FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947218

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947218
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9053952
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG7713848.51985961489524No Hit
GCCTGTCTGAGCGTCGCT7089817.830624681906863No Hit
CCCCCCACTGCTAAATTTGACTG4562625.039368443746996No Hit
TTCAAGTAATCCAGGATAGGCT4200194.639068110809512No Hit
AAGCTGCCAGTTGAAGAACTGT2929063.2351176591172566No Hit
TAGCAGCACGTAAATATTGGCG2791703.0834049042893095No Hit
AACATTCAACGCTGTCGGTGAGT2063422.2790268824045015No Hit
AACATTCAACGCTGTCGGTGAG1719871.8995793218254304No Hit
TATTGCACTTGTCCCGGCCTGTAA1449301.6007374459241668No Hit
CCCCCACTGCTAAATTTGACTG1407921.555033647185229No Hit
TAGCTTATCAGACTGATGTTGACT1334291.4737100439675404No Hit
TATTGCACTTGTCCCGGCCTGTAT1310171.4470697436876183No Hit
TGAGGTAGTAGGTTGTATAGTT1270021.4027244677241497No Hit
TAGCTTATCAGACTGATGTTGAC1229991.3585117305680436No Hit
TAGCTTATCAGACTGATGTTGA1108681.2245260412248706No Hit
CATAAAGTAGAAAGCACTACT868690.9594594713998925No Hit
TATTGCACTTGTCCCGGCCTGT868210.9589293161704414No Hit
TGTAAACATCCTTGACTGGAAGCT800210.8838239919981904No Hit
TGAGGTAGTAGATTGTATAGTT759150.8384736300788872No Hit
TTCAAGTAATTCAGGATAGGTT619970.684750703339271No Hit
TATTGCACTTGTCCCGGCCTGTA599200.6618104447648938No Hit
CAACGGAATCCCAAAAGCAGCTGT530190.5855895856306726No Hit
CCCATAAAGTAGAAAGCACTAC522850.577482628580315No Hit
TCCTGTCTGAGCGTCGCT475740.5254501017898041No Hit
GGAATACCGGGTGCTGTAGGCTTT453280.5006432550117341No Hit
AACCGGGCGGAAACACCA449960.49697634800803003No Hit
TGTAAACATCCCCGACTGGAAGCT415440.45884935108999914No Hit
AGCAGCATTGTACAGGGCTATGA415290.45868367758079565No Hit
TCGTACGACTCTTAGCGG391990.43294905915118614No Hit
TTAATGCTAATCGTGATAGGGGTT380740.420523545960924No Hit
TGAGAACTGAATTCCATGGGTT361950.3997701777080329No Hit
CCCCCACTGCTAAATTTGACTGGCTT348500.3849147863827862No Hit
GGAATACCGGGTGCTGTAGGCTT322690.35640789789917154No Hit
TTCTCACTACTGCACTTGACTAG321180.35474011790652304No Hit
ACTGGACTTGGAGTCAGAAGGC305970.3379408240732887No Hit
TGAGGTAGTAGTTTGTACAGTT303740.33547781123646336No Hit
CATTGCACTTGTCTCGGTCTGA288820.31899881952102244No Hit
TGTAAACATCCTACACTCTCAGCT236720.2614548873243419No Hit
CAAAGAATTCTCCTTTTGGGCT227010.250730288828569No Hit
CGCCTGTCTGAGCGTCGCT223700.24707442672547855No Hit
CCCATAAAGTAGAAAGCACT222690.24595889176350832No Hit
CAACACCAGTCGATGGGCTGT221070.2441696178641106No Hit
TGATGTAGTAGGTTGTATAGTT212580.2347924972431928No Hit
AGCTCGGTCTGAGGCCCCTCAGT210490.23248411301495744No Hit
CACCCGGCTGTGTGCACATGTGC209470.23135753315237367No Hit
ATCCCGGACGAGCCCCCA208070.22981124706647438No Hit
CTCCCACTGCTTCACTTGACTAG197800.2184681341363418No Hit
