FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947213

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947213
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6076617
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG65689010.810126753093046No Hit
CCCCCCACTGCTAAATTTGACTG4679017.700024536678879No Hit
TAGCAGCACGTAAATATTGGCG2484034.0878501969105505No Hit
TTCAAGTAATCCAGGATAGGCT2216313.647276107742186No Hit
AAGCTGCCAGTTGAAGAACTGT1665022.740044337169843No Hit
CCCCCACTGCTAAATTTGACTG1268452.08742792247726No Hit
AACATTCAACGCTGTCGGTGAGT1245302.0493310669407006No Hit
TATTGCACTTGTCCCGGCCTGTAA1138941.8742994662984354No Hit
AACATTCAACGCTGTCGGTGAG1124661.8507995484987783No Hit
TATTGCACTTGTCCCGGCCTGTAT993301.6346266351820429No Hit
GGAATACCGGGTGCTGTAGGCTTT959271.5786250803695543No Hit
TTAATGCTAATCGTGATAGGGGTT789521.2992755673099028No Hit
GCCTGTCTGAGCGTCGCT767651.2632851469822763No Hit
TAGCTTATCAGACTGATGTTGACT749441.2333178148301924No Hit
TAGCTTATCAGACTGATGTTGAC721611.1875193055609725No Hit
GGAATACCGGGTGCTGTAGGCTT589460.970046326763724No Hit
TGTAAACATCCTTGACTGGAAGCT556190.9152954678565393No Hit
CAACGGAATCCCAAAAGCAGCTGT513320.8447463448823581No Hit
TGAGGTAGTAGGTTGTATAGTT458720.7548937180013156No Hit
TAGCTTATCAGACTGATGTTGA445580.733269844059614No Hit
TATTGCACTTGTCCCGGCCTGTA434990.7158423840107087No Hit
TATTGCACTTGTCCCGGCCTGT422500.6952881841985434No Hit
CATAAAGTAGAAAGCACTACT382090.6287873663915301No Hit
CCCATAAAGTAGAAAGCACTAC360030.5924842720875777No Hit
TTCAAGTAATTCAGGATAGGTT346160.5696590718157817No Hit
TGAATACCGGGTGCTGTAGGCTTT317130.521885779538187No Hit
TGAGAACTGAATTCCATGGGTT315460.5191375398515325No Hit
CAAAGAATTCTCCTTTTGGGCT297350.48933477295014643No Hit
TGTAAACATCCCCGACTGGAAGCT294130.4840357718776747No Hit
AGCAGCATTGTACAGGGCTATGA293080.48230783674534694No Hit
TGAGGTAGTAGATTGTATAGTT275600.4535418309233575No Hit
CCCCCACTGCTAAATTTGACTGGCTT265030.43614728392459157No Hit
AACCGGGCGGAAACACCA247750.4077104086039979No Hit
TCCTGTCTGAGCGTCGCT239210.393656536194399No Hit
TTCTCACTACTGCACTTGACTAG234790.38638275211355266No Hit
TGTAAACATCCTACACTCTCAGCT225880.37171998827637154No Hit
TGAATACCGGGTGCTGTAGGCTT193660.3186970645015146No Hit
ACTGGACTTGGAGTCAGAAGGC187560.3086585842089439No Hit
TAGCAGCACGTAAATATTGGC184000.30280006128409936No Hit
TAGCTTATCAGACTGATGTTGACA175640.28904240632575656No Hit
CAACGGAATCCCAAAAGCAGCT175480.28877910192463996No Hit
TGAGAACTGAATTCCATAGGCTGT173960.28627771011403225No Hit
CTTCTCACTACTGCACTTGACTAG171840.2827889267992372No Hit
GGAATACCGGGTGCTGTAGGCTTA164850.2712858157754553No Hit
ACCCCCCACTGCTAAATTTGACTG155280.2555369212836682No Hit
TCGTACGACTCTTAGCGG153730.25298615989785106No Hit
CATTGCACTTGTCTCGGTCTGA148800.24487309303844557No Hit
TCGTTTCCCGGCCAATGCACCA143130.23554224332387577No Hit
