FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947216

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947216
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7795268
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG7008028.990095016617774No Hit
TTCAAGTAATCCAGGATAGGCT3588274.603138724672455No Hit
AAGCTGCCAGTTGAAGAACTGT3290284.220868352441507No Hit
CCCCCCACTGCTAAATTTGACTG3080513.9517692015207175No Hit
TAGCAGCACGTAAATATTGGCG2856613.66454366931323No Hit
AACATTCAACGCTGTCGGTGAGT2839143.64213263738976No Hit
AACATTCAACGCTGTCGGTGAG1752122.24767127955062No Hit
TAGCTTATCAGACTGATGTTGAC1726892.2153054904590834No Hit
TAGCTTATCAGACTGATGTTGACT1356461.7401069469324209No Hit
TAGCTTATCAGACTGATGTTGA1243901.5957116548141768No Hit
TGAGGTAGTAGGTTGTATAGTT1183611.518369862331866No Hit
CCCCCACTGCTAAATTTGACTG1008641.29391317912354No Hit
TATTGCACTTGTCCCGGCCTGTAA974771.2504637428758063No Hit
TATTGCACTTGTCCCGGCCTGT858841.1017453152348322No Hit
TATTGCACTTGTCCCGGCCTGTAT826731.0605536589633608No Hit
TGTAAACATCCTTGACTGGAAGCT739010.9480238524191855No Hit
CATAAAGTAGAAAGCACTACT703680.9027014850547793No Hit
TCGTACGACTCTTAGCGG700390.8984809758946068No Hit
TTCAAGTAATTCAGGATAGGTT672160.8622666982071687No Hit
TGAGGTAGTAGATTGTATAGTT630580.8089266462679666No Hit
AGCAGCATTGTACAGGGCTATGA531760.6821574319189538No Hit
CAACGGAATCCCAAAAGCAGCTGT496340.6367196098966706No Hit
TATTGCACTTGTCCCGGCCTGTA454610.5831871335276735No Hit
ACTGGACTTGGAGTCAGAAGGC415970.5336186004124553No Hit
CCCATAAAGTAGAAAGCACTAC405890.5206876787302245No Hit
TGTAAACATCCCCGACTGGAAGCT391170.5018044280196653No Hit
CGCCTGTCTGAGCGTCGCT359060.4606127717481939No Hit
CAACGGAATCCCAAAAGCAGCT313670.40238513929219627No Hit
CATTGCACTTGTCTCGGTCTGA306730.39348230234034287No Hit
GCCTGTCTGAGCGTCGCT252800.32429930568134413No Hit
TTCTCACTACTGCACTTGACTAG237870.3051466607690717No Hit
TGAGGTAGTAGTTTGTACAGTT231170.2965517029048905No Hit
TAGCTTATCAGACTGATGTTGACA228850.29357553839072625No Hit
TGTAAACATCCTACACTCTCAGCT226430.2904710909233653No Hit
CAACACCAGTCGATGGGCTGT222700.28568613676912713No Hit
CCCCCACTGCTAAATTTGACTGGCTT215730.27674481493131475No Hit
CCCATAAAGTAGAAAGCACT211310.27107470840001907No Hit
CTCCCACTGCTTCACTTGACTAG201410.2583746960335424No Hit
CACCCGGCTGTGTGCACATGTGC200650.2573997455892472No Hit
AACATTCAACGCTGTCGGTGAGTT199540.2559758048087635No Hit
AGCTCGGTCTGAGGCCCCTCAGT199440.2558475218555667No Hit
TTCAAGTAATCCAGGATAGGCTT194270.24921529317529556No Hit
TTAATGCTAATCGTGATAGGGGTT192180.24653417945348383No Hit
TAGCAGCACGTAAATATTGGC184580.23678467501053202No Hit
AACGGAATCCCAAAAGCAGCTG179410.23015244633026086No Hit
TCGTTTCCCGGCCAATGCACCA179080.22972911258471165No Hit
TGAGGTAGTAGTTTGTGCTGTT167610.21501505785304623No Hit
TTCAAGTAATCCAGGATAGGC163670.2099607094970949No Hit
