FastQCFastQC Report
Fri 9 Sep 2022
EGAF00003947215

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947215
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7258089
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG6541069.012096710304874No Hit
TTCAAGTAATCCAGGATAGGCT3061004.217363551204731No Hit
CCCCCCACTGCTAAATTTGACTG2860323.94087203945832No Hit
AAGCTGCCAGTTGAAGAACTGT2380373.2796098256717436No Hit
TAGCAGCACGTAAATATTGGCG2234753.078978502468074No Hit
AACATTCAACGCTGTCGGTGAGT2177412.9999769911887277No Hit
AACATTCAACGCTGTCGGTGAG1673242.305345112191377No Hit
TAGCTTATCAGACTGATGTTGAC1616452.22710137613358No Hit
GCCTGTCTGAGCGTCGCT1565232.156531836410383No Hit
TAGCTTATCAGACTGATGTTGA1140761.5717084758811857No Hit
TAGCTTATCAGACTGATGTTGACT1096911.5112931241267502No Hit
TGAGGTAGTAGGTTGTATAGTT1021951.4080152502952223No Hit
TATTGCACTTGTCCCGGCCTGTAA990901.3652353946059355No Hit
TATTGCACTTGTCCCGGCCTGT952881.312852460199923No Hit
CCCCCACTGCTAAATTTGACTG926621.2766721378037662No Hit
TATTGCACTTGTCCCGGCCTGTAT867451.1951493016963557No Hit
TTCAAGTAATTCAGGATAGGTT727971.002977505511437No Hit
TGTAAACATCCTTGACTGGAAGCT662330.9125404772523457No Hit
TGAGGTAGTAGATTGTATAGTT656080.9039293951892846No Hit
CATAAAGTAGAAAGCACTACT643920.8871756739273934No Hit
TCGTACGACTCTTAGCGG590330.8133408118858834No Hit
TATTGCACTTGTCCCGGCCTGTA463210.6381982915888741No Hit
AGCAGCATTGTACAGGGCTATGA460530.6345058596002335No Hit
CAACGGAATCCCAAAAGCAGCT449380.619143689199733No Hit
CAACGGAATCCCAAAAGCAGCTGT409300.5639225421457357No Hit
TGTAAACATCCCCGACTGGAAGCT388370.5350857505329571No Hit
ACTGGACTTGGAGTCAGAAGGC382860.5274942205861626No Hit
CCCATAAAGTAGAAAGCACTAC345250.475676173163487No Hit
CATTGCACTTGTCTCGGTCTGA324010.44641227188038063No Hit
TCGTTTCCCGGCCAATGCACCA297510.40990128393300224No Hit
CACCCGGCTGTGTGCACATGTGC271060.37345918464212824No Hit
TGAGGTAGTAGTTTGTACAGTT266160.3667080963046884No Hit
CGCCTGTCTGAGCGTCGCT251040.3458761665777314No Hit
CCCCCACTGCTAAATTTGACTGGCTT247460.34094373877201006No Hit
TAGCTTATCAGACTGATGTTGACA238200.328185559587379No Hit
CTCCCACTGCTTCACTTGACTAG219000.30173231548965573No Hit
CAACACCAGTCGATGGGCTGT216430.2981914385453251No Hit
TTCTCACTACTGCACTTGACTAG212870.2932865662022056No Hit
CCCATAAAGTAGAAAGCACT202330.27876483741105956No Hit
AACCGGGCGGAAACACCA194120.26745332001302274No Hit
TGAGGTAGTAGTTTGTGCTGTT192450.26515243888577283No Hit
AACGGAATCCCAAAAGCAGCTG185750.2559213589141715No Hit
AGCTCGGTCTGAGGCCCCTCAGT184020.2535378113991162No Hit
ACCGGGTGCTGTAGGCTT172070.23707342249454366No Hit
TAGCAGCACGTAAATATTGGC170970.23555787205144493No Hit
TGTAAACATCCTACACTCTCAGCT166590.22952322574165182No Hit
TTCACAGTGGCTAAGTTCCGC158780.21876281759565086No Hit
GGAATACCGGGTGCTGTAGGCTTT158220.2179912646428006No Hit
