FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947219

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947219
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5670013
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG5193089.15885025307702No Hit
CCCCCCACTGCTAAATTTGACTG3911436.898449791914057No Hit
TTCAAGTAATCCAGGATAGGCT2006513.5388102284774305No Hit
TAGCAGCACGTAAATATTGGCG1729583.0503986498796385No Hit
GCCTGTCTGAGCGTCGCT1633632.8811750519795987No Hit
AAGCTGCCAGTTGAAGAACTGT1602132.8256196237998044No Hit
AACATTCAACGCTGTCGGTGAGT1487152.6228334926216217No Hit
TAGCTTATCAGACTGATGTTGAC1318512.3254091304552564No Hit
TAGCTTATCAGACTGATGTTGACT1152992.0334874011752704No Hit
CCCCCACTGCTAAATTTGACTG1072061.8907540423628657No Hit
AACATTCAACGCTGTCGGTGAG1016591.7929235788348281No Hit
TATTGCACTTGTCCCGGCCTGTAA975291.7200842396657645No Hit
TAGCTTATCAGACTGATGTTGA860551.5177213879403804No Hit
TATTGCACTTGTCCCGGCCTGTAT794431.401107898694412No Hit
TGAGGTAGTAGGTTGTATAGTT689501.216046594602164No Hit
TGTAAACATCCTTGACTGGAAGCT591281.0428194785444056No Hit
AACCGGGCGGAAACACCA462470.8156418688987134No Hit
TCCTGTCTGAGCGTCGCT434600.7664885424424952No Hit
TATTGCACTTGTCCCGGCCTGT430490.7592398818133221No Hit
TTCAAGTAATTCAGGATAGGTT386630.6818855618144086No Hit
TGAGGTAGTAGATTGTATAGTT380040.6702630135063182No Hit
AGCAGCATTGTACAGGGCTATGA377080.6650425669218043No Hit
CCCCCACTGCTAAATTTGACTGGCTT366420.6462419045600072No Hit
TATTGCACTTGTCCCGGCCTGTA342400.6038786859924307No Hit
TGTAAACATCCCCGACTGGAAGCT337450.595148547278463No Hit
CATAAAGTAGAAAGCACTACT334320.5896282777482168No Hit
CAACGGAATCCCAAAAGCAGCTGT334240.589487184597284No Hit
ACTGGACTTGGAGTCAGAAGGC313560.5530146050811524No Hit
CCCATAAAGTAGAAAGCACTAC250090.44107482645983354No Hit
CAACGGAATCCCAAAAGCAGCT239310.4220625243716372No Hit
TAGCTTATCAGACTGATGTTGACA223180.3936146178148092No Hit
CAAAGAATTCTCCTTTTGGGCT183050.322838765978138No Hit
TCGTACGACTCTTAGCGG182370.3216394741952091No Hit
CCCCCCACTGCTAAATTTGACTGGCT182160.32126910467401043No Hit
CATTGCACTTGTCTCGGTCTGA178390.31462008993630175No Hit
TGTAAACATCCTACACTCTCAGCT177570.31317388513924044No Hit
TTCTCACTACTGCACTTGACTAG172950.3050257556728706No Hit
CTTCTCACTACTGCACTTGACTAG154140.27185122855979343No Hit
CACTAGATTGTGAGCTCCTGGAA149710.26403819532688905No Hit
CACCCGGCTGTGTGCACATGTGC148100.2611986956643662No Hit
CTCCCACTGCTTCACTTGACTAG141590.24971724050720873No Hit
TCGTTTCCCGGCCAATGCACCA139500.24603118193908904No Hit
CACTAGATTGTGAGCTCCTGGA136970.2415691110408389No Hit
AGCTCGGTCTGAGGCCCCTCAGT129500.22839453807248764No Hit
