FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947214

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947214
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7908720
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG7672179.700899766333869No Hit
CCCCCCACTGCTAAATTTGACTG7573189.575734126382018No Hit
TAGCAGCACGTAAATATTGGCG2724183.4445270536825174No Hit
TTCAAGTAATCCAGGATAGGCT2650543.351414641054431No Hit
AACATTCAACGCTGTCGGTGAGT2493943.1534053551017105No Hit
CCCCCACTGCTAAATTTGACTG2284942.8891400884087437No Hit
AAGCTGCCAGTTGAAGAACTGT2104652.6611765241404424No Hit
AACATTCAACGCTGTCGGTGAG1871452.3663121213040794No Hit
TAGCTTATCAGACTGATGTTGAC1720252.175130741763522No Hit
TAGCTTATCAGACTGATGTTGACT1664262.1043354676863006No Hit
TATTGCACTTGTCCCGGCCTGTAA1394131.7627757715534247No Hit
TATTGCACTTGTCCCGGCCTGTAT1105031.3972298930800433No Hit
GCCTGTCTGAGCGTCGCT991551.2537427042555558No Hit
TAGCTTATCAGACTGATGTTGA917371.159947500986253No Hit
TGAGGTAGTAGGTTGTATAGTT892911.1290196137933826No Hit
TGTAAACATCCTTGACTGGAAGCT891871.1277046095954846No Hit
TGAGGTAGTAGATTGTATAGTT588410.7440015577742037No Hit
CATAAAGTAGAAAGCACTACT550290.6958015962127879No Hit
AGCAGCATTGTACAGGGCTATGA544650.688670227293418No Hit
TATTGCACTTGTCCCGGCCTGTA505500.6391679058052377No Hit
TGTAAACATCCCCGACTGGAAGCT468780.5927381422025308No Hit
CAACGGAATCCCAAAAGCAGCTGT462690.5850377810821473No Hit
TATTGCACTTGTCCCGGCCTGT448350.566905896276515No Hit
TTCAAGTAATTCAGGATAGGTT424670.536964262232068No Hit
ACTGGACTTGGAGTCAGAAGGC419450.5303639527003106No Hit
TCCTGTCTGAGCGTCGCT415690.5256097067540638No Hit
TAGCTTATCAGACTGATGTTGACA370380.4683185142475647No Hit
CCCATAAAGTAGAAAGCACTAC366020.4628056120333No Hit
CCCCCACTGCTAAATTTGACTGGCTT353690.4472152257255283No Hit
CAACGGAATCCCAAAAGCAGCT319180.4035798460433547No Hit
CAAAGAATTCTCCTTTTGGGCT297410.3760532677854318No Hit
TGTAAACATCCTACACTCTCAGCT275880.3488301520347161No Hit
TTCTCACTACTGCACTTGACTAG267880.3387147351278083No Hit
CTTCTCACTACTGCACTTGACTAG240020.3034877957495018No Hit
TCGTTTCCCGGCCAATGCACCA228700.28917448082622726No Hit
CCCATAAAGTAGAAAGCACT221960.28065224208215744No Hit
TAGCAGCACGTAAATATTGGC219240.27721300033380875No Hit
AACATTCAACGCTGTCGGTGAGTT218960.276858960742067No Hit
TGAGGTAGTAGTTTGTACAGTT218420.2761761701008507No Hit
CATTGCACTTGTCTCGGTCTGA189740.23991240048958618No Hit
ACCCCCCACTGCTAAATTTGACTG187410.23696628531544925No Hit
CCCCCCACTGCTAAATTTGACTGGCT177390.22429672563954722No Hit
GGAATACCGGGTGCTGTAGGCTTT160480.20291526315257083No Hit
CAAAGAATTCTCCTTTTGGGCTT159630.20184050010621188No Hit
