FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947220

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947220
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10139470
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG103308910.188786987880038No Hit
GCCTGTCTGAGCGTCGCT9502749.372028321006917No Hit
CCCCCCACTGCTAAATTTGACTG5659395.581544203000748No Hit
TTCAAGTAATCCAGGATAGGCT3852483.7994885334243307No Hit
AAGCTGCCAGTTGAAGAACTGT3446663.399250651168158No Hit
AACATTCAACGCTGTCGGTGAGT3257513.212702439082122No Hit
TAGCAGCACGTAAATATTGGCG3219123.1748404995527375No Hit
AACATTCAACGCTGTCGGTGAG2439172.405618834120521No Hit
CCCCCACTGCTAAATTTGACTG2051832.0236067565661715No Hit
TCCTGTCTGAGCGTCGCT1948211.9214120659166605No Hit
TAGCTTATCAGACTGATGTTGAC1338261.3198520238237306No Hit
TATTGCACTTGTCCCGGCCTGTAA1323201.304999176485556No Hit
TATTGCACTTGTCCCGGCCTGTAT1294131.2763290388945379No Hit
TGAGGTAGTAGGTTGTATAGTT1288211.270490469422958No Hit
TAGCTTATCAGACTGATGTTGACT1240061.223002780224213No Hit
TAGCTTATCAGACTGATGTTGA1040801.0264836327737052No Hit
AACCGGGCGGAAACACCA1000420.9866590660064086No Hit
TATTGCACTTGTCCCGGCCTGT914800.9022167825339983No Hit
TGAGGTAGTAGATTGTATAGTT765990.7554536874215318No Hit
TTCAAGTAATTCAGGATAGGTT749660.7393483091325286No Hit
CAACGGAATCCCAAAAGCAGCT726870.7168717891566325No Hit
TGTAAACATCCTTGACTGGAAGCT722490.7125520367435378No Hit
CATAAAGTAGAAAGCACTACT709780.7000168647868182No Hit
CAACGGAATCCCAAAAGCAGCTGT660530.6514443062605836No Hit
AGCAGCATTGTACAGGGCTATGA619220.6107025317891369No Hit
TATTGCACTTGTCCCGGCCTGTA573410.565522655523415No Hit
CTCCCACTGCTTCACTTGACTAG519950.512798006207425No Hit
CCCATAAAGTAGAAAGCACTAC462770.45640452607483434No Hit
TGTAAACATCCCCGACTGGAAGCT404200.3986401656102341No Hit
CCCATAAAGTAGAAAGCACT377470.37227784095223915No Hit
ACTGGACTTGGAGTCAGAAGGC373500.3683624489248452No Hit
CATTGCACTTGTCTCGGTCTGA372760.3676326277408977No Hit
TCGTACGACTCTTAGCGG370670.3655713760186676No Hit
TTCTCACTACTGCACTTGACTAG348040.34325265521767906No Hit
TGAGGTAGTAGTTTGTACAGTT335160.33054982163762014No Hit
CGCCTGTCTGAGCGTCGCT310380.30611067442381107No Hit
CCCTGTCTGAGCGTCGCT298890.29477872117576165No Hit
CACCCGGCTGTGTGCACATGTGC278300.27447193985484447No Hit
TGTAAACATCCTACACTCTCAGCT268800.2651026138447079No Hit
AACGGAATCCCAAAAGCAGCTG265330.2616803442389No Hit
CAACACCAGTCGATGGGCTGT253050.2495692575647445No Hit
CCCCCACTGCTAAATTTGACTGGCTT248940.24551579125930645No Hit
CTTCTCACTACTGCACTTGACTAG228260.2251202479025038No Hit
TAGCAGCACGTAAATATTGGC227680.22454822589346385No Hit
TTCAAGTAATCCAGGATAGGCTT205260.20243661650954145No Hit
CCCCCCACTGCTAAATTTGACTGGCT203490.2006909631371265No Hit
CTCCCACTGCTTCACTTGACTAGCCT202850.20005976643749623No Hit
TCGTTTCCCGGCCAATGCACCA198960.19622327399755607No Hit
AACATTCAACGCTGTCGGTGAGTT191740.18910258622985224No Hit
CAACGGAATCCCAAAAGCAGCTGA191390.18875740053474194No Hit
AGCTCGGTCTGAGGCCCCTCAGT186770.18420094935928602No Hit
TGATGTAGTAGGTTGTATAGTT181420.17892453944831435No Hit
TGAGGTAGTAGTTTGTGCTGTT180930.17844127947515995No Hit
TAGCTTATCAGACTGATGTTGACA174910.17250408551926286No Hit
CCCCCCACTGCTAAATTTGACTGG172610.17023572237996662No Hit
TTCAAGTAATCCAGGATAGGC171610.16924947753679434No Hit
CTACGCCTGTCTGAGCGTCGCT163130.16088612126669344No Hit
TATTGCACTTGTCCCGGCCTGTT161210.15899253116780265No Hit
TTCACAGTGGCTAAGTTCTGC160400.1581936728448331No Hit
TGAGGTAGTAGGTTGTGTGGTT152910.15080669896947277No Hit
TTCACAGTGGCTAAGTTCCGC146760.14474129318396328No Hit
CAAAGAATTCTCCTTTTGGGCT146630.14461308135435086No Hit
AGCAGCATTGTACAGGGCTATGT141190.13924790940749368No Hit
TATTGCACTTGTCCCGGCCTGTAG140590.1386561625015903No Hit
CACTAGATTGTGAGCTCCTGGA140000.13807427804411868No Hit
AATGCACCCGGGCAAGGATTCT137950.13605247611561552No Hit
GGAATACCGGGTGCTGTAGGCTTT135580.13371507583729722No Hit
ACCCCCCACTGCTAAATTTGACTG124920.12320170580908076No Hit
CAAAGTGCTGTTCGTGCAGGT124080.12237326014081604No Hit
TGTCAGTTTGTCAAATACCCCAA123210.12151522712725617No Hit
TCCCACTGCTTCACTTGACTAG120170.11851704280401243No Hit
CACTAGATTGTGAGCTCCTGGAA119910.11826061914478765No Hit
GCCTGTCTGAGCGTCGCTC118620.11698836329709542No Hit
TTCACAGTGGCTAAGTTCCG117590.11597253110862797No Hit
TGTAAACATCCCCGACTGGAAGC116830.11522298502781704No Hit
ATCACATTGCCAGGGATTTCC116250.11465096301877711No Hit
TTATAAAGCAATGAGACTGATT116140.11454247608602816No Hit
ACCACAGGGTAGAACCACGGAC116110.114512888740733No Hit
CAAAGTGCTGTTCGTGCAGGTAG114310.11273764802302291No Hit
TGAGGTAGTAGGTTGTATAGT114220.11264888598713739No Hit
AGAGGTAGTAGGTTGCATAGTT113160.11160346645337478No Hit
CAAAGAATTCTCCTTTTGGGCTT113120.11156401665964791No Hit
CCCATAAAGTAGAAAGCACTA109430.10792477318834219No Hit
AATGACACGATCACTCCCGTTG109260.10775711156500291No Hit
TGATGTAGTAGATTGTATAGTT107730.10624815695494932No Hit
TATTGCACTTGTCCCGGCCTGTAAT105980.10452222847939782No Hit
TACGCCTGTCTGAGCGTCGCT105660.10420663012958271No Hit
TGTCAGTTTGTCAAATACCCCA104850.10340777180661317No Hit
AACATTCAACGCTGTCGGTGA103600.10217496575264781No Hit
TATTGCACTTGTCCCGGCCTGTTT101490.10009398913355431No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)