CTTCTCACTACTGCACTTGACTAG158990.20395706728748775No Hit
TGAGAACTGAATTCCATGGGTT153980.19753009133233135No Hit
TTCACAGTGGCTAAGTTCTGC153600.19704261611018375No Hit
CAAAGAATTCTCCTTTTGGGCT152480.19560584703438036No Hit
CCCCCCACTGCTAAATTTGACTGGCT149330.1915649340086832No Hit
TACGCCTGTCTGAGCGTCGCT143810.1844837149922235No Hit
AATGCACCCGGGCAAGGATTCT142010.18217462183468228No Hit
CTACGCCTGTCTGAGCGTCGCT140840.1806737112822805No Hit
TTCACAGTGGCTAAGTTCCGC140500.18023754924141158No Hit
TTATAAAGCAATGAGACTGATT133600.17138602547083692No Hit
CACTAGATTGTGAGCTCCTGGA133370.17109097467848444No Hit
TGAGGTAGTAGGTTGTGTGGTT132280.16969269048864002No Hit
TATTGCACTTGTCCCGGCCTGTT129650.1663188488195659No Hit
ACCGGGTGCTGTAGGCTT125100.16048197444911452No Hit
CGCGACCTCAGATCAGACGTGG123820.1588399526481963No Hit
CCCCCCACTGCTAAATTTGACTGG122330.15692853664556497No Hit
TGTCAGTTTGTCAAATACCCCAA122130.15667197073917152No Hit
TGATGTAGTAGGTTGTATAGTT121590.15597924279190914No Hit
CAAAGAATTCTCCTTTTGGGCTT120510.1545937868973844No Hit
CTCCCACTGCTTCACTTGACTAGCCT120040.15399085701735976No Hit
CAACGGAATCCCAAAAGCAGCTGA112790.14469034291059651No Hit
AGCAGCATTGTACAGGGCTATGT112250.14399761496333416No Hit
CAAAGTGCTGTTCGTGCAGGT109950.14104710703980927No Hit
TTCACAGTGGCTAAGTTCCG108450.13912286274185826No Hit
CACCCGTAGAACCGACCTTGCG108100.1386738724056697No Hit
CACTAGATTGTGAGCTCCTGGAA107340.1376989219613745No Hit
ACCACAGGGTAGAACCACGGAC105380.1351845760787185No Hit
TGAGGTAGTAGGTTGTATAGT104420.13395305972802987No Hit
ACCGGGTGCTGTAGGCTTT102370.13132325918749682No Hit
CAAAGTGCTGTTCGTGCAGGTAG102350.13129760259685747No Hit
TATTGCACTTGTCCCGGCCTGTAG100010.1282957814920539No Hit
TGTAAACATCCCCGACTGGAAGC99900.1281546702435375No Hit
TAGCAGCACGTAAATATTGGCGT98590.12647416355666027No Hit
AACCGGGCGGAAACACCA97760.12540941504512737No Hit
ACGCCTGTCTGAGCGTCGCT96700.12404961574124199No Hit
TGTCAGTTTGTCAAATACCCCA94140.1207655721394056No Hit
ACCGGGTGCTGTAGGCTTA90530.11613455752900348No Hit
CGCGACCTCAGATCAGACGTGGCG90260.11578819355537232No Hit
AGAGGTAGTAGGTTGCATAGTT90230.1157497086694133No Hit
ATCACATTGCCAGGGATTTCC89870.11528789003790504No Hit
ACCCCCCACTGCTAAATTTGACTG87380.11209364450330636No Hit
ATCCCACCGCTGCCACCA87120.11176010882499486No Hit
ATGCACCTGGGCAAGGATTCTGA84620.10855303499507649No Hit
TCCTGTCTGAGCGTCGCT84470.1083606105652814No Hit
TTCAAGTAATCCAGGATAGGCTA83480.10709060932863373No Hit
AATGACACGATCACTCCCGTTG81020.10393484867999407No Hit
AGCAGCATTGTACAGGGCTATG79390.10184383654288728No Hit
TCGTTTCCCGGGCGGCGCACCA78960.10129221984414133No Hit
TTCACCACCTTCTCCACCCAGC78580.10080474462199375No Hit
ATCCCGGACGAGCCCCCA78490.1006892899641167No Hit
ACTGGACTTGGAGTCAGAAGGCT78250.10038141087644453No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)