FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947212

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947212
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9402369
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG6432046.8408716994621255No Hit
CCCCCCACTGCTAAATTTGACTG5414825.758995419133199No Hit
AAGCTGCCAGTTGAAGAACTGT4143584.40695318381995No Hit
TAGCAGCACGTAAATATTGGCG3518943.7426099741458776No Hit
TTCAAGTAATCCAGGATAGGCT3112893.310750726758331No Hit
AACATTCAACGCTGTCGGTGAGT2897013.0811490168062963No Hit
GCCTGTCTGAGCGTCGCT2779272.95592525670924No Hit
TAGCTTATCAGACTGATGTTGAC1892582.0128756912220735No Hit
CCCCCACTGCTAAATTTGACTG1777161.8901193943781616No Hit
AACATTCAACGCTGTCGGTGAG1546291.6445748938379252No Hit
TAGCTTATCAGACTGATGTTGACT1359901.4463376198062423No Hit
TATTGCACTTGTCCCGGCCTGTAA1354641.4407432850167867No Hit
TATTGCACTTGTCCCGGCCTGTAT1233631.3120416780068938No Hit
TATTGCACTTGTCCCGGCCTGT1112031.1827125695662444No Hit
TGTAAACATCCTTGACTGGAAGCT1085961.154985514820786No Hit
TGAGGTAGTAGGTTGTATAGTT1009781.0739633809309121No Hit
TAGCTTATCAGACTGATGTTGA990761.0537344365021197No Hit
TTAATGCTAATCGTGATAGGGGTT921040.9795829114981555No Hit
AGCAGCATTGTACAGGGCTATGA840050.893445045604996No Hit
CATAAAGTAGAAAGCACTACT750250.7979372007203717No Hit
TTCAAGTAATTCAGGATAGGTT706870.7517998921335676No Hit
CGCCTGTCTGAGCGTCGCT697840.7421959295577529No Hit
TGAGGTAGTAGATTGTATAGTT670550.7131713294809No Hit
AACCGGGCGGAAACACCA642370.6832001594491771No Hit
TATTGCACTTGTCCCGGCCTGTA614560.6536225072638608No Hit
TCGTACGACTCTTAGCGG611830.6507189836944285No Hit
ACTGGACTTGGAGTCAGAAGGC564070.5999232746555682No Hit
CCCATAAAGTAGAAAGCACTAC526290.5597419118522151No Hit
TGTAAACATCCCCGACTGGAAGCT523700.556987286927369No Hit
TGTAAACATCCTACACTCTCAGCT509830.5422356854958575No Hit
CAACGGAATCCCAAAAGCAGCTGT480450.5109882413676808No Hit
CCCATAAAGTAGAAAGCACT337130.35855857178121814No Hit
CATTGCACTTGTCTCGGTCTGA336100.357463103181762No Hit
TGAGGTAGTAGTTTGTACAGTT316050.33613869015351344No Hit
TTCTCACTACTGCACTTGACTAG310370.3300976594302989No Hit
TACGCCTGTCTGAGCGTCGCT292600.31119816718531257No Hit
CTACGCCTGTCTGAGCGTCGCT289870.3082946436158802No Hit
TAGCTTATCAGACTGATGTTGACA274630.29208596259091724No Hit
AGCTCGGTCTGAGGCCCCTCAGT273040.29039489941311597No Hit
AACATTCAACGCTGTCGGTGAGTT243110.25856249632406475No Hit
TTCAAGTAATCCAGGATAGGCTT210710.22410309571981274No Hit
TGAGGTAGTAGTTTGTGCTGTT210460.22383720528305154No Hit
TGTAAACATCCTACACTCAGCT205010.2180407937616573No Hit
ACGCCTGTCTGAGCGTCGCT203350.21627528126156292No Hit
GGAATACCGGGTGCTGTAGGCTTT201720.21454167561387985No Hit
TCCTGTCTGAGCGTCGCT189680.20173639217946032No Hit
TGTCAGTTTGTCAAATACCCCAA187470.19938592071849126No Hit
GCGTTCGCGCTTTCCCCTG185620.19741833148645838No Hit
CTTCTCACTACTGCACTTGACTAG185130.1968971862304064No Hit
CAAAGAATTCTCCTTTTGGGCT183590.19525930113995738No Hit
AGCAGCATTGTACAGGGCTATGT177780.18908000738962702No Hit