AACGGAATCCCAAAAGCAGCTG156600.19800928595272055No Hit
CGCCTGTCTGAGCGTCGCT156140.19742764948057334No Hit
CCCCCCACTGCTAAATTTGACTGG149350.18884218938083533No Hit
TTCACAGTGGCTAAGTTCCG147020.18589607420669843No Hit
TATTGCACTTGTCCCGGCCTGTAG146970.18583285285103027No Hit
AACCGGGCGGAAACACCA142610.1803199506367655No Hit
CAACGGAATCCCAAAAGCAGCTGA142410.1800670652140928No Hit
CTCCCACTGCTTCACTTGACTAG141420.17881528237186298No Hit
TGAGGTAGTAGTTTGTGCTGTT140940.17820835735744847No Hit
ACTGGACTTGGAGTCAGAAGGCA140510.17766465369870219No Hit
CAACACCAGTCGATGGGCTGT139500.1763875823142051No Hit
TATTGCACTTGTCCCGGCCTGTAAA135860.17178506762156204No Hit
CACTAGATTGTGAGCTCCTGGAA135400.17120343114941483No Hit
TTCAAGTAATCCAGGATAGGCTT133600.16892746234536057No Hit
CTACGCCTGTCTGAGCGTCGCT128740.16278234657441407No Hit
TGTCAGTTTGTCAAATACCCCA128340.16227657572906867No Hit
TATTGCACTTGTCCCGGCCTGTAAT128180.16207426739093053No Hit
ACTGGACTTGGAGTCAGAAGGCT128050.16190989186619326No Hit
TGTAAACATCCCCGACTGGAAGC124450.15735795425808474No Hit
TGTCAGTTTGTCAAATACCCCAA123350.15596708443338492No Hit
AGCTCGGTCTGAGGCCCCTCAGT122300.15463943596435326No Hit
ATCACATTGCCAGGGATTTCC115990.14666090087902972No Hit
TAGCAGCACGTAAATATTGGCGT114700.14502978990279083No Hit
TCGTACGACTCTTAGCGG111750.1412997299183686No Hit
TTAATGCTAATCGTGATAGGGGTT110590.13983299446686695No Hit
TTCAAGTAATCCAGGATAGGC109220.13810072932155898No Hit
CCCCCCACTGCTAAATTTGACTGTA108710.1374558714937436No Hit
TATTGCACTTGTCCCGGCCTGTT108410.13707654335973457No Hit
CACTAGATTGTGAGCTCCTGGA107670.1361408672958456No Hit
ATGCACCTGGGCAAGGATTCTGA105860.13385225422065772No Hit
AGCAGCATTGTACAGGGCTATGT105720.13367523442478682No Hit
TTATAAAGCAATGAGACTGATT104590.13224643178668608No Hit
ATCACATTGCCAGGGATTTCCA99110.12531737120545422No Hit
GGAATACCGGGTGCTGTAGGCTT96320.12178961955917013No Hit
CCCATAAAGTAGAAAGCACTA93770.11856533042009328No Hit
TTCACAGTGGCTAAGTTCTGC92970.11755378872940249No Hit
TACGCCTGTCTGAGCGTCGCT92840.11738941320466523No Hit
CTCCCACTGCTTCACTTGACTAGCCT89290.11290069695222488No Hit
TAGCAGCACATCATGGTTTACA88410.11178800109246502No Hit
CACCCGGCTGTGTGCACATGTGC87730.11092819065537786No Hit
AATGCACCCGGGCAAGGATTCT87160.11020746720076069No Hit
ACTGGACTTGGAGTCAGAAGG87130.11016953438735977No Hit
CCCCCCACTGCTAAATTTGACTGT86950.10994193750695434No Hit
GGGAATACCGGGTGCTGTAGGCTTT84540.10689466816374837No Hit
TTCACAGTGGCTAAGTTCTG80700.10203926804843262No Hit
TGTAAACATCCTACACTCAGCT80440.10171051699895811No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)