TAGCAGCACGTAAATATTGGC124470.21952330620758717No Hit
AACATTCAACGCTGTCGGTGAGTT124430.21945275963212077No Hit
CGCCTGTCTGAGCGTCGCT123490.21779491510866025No Hit
AACGGAATCCCAAAAGCAGCTG121830.21486723222680443No Hit
TGAGGTAGTAGTTTGTACAGTT116120.20479670857897503No Hit
TTCAAGTAATCCAGGATAGGCTT113870.2008284637089897No Hit
CAAAGAATTCTCCTTTTGGGCTT112360.19816533048513293No Hit
CCCATAAAGTAGAAAGCACT111800.19717767842860326No Hit
CTCCCACTGCTTCACTTGACTAGCCT111640.19689549212673763No Hit
CCCCCCACTGCTAAATTTGACTGG110420.19474382157501224No Hit
ACCCCCCACTGCTAAATTTGACTG105450.18597840957331138No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC105130.18541403696958014No Hit
CAACGGAATCCCAAAAGCAGCTGA103760.18299781675985574No Hit
TATTGCACTTGTCCCGGCCTGTAG102910.18149870203119464No Hit
CAACACCAGTCGATGGGCTGT99360.17523769345855117No Hit
TATTGCACTTGTCCCGGCCTGTAAA98870.17437349790908768No Hit
AATGCACCCGGGCAAGGATTCT97910.17268038009789394No Hit
TGAGGTAGTAGTTTGTGCTGTT94840.16726593043084734No Hit
TATTGCACTTGTCCCGGCCTGTAAT92290.16276858624486398No Hit
ATGCACCTGGGCAAGGATTCTGA89820.15841233520981346No Hit
TTCACAGTGGCTAAGTTCCG88930.15684267390568593No Hit
GTCAGGATGGCCGAGCGGTCTAAGG88780.1565781242476869No Hit
TTCAAGTAATCCAGGATAGGC88390.15589029513688946No Hit
TATTGCACTTGTCCCGGCCTGTT87510.15433827047662854No Hit
TGTAAACATCCCCGACTGGAAGC85780.1512871310877065No Hit
TTCACAGTGGCTAAGTTCTGC81190.14319191155293648No Hit
AACTTAACTTGACCGCTCTGACCA77010.1358197944166971No Hit
CTACGCCTGTCTGAGCGTCGCT76470.13486741564790064No Hit
AGCAGCATTGTACAGGGCTATGT75580.1332977543437731No Hit
TGTCAGTTTGTCAAATACCCCAA75490.1331390245489737No Hit
ATCACATTGCCAGGGATTTCCA73510.12964696906338663No Hit
GGAATACCGGGTGCTGTAGGCTTT72310.12753057179939448No Hit
TTATAAAGCAATGAGACTGATT70760.12479689200007124No Hit
TGTCAGTTTGTCAAATACCCCA70370.1241090628892738No Hit
ATCACATTGCCAGGGATTTCC69690.12290977110634489No Hit
CAAAGTGCTGTTCGTGCAGGTAG66890.11797151082369652No Hit
TCGTTTCCCGGGCGGCGCACCA66260.11686040226010064No Hit
CCCCCACTGCTAAATTTGACTGGCT65540.11559056390170536No Hit
TTCACAGTGGCTAAGTTCCGC64930.11451472862584265No Hit
TGAGGTAGTAGGTTGTGTGGTT64480.11372107965184561No Hit
TACGCCTGTCTGAGCGTCGCT62060.10945301183612807No Hit
CACCCGTAGAACCGACCTTGCG61670.1087651827253306No Hit
TAGCAGCACGTAAATATTGGCGT61230.10798917039520015No Hit
CCCATAAAGTAGAAAGCACTA60720.10708970155800347No Hit
TATTGCACTTGTCCCGGCCTGTTT60270.10629605258400641No Hit
ACCGGGTGCTGTAGGCTT57700.10176343511028986No Hit
ACTGGACTTGGAGTCAGAAGGCT56830.10022904709389555No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)