TAGCAGCACGTAAATATTGGC197230.2178385748013685No Hit
CTTCTCACTACTGCACTTGACTAG196690.21724215016823592No Hit
ACCGGGTGCTGTAGGCTT192000.21206209178047333No Hit
TTCAAGTAATCCAGGATAGGC190790.2107256588062318No Hit
TTCACAGTGGCTAAGTTCCG189550.20935609113014955No Hit
ACCGGGTGCTGTAGGCTTA185390.20476141247490598No Hit
TAGCTTATCAGACTGATGTTGACA178760.1974386433681115No Hit
AACGGAATCCCAAAAGCAGCTG172380.1903919967766562No Hit
CAACGGAATCCCAAAAGCAGCT169470.1871779306981084No Hit
TATTGCACTTGTCCCGGCCTGTAG168950.18660359586620295No Hit
TGAGGTAGTAGTTTGTGCTGTT164750.18196473760850512No Hit
ACCGGGTGCTGTAGGCTTT161010.1778339447790313No Hit
TTCAAGTAATCCAGGATAGGCTT158320.17486286651398197No Hit
TATTGCACTTGTCCCGGCCTGTT155390.171626710634207No Hit
TTCACAGTGGCTAAGTTCCGC154770.1709419267961659No Hit
CACTAGATTGTGAGCTCCTGGA149170.16475678245256878No Hit
CCCCCCACTGCTAAATTTGACTGGCT148430.16393945980716487No Hit
AACATTCAACGCTGTCGGTGAGTT146900.16224959001328923No Hit
TTCACAGTGGCTAAGTTCTGC146190.161465402069726No Hit
CACTAGATTGTGAGCTCCTGGAA143910.1589471647298329No Hit
ACCCCCCACTGCTAAATTTGACTG141850.15667191520343823No Hit
CAAAGAATTCTCCTTTTGGGCTT134930.14902884397885033No Hit
CCCCCCACTGCTAAATTTGACTGG130210.14381565088924703No Hit
TGATGTAGTAGATTGTATAGTT127790.141142784940764No Hit
CTACGCCTGTCTGAGCGTCGCT124340.1373322942290836No Hit
TCGTTTCCCGGGCGGCGCACCA123100.1359627265530014No Hit
TTATAAAGCAATGAGACTGATT121510.13420658735544433No Hit
TCGTTTCCCGGCCAATGCACCA116520.12869518194927473No Hit
TGTAAACATCCCCGACTGGAAGC116320.12847428393700341No Hit
CCCATAAAGTAGAAAGCACTA116190.12833070022902707No Hit
AGCTACATTGTCTGCTGGGTTT111630.12329422554924081No Hit
TGAGGTAGTAGGTTGTATAGT109590.12104106582407329No Hit
TATTGCACTTGTCCCGGCCTGTAAT108810.1201795635762151No Hit
AGCTACATCTGGCTACTGGGTCTC107990.11927388172590268No Hit
ATGCACCTGGGCAAGGATTCTGA106700.11784908954675262No Hit
TGTCAGTTTGTCAAATACCCCA106640.11778282014307123No Hit
TGTCAGTTTGTCAAATACCCCAA106430.11755087723018633No Hit
ATCACATTGCCAGGGATTTCC106430.11755087723018633No Hit
TGAGGTAGTAGGTTGTGTGGTT103930.11478965207679474No Hit
CTCCCACTGCTTCACTTGACTAGCCT103380.1141821825430486No Hit
ACTGGACTTGGAGTCAGAAGGCT101650.11227141473690162No Hit
TATTGCACTTGTCCCGGCCTGTAAA101520.11212783102892528No Hit
TTCACAGTGGCTAAGTTCTG100490.11099020626572793No Hit
TATTGCACTTGTCCCGGCCTGTTT100330.11081348785591087No Hit
ACTGGACTTGGAGTCAGAAGGCA97420.10759942177736308No Hit
TGAGAACTGAATTCCATAGGCTGT94950.10487133132581221No Hit
GCCTGTCTGAGCGTCGCG94510.10438535569881528No Hit
ATCCCACCGCTGCCACCA93310.10305996762518732No Hit
TGAGGTAGTAAGTTGTATTGTT91950.1015578611417423No Hit
TAGCAGCACGTAAATATTGGCGT91840.10143636723499307No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)