FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947217

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947217
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11947305
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG6623885.544246170998397No Hit
GCCTGTCTGAGCGTCGCT6583855.510740706795382No Hit
AAGCTGCCAGTTGAAGAACTGT5179784.335521692967577No Hit
TTCAAGTAATCCAGGATAGGCT4609593.8582676176761197No Hit
CCCCCCACTGCTAAATTTGACTG4508563.773704613718324No Hit
AACATTCAACGCTGTCGGTGAGT4499293.765945541693294No Hit
TAGCAGCACGTAAATATTGGCG3571242.9891594798994414No Hit
TAGCTTATCAGACTGATGTTGAC2699822.2597732291926924No Hit
TAGCTTATCAGACTGATGTTGACT2199431.8409423715222806No Hit
AACATTCAACGCTGTCGGTGAG2092531.751466125624147No Hit
TAGCTTATCAGACTGATGTTGA1923451.609944669530074No Hit
TATTGCACTTGTCCCGGCCTGTAA1714421.4349847099408612No Hit
TGAGGTAGTAGGTTGTATAGTT1685681.4109290756367232No Hit
TATTGCACTTGTCCCGGCCTGTAT1570991.3149325308092494No Hit
TATTGCACTTGTCCCGGCCTGT1416531.1856481440793551No Hit
CCCCCACTGCTAAATTTGACTG1398151.1702639214450454No Hit
TGTAAACATCCTTGACTGGAAGCT1320581.1053371450716292No Hit
TCGTACGACTCTTAGCGG1206201.0096000729871717No Hit
TGAGGTAGTAGATTGTATAGTT1121800.9389565261789166No Hit
CGCCTGTCTGAGCGTCGCT1011490.846626080107606No Hit
TTCAAGTAATTCAGGATAGGTT956530.8006240737973961No Hit
AGCAGCATTGTACAGGGCTATGA924500.7738146803818936No Hit
CATAAAGTAGAAAGCACTACT917660.7680895398585706No Hit
TATTGCACTTGTCCCGGCCTGTA740690.6199640839503134No Hit
ACTGGACTTGGAGTCAGAAGGC665470.5570042783707289No Hit
TGTAAACATCCCCGACTGGAAGCT659110.5516809020946565No Hit
TCCTGTCTGAGCGTCGCT595010.49802863490971394No Hit
AACCGGGCGGAAACACCA520090.43531993198466096No Hit
TGTAAACATCCTACACTCTCAGCT497990.41682203643415816No Hit
CCCATAAAGTAGAAAGCACTAC494290.41372510369493376No Hit
CAACGGAATCCCAAAAGCAGCTGT480670.402325043179194No Hit
TTAATGCTAATCGTGATAGGGGTT451030.3775161009114608No Hit
CATTGCACTTGTCTCGGTCTGA442310.37021738375307234No Hit
TGAGGTAGTAGTTTGTACAGTT438370.36691956889022254No Hit
TACGCCTGTCTGAGCGTCGCT372230.31155980365446434No Hit
AACATTCAACGCTGTCGGTGAGTT362660.30354962897490273No Hit
CTACGCCTGTCTGAGCGTCGCT339790.28440723661110184No Hit
AGCTCGGTCTGAGGCCCCTCAGT314700.26340668460376626No Hit
TTCAAGTAATCCAGGATAGGCTT311810.26098772903177747No Hit
TTCTCACTACTGCACTTGACTAG310820.2601590902718228No Hit
TAGCTTATCAGACTGATGTTGACA309890.2593806720427745No Hit
CCCATAAAGTAGAAAGCACT308470.25819211947799103No Hit
ACCGGGTGCTGTAGGCTT294690.2466581375465011No Hit
TGAGGTAGTAGTTTGTGCTGTT289420.24224710091522733No Hit
CAACACCAGTCGATGGGCTGT288540.241510533128601No Hit
CACCCGGCTGTGTGCACATGTGC273860.22922324323351584No Hit
GCGTTCGCGCTTTCCCCTG260760.21825842731896442No Hit
CCCCCACTGCTAAATTTGACTGGCTT253060.2118134591859838No Hit
ACGCCTGTCTGAGCGTCGCT251940.21087600927573202No Hit
