675521393No Hit
TGAGGTAGTAGTTTGTACAGTT155840.28124526828838686No Hit
CTTCTCACTACTGCACTTGACTAG145190.26202515723043435No Hit
TGAGAACTGAATTCCATAGGCTGT135030.24368935175167397No Hit
CCCATAAAGTAGAAAGCACT134550.24282309322511839No Hit
TGTCAGTTTGTCAAATACCCCA124650.22495651111490902No Hit
TGAGAACTGAATTCCATGGGTT123030.22203288858778383No Hit
GGAATACCGGGTGCTGTAGGCTTT122630.22131100648232083No Hit
TGTCAGTTTGTCAAATACCCCAA121530.21932583069229758No Hit
TTCAAGTAATCCAGGATAGGCTT118780.2143628912172394No Hit
CCCCCCACTGCTAAATTTGACTGGCT113090.20409411826702817No Hit
ACCCCCCACTGCTAAATTTGACTG112850.20366098900375038No Hit
TCGTTTCCCGGCCAATGCACCA109560.19772350868631716No Hit
TATTGCACTTGTCCCGGCCTGTAAT108230.19532325068565265No Hit
AACATTCAACGCTGTCGGTGAGTT104160.18797810026256656No Hit
CACTAGATTGTGAGCTCCTGGAA102240.18451306615634414No Hit
TGTAAACATCCCCGACTGGAAGC101450.1830873489980547No Hit
ATCACATTGCCAGGGATTTCCA101350.18290687847168896No Hit
TATTGCACTTGTCCCGGCCTGTAAA101160.18256398447159403No Hit
AACGGAATCCCAAAAGCAGCTG100530.1814270201554898No Hit
TGAGGTAGTAGTTTGTGCTGTT99250.17911699741800818No Hit
TATTGCACTTGTCCCGGCCTGTAG97310.1756158692065126No Hit
CCCCCCACTGCTAAATTTGACTGG96270.1737389757323088No Hit
ATCACATTGCCAGGGATTTCC95340.17206059983710728No Hit
AGCTCGGTCTGAGGCCCCTCAGT86760.15657622867492585No Hit
TCGTACGACTCTTAGCGG85680.15462714699017574No Hit
ATGCACCTGGGCAAGGATTCTGA85390.15410378246371506No Hit
CAACGGAATCCCAAAAGCAGCTGA83210.15016952498894165No Hit
CTCCCACTGCTTCACTTGACTAG82920.149646160462481No Hit
CAGTGCAATAGTATTGTCAAAGC82780.14939350172556892No Hit
TAGCAGCACGTAAATATTGGCGT81300.14672253793535583No Hit
TTATAAAGCAATGAGACTGATT80500.1452787737244298No Hit
CACTAGATTGTGAGCTCCTGGA77250.13941348161754288No Hit
TTCACAGTGGCTAAGTTCTGC75900.13697712951160526No Hit
TTCACAGTGGCTAAGTTCCGC75680.1365800943536006No Hit
TGTAAACATCCTACACTCAGCT74840.1350641419321283No Hit
CCCCCCACTGCTAAATTTGACTGTA74700.13481148319521624No Hit
TTCAAGTAATCCAGGATAGGC74480.1344144480372116No Hit
AGCAGCATTGTACAGGGCTATGT73880.13333162487901706No Hit
TAGCAGCACATCATGGTTTACA72920.13159910782590586No Hit
TATTGCACTTGTCCCGGCCTGTT71350.12876572056196356No Hit
TGTGCAAATCCATGCAAAACTG71000.12813407371968344No Hit
GGAATACCGGGTGCTGTAGGCTT70270.12681663887721345No Hit
CTCCCACTGCTTCACTTGACTAGCCT69750.12587819214011153No Hit
TGTAAACATCCTTGACTGGAAGC68900.12434419266600266No Hit
TGTCAGTTTGTCAAATACCCC67960.12264776971816459No Hit
CGCCTGTCTGAGCGTCGCT67090.12107767613878256No Hit
ACTGGACTTGGAGTCAGAAGGCT66750.120464076349139No Hit
AATGCACCCGGGCAAGGATTCT65460.11813600655902082No Hit
TAGCTTATCAGACTGATGTTGACC65350.11793748898001849No Hit
CACCCGTAGAACCGACCTTGCG64980.11726974803246522No Hit
CAACACCAGTCGATGGGCTGT64470.11634934834799987No Hit
TTTAAACATCCTTGACTGGAAGCT64150.11577184266362947No Hit
CAAAGAATTCTCCTTTTGGGC63820.11517628992662249No Hit
ACTGGACTTGGAGTCAGAAGGCA62930.11357010224196729No Hit
TATTGCACTTGTCCCGGCCTGTTT61140.11033967982002037No Hit
CTACGGGGATGATTTTACGAACTGAA60810.10974412708301337No Hit
TTCACAGTGGCTAAGTTCCG60350.10891396266173091No Hit
CTACGCCTGTCTGAGCGTCGCT59780.10788528066144613No Hit
TCAGTGCACTACAGAACTTTGT58450.10548502266078165No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)