AGCTCGGTCTGAGGCCCCTCAGT141570.23297502541298884No Hit
TGATGTAGTAGGTTGTATAGTT136990.22543793693102593No Hit
CCCCCCACTGCTAAATTTGACTGGCT133850.22027058805911248No Hit
TATTGCACTTGTCCCGGCCTGTAG133760.2201224793334844No Hit
CACTAGATTGTGAGCTCCTGGAA126180.20764843333058508No Hit
CCCATAAAGTAGAAAGCACT126140.20758260723030597No Hit
TTCACAGTGGCTAAGTTCCG125310.20621671564951352No Hit
CAAAGAATTCTCCTTTTGGGCTT124890.20552554159658246No Hit
CTCCCACTGCTTCACTTGACTAG121670.2002265405241107No Hit
TCGTTTCCCGGGCGGCGCACCA120010.19749475736252586No Hit
TATTGCACTTGTCCCGGCCTGTAAA118260.19461486547531298No Hit
CCCCCCACTGCTAAATTTGACTGG115960.19082986470926175No Hit
TGAGGTAGTAGTTTGTACAGTT107040.17615064434701083No Hit
CACTAGATTGTGAGCTCCTGGA105810.1741264917634269No Hit
CCCATAAAGTAGAAAGCACTA105110.17297453500854176No Hit
TATTGCACTTGTCCCGGCCTGTAAT104000.1711478607257953No Hit
TTCAAGTAATCCAGGATAGGC103170.16978196914500288No Hit
AACATTCAACGCTGTCGGTGAGTT102340.16841607756421048No Hit
TATTGCACTTGTCCCGGCCTGTT99690.16405509842071667No Hit
AACGGAATCCCAAAAGCAGCTG99470.16369305486918134No Hit
TTAATGCTAATCGTGATAGGGGTTT98610.16227779371317955No Hit
GGAATACCGGGTGCTGTAGGCT98250.16168535881066717No Hit
TTAATGCTAATCGTGATAGGGGT96240.1583775972716398No Hit
CTCGTACGACTCTTAGCGG95630.15737374924238273No Hit
CTACGCCTGTCTGAGCGTCGCT91080.14988603033562917No Hit
ATCACATTGCCAGGGATTTCC90470.14888218230637212No Hit
TGATAACTGAATTCCATGGGTT88820.1461668556698571No Hit
TGTAAACATCCCCGACTGGAAGC86180.14182233305143307No Hit
ATCACATTGCCAGGGATTTCCA84690.13937031081603463No Hit
TGATGTAGTAGATTGTATAGTT82660.13602963622686767No Hit
GGGAATACCGGGTGCTGTAGGCTTT80700.13280415731318923No Hit
TGTCAGTTTGTCAAATACCCCAA80210.13199778758476963No Hit
TATTGCACTTGTCCCGGCCTGTTT79980.13161928750816448No Hit
TGAGGTAGTAGTTTGTGCTGTT79640.1310597656557917No Hit
TGAGAACTGAATTCCATGGGTTG78400.12901915654713797No Hit
TGTCAGTTTGTCAAATACCCCA77860.12813050419336944No Hit
TTCAAGTAATCCAGGATAGGCTT75770.12469109045378374No Hit
CCCCCCACTGCTAAATTTGACTGTA73970.1217289159412219No Hit
ATGCACCTGGGCAAGGATTCTGA73810.1214656115401053No Hit
TAGCAGCACATCATGGTTTACA73190.12044530698577843No Hit
CAACGGAATCCCAAAAGCAGCTGA67770.11152587039795334No Hit
ACTGGACTTGGAGTCAGAAGGCA67270.11070304414446393No Hit
CCCCCCACTGCTAAATTTGACTGT66940.11015997881716093No Hit
CAACACCAGTCGATGGGCTGT65820.10831684800934469No Hit
CACCCGGCTGTGTGCACATGTGC65420.10765858700655315No Hit
TAGCAGCACGTAAATATTGGCGT65070.10708260862911058No Hit
CCCCCACTGCTAAATTTGACTGGCTA64050.10540404307199219No Hit
ATCCCGGACGAGCCCCCA63800.10499262994524751No Hit
TTCACAGTGGCTAAGTTCTGC63300.1041698036917581No Hit
ACTTGACTTGGAGTCAGAAGGC62740.10324823828784997No Hit
TTCACAGTGGCTAAGTTCCGC61290.1008620421527307No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)