CTCCCACTGCTTCACTTGACTAG248890.20832313228799298No Hit
ACCGGGTGCTGTAGGCTTT237010.1983794671685372No Hit
ACCGGGTGCTGTAGGCTTA229020.19169176646950922No Hit
TTATAAAGCAATGAGACTGATT227730.19061202505502287No Hit
CTTCTCACTACTGCACTTGACTAG223990.18748161196186086No Hit
TTCAAGTAATCCAGGATAGGC211790.17727010401090454No Hit
TATTGCACTTGTCCCGGCCTGTT210990.17660049693215332No Hit
ATCCCGGACGAGCCCCCA207550.17372118649352303No Hit
CAAAGAATTCTCCTTTTGGGCT206100.17250752366328642No Hit
AGCAGCATTGTACAGGGCTATGT199510.16699163535207312No Hit
TGTAAACATCCTACACTCAGCT191770.16051318686515495No Hit
CAAAGAATTCTCCTTTTGGGCTT191430.1602286038566857No Hit
CACTAGATTGTGAGCTCCTGGA190650.1595757369549032No Hit
TATTGCACTTGTCCCGGCCTGTAG189470.15858806651374513No Hit
CAACGGAATCCCAAAAGCAGCT188260.15757528580713392No Hit
CCCCCCACTGCTAAATTTGACTGGCT188100.15744136439138368No Hit
TGAGGTAGTAGGTTGTGTGGTT186580.1561691109417563No Hit
TTCACAGTGGCTAAGTTCCG184200.1541770298824714No Hit
ACCACAGGGTAGAACCACGGAC183680.1537417852812831No Hit
GGAATACCGGGTGCTGTAGGCTTT183470.1535660134231109No Hit
TGTCAGTTTGTCAAATACCCCAA181930.15227701979651478No Hit
TCGTTTCCCGGCCAATGCACCA181070.1515571921868572No Hit
AACGGAATCCCAAAAGCAGCTG180740.1512809792668723No Hit
TGAGAACTGAATTCCATGGGTT180550.1511219475856689No Hit
CAAAGTGCTGTTCGTGCAGGT173130.1449113419302512No Hit
TAGCAGCACGTAAATATTGGC169120.14155493644801065No Hit
CAAAGTGCTGTTCGTGCAGGTAG167620.1402994231753521No Hit
TGTAAACATCCCCGACTGGAAGC166450.13932012282267842No Hit
CACTAGATTGTGAGCTCCTGGAA165280.13834082247000473No Hit
TGAGGTAGTAGGTTGTATAGT160380.13423947911265344No Hit
CGCGACCTCAGATCAGACGTGG158830.13294211539757292No Hit
GGGGCTACGCCTGTCTGAGCGTCGCT155930.1305147897370997No Hit
TAGCAGCACGTAAATATTGGCGT154020.12891610283658114No Hit
TGTCAGTTTGTCAAATACCCCA152820.12791169221845428No Hit
TTCACAGTGGCTAAGTTCTGC151990.12721697487424988No Hit
TTCACAGTGGCTAAGTTCCGC151470.12678173027306158No Hit
TCGTTTCCCGGGCGGCGCACCA145050.12140813346608294No Hit
CCCCCCACTGCTAAATTTGACTGG142030.11888036674379704No Hit
AGCAGCATTGTACAGGGCTATG139470.11673762409179309No Hit
ATCCCACCGCTGCCACCA138880.11624378887121405No Hit
CCTCGACACAAGGGTTTGT138000.1155072210845877No Hit
GGAATACCGGGTGCTGTAGGCTT135390.1133226279901618No Hit
AACATTCAACGCTGTCGGTGA134730.11277020215019203No Hit
AGAGGTAGTAGGTTGCATAGTT132690.11106270409937638No Hit
GGGCTACGCCTGTCTGAGCGTCGCT132070.11054375861334419No Hit
TGATGTAGTAGGTTGTATAGTT131810.11032613631275003No Hit
CTCCCACTGCTTCACTTGACTAGCCT131650.11019221489699978No Hit
TATTGCACTTGTCCCGGCCTGTAAT129150.10809969277590219No Hit
CGCGACCTCAGATCAGACGTGGCG124680.10435826322337967No Hit
GCCTGTCTGAGCGTCGCTT121800.10194767773987522No Hit
AACATTCAACGCTGTCGGTGAGA121670.10183886658957815No Hit
GAATACCGGGTGCTGTAGGCTTT120560.10090978676781082No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)