TATTGCACTTGTCCCGGCCTGTT172770.1837515630369325No Hit
TAGCAGCACGTAAATATTGGC172480.1834431301302895No Hit
TGTCAGTTTGTCAAATACCCCA168320.1790187132625831No Hit
CAACGGAATCCCAAAAGCAGCT167340.17797642275047915No Hit
CTCCCACTGCTTCACTTGACTAG167130.17775307478359975No Hit
CAACACCAGTCGATGGGCTGT165860.17640235136485286No Hit
TGTAAACATCCCCGACTGGAAGC161480.17174395091279657No Hit
TGAGAACTGAATTCCATGGGTT160230.17041449872899053No Hit
TTCACAGTGGCTAAGTTCCG157720.16774495874390805No Hit
CAAAGAATTCTCCTTTTGGGCTT157300.16729826281014923No Hit
TTATAAAGCAATGAGACTGATT153920.16370342410513775No Hit
AACGGAATCCCAAAAGCAGCTG150810.16039574707182838No Hit
TTCAAGTAATCCAGGATAGGC150600.160172399104949No Hit
TATTGCACTTGTCCCGGCCTGTAG150580.16015112787000807No Hit
CCCCCACTGCTAAATTTGACTGGCTT150310.15986396619830598No Hit
ATCCCGGACGAGCCCCCA146970.1563116699631763No Hit
CCCCCCACTGCTAAATTTGACTGG146040.1553225575384246No Hit
TCGTTTCCCGGGCGGCGCACCA144900.15411009714679352No Hit
CCCCCCACTGCTAAATTTGACTGGCT143540.1526636531708126No Hit
TCGTTTCCCGGCCAATGCACCA143410.15252539014369676No Hit
GGAATACCGGGTGCTGTAGGCTT142120.15115339549000895No Hit
CAAAGTGCTGTTCGTGCAGGTAG141790.15080242011348416No Hit
TGAGAACTGAATTCCATAGGCTGT135960.14460185512821289No Hit
ACCACAGGGTAGAACCACGGAC133630.1421237562575985No Hit
ACGCGACCTCAGATCAGA131110.13944358065504556No Hit
TGAGGTAGTAGGTTGTGTGGTT129580.137816331182067No Hit
CACTAGATTGTGAGCTCCTGGA128650.13682721875731532No Hit
TAGCAGCACGTAAATATTGGCGT128230.13638052282355648No Hit
AGCAGCATTGTACAGGGCTATG124900.13283886220589725No Hit
CACTAGATTGTGAGCTCCTGGAA121510.12923338788341535No Hit
CCCCCCACTGCTAAATTTGACTGTA119740.12735088359114602No Hit
TGATGTAGTAGGTTGTATAGTT112150.11927844993107589No Hit
ACCCCCCACTGCTAAATTTGACTG111320.11839569368102867No Hit
CAAAGTGCTGTTCGTGCAGGT111160.11822552380150152No Hit
TGTCAGTTTGTCAAATACCCC110950.1180021758346221No Hit
AGAGGTAGTAGGTTGCATAGTT110410.1174278524912179No Hit
ACCGGGTGCTGTAGGCTT110290.11730022508157252No Hit
TTCACAGTGGCTAAGTTCTGC107270.11408826860549719No Hit
TGTAAACATCCTTGACTGGAAGC106370.11313106303315686No Hit
TATTGCACTTGTCCCGGCCTGTTT105090.1117697039969395No Hit
AATGACACGATCACTCCCGTTG104480.11112093133124215No Hit
TTCACAGTGGCTAAGTTCCGC103210.10977020791249524No Hit
CACCCGGCTGTGTGCACATGTGC102050.10853647628592325No Hit
GGGCTACGCCTGTCTGAGCGTCGCT101060.1074835501563489No Hit
ACCGGGTGCTGTAGGCTTT100580.10697304051776738No Hit
TTAATGCTAATCGTGATAGGGGTTT99820.10616473359001333No Hit
GACCCGCGGGCGCTCTCCAGTCCT99250.10555850339419778No Hit
ATCACATTGCCAGGGATTTCC99060.10535642666225926No Hit
ATCACATTGCCAGGGATTTCCA98630.10489909511103No Hit
ACCGGGTGCTGTAGGCTTA98410.10466511152668014No Hit
CCCCCCACTGCTAAATTTGACTGT97310.10349519360493084No Hit
TGAGGTAGTAGGTTGTATAGT96540.10267625105970635No Hit
TATTGCACTTGTCCCGGCCTGTAAT96420.10254862365006095No Hit
ACTGGACTTGGAGTCAGAAGGCT94750.10077247553249612No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)