CCCCCCACTGCTAAATTTGACTGGCT156110.2150841633383112No Hit
AATGCACCCGGGCAAGGATTCT151200.20831929726957055No Hit
TTCAAGTAATCCAGGATAGGCTT148670.20483353125044348No Hit
TATTGCACTTGTCCCGGCCTGTT146220.20145798708172358No Hit
CTACGCCTGTCTGAGCGTCGCT146210.20144420935042268No Hit
AACATTCAACGCTGTCGGTGAGTT144360.19889532905975663No Hit
TCCTGTCTGAGCGTCGCT144070.1984957748520306No Hit
TTCAAGTAATCCAGGATAGGC139000.19151046508247555No Hit
CACTAGATTGTGAGCTCCTGGA137900.18999491463937684No Hit
CAAAGAATTCTCCTTTTGGGCT137490.18943002765604006No Hit
TTCACAGTGGCTAAGTTCTGC137210.1890442511796149No Hit
TGAGGTAGTAGGTTGTGTGGTT132440.18247227334908678No Hit
ACCGGGTGCTGTAGGCTTT130160.17933095061248217No Hit
CTCCCACTGCTTCACTTGACTAGCCT128640.17723673545474572No Hit
CAACGGAATCCCAAAAGCAGCTGA124560.17161542108397954No Hit
CTTCTCACTACTGCACTTGACTAG123400.17001720425307543No Hit
TTATAAAGCAATGAGACTGATT122170.16832254330306504No Hit
GGAATACCGGGTGCTGTAGGCTT120000.16533277561077028No Hit
CCCCCCACTGCTAAATTTGACTGG113910.15694213724852368No Hit
CACTAGATTGTGAGCTCCTGGAA113610.15652880530949675No Hit
ACCGGGTGCTGTAGGCTTA112410.15487547755338907No Hit
TATTGCACTTGTCCCGGCCTGTAG110070.15165148842897902No Hit
ATCCCACCGCTGCCACCA107930.14870305393058697No Hit
ATCCCGGACGAGCCCCCA104340.14375684839356476No Hit
CGCGACCTCAGATCAGACGTGG103570.14269596308339566No Hit
CAAAGAATTCTCCTTTTGGGCTT101430.13974752858500358No Hit
TACGCCTGTCTGAGCGTCGCT99140.13659242811709804No Hit
TGAGAACTGAATTCCATGGGTT99060.13648220626669086No Hit
TGTAAACATCCCCGACTGGAAGC98900.1362617625658765No Hit
CAAAGTGCTGTTCGTGCAGGTAG98240.13535243230001726No Hit
TGTCAGTTTGTCAAATACCCCA96850.1334373276491925No Hit
AGCAGCATTGTACAGGGCTATGT96200.13254177511463416No Hit
TGTCAGTTTGTCAAATACCCCAA94780.13058533726990673No Hit
ACCCCCCACTGCTAAATTTGACTG93100.12827067841135595No Hit
CAAAGTGCTGTTCGTGCAGGT92170.12698934940037246No Hit
TGAGGTAGTAGGTTGTATAGT91700.1263417960292303No Hit
CACCCGTAGAACCGACCTTGCG89570.12340713926213911No Hit
TTCACAGTGGCTAAGTTCCG86890.11971470727349857No Hit
TGATGTAGTAGGTTGTATAGTT85550.11786849127917831No Hit
ACCACAGGGTAGAACCACGGAC84730.11673871731250471No Hit
TATTGCACTTGTCCCGGCCTGTAAT84380.1162564967169733No Hit
TCGTTTCCCGGGCGGCGCACCA82320.1134182840689884No Hit
ACTGGACTTGGAGTCAGAAGGCT78960.10878896635188684No Hit
ATGCACCTGGGCAAGGATTCTGA77540.1068325285071594No Hit
CCCATAAAGTAGAAAGCACTA77130.10626764152382259No Hit
GGGAATACCGGGTGCTGTAGGCTTT74980.10330542929412963No Hit
AGAGGTAGTAGGTTGCATAGTT73700.10154187968761474No Hit
ATCACATTGCCAGGGATTTCC73600.10140410237460577No Hit
ACGCCTGTCTGAGCGTCGCT73480.101238769598995No Hit
TAGCAGCACGTAAATATTGGCGT73450.1011974364050923No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)