FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947221

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947221
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6349325
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG67406810.616372606536915No Hit
CCCCCCACTGCTAAATTTGACTG5025337.914746843168369No Hit
TTCAAGTAATCCAGGATAGGCT2113103.3280703066861435No Hit
TAGCAGCACGTAAATATTGGCG2040933.2144046808125273No Hit
AAGCTGCCAGTTGAAGAACTGT2015753.174746921916897No Hit
AACATTCAACGCTGTCGGTGAGT1952893.0757442720289165No Hit
GCCTGTCTGAGCGTCGCT1431582.2546963653616725No Hit
CCCCCACTGCTAAATTTGACTG1396602.1996038949022143No Hit
AACATTCAACGCTGTCGGTGAG1292112.035035220279321No Hit
TAGCTTATCAGACTGATGTTGAC1264921.9922117705425382No Hit
TAGCTTATCAGACTGATGTTGACT1163591.8326200029136954No Hit
TATTGCACTTGTCCCGGCCTGTAA1057291.6652006315631975No Hit
TATTGCACTTGTCCCGGCCTGTAT947061.4915916258814914No Hit
TGTAAACATCCTTGACTGGAAGCT721861.136908254027003No Hit
TGAGGTAGTAGGTTGTATAGTT659691.0389923338307616No Hit
TAGCTTATCAGACTGATGTTGA595310.9375957286798202No Hit
CAACGGAATCCCAAAAGCAGCTGT481520.7583798277769684No Hit
AGCAGCATTGTACAGGGCTATGA435480.6858681828383332No Hit
TGTAAACATCCCCGACTGGAAGCT423190.6665117945608392No Hit
TCCTGTCTGAGCGTCGCT402940.6346186405641545No Hit
CCCCCACTGCTAAATTTGACTGGCTT401880.6329491717623527No Hit
TGAGGTAGTAGATTGTATAGTT391920.617262464907687No Hit
TATTGCACTTGTCCCGGCCTGT361660.5696038555279498No Hit
TATTGCACTTGTCCCGGCCTGTA358060.5639339614840947No Hit
CATAAAGTAGAAAGCACTACT316130.49789544557886073No Hit
CCCATAAAGTAGAAAGCACTAC310480.4889968618711438No Hit
ACTGGACTTGGAGTCAGAAGGC310420.48890236363707956No Hit
AACCGGGCGGAAACACCA309790.48791013217940493No Hit
CAACGGAATCCCAAAAGCAGCT305430.48104326050406937No Hit
TTCAAGTAATTCAGGATAGGTT289930.45663121670413787No Hit
TTCTCACTACTGCACTTGACTAG251050.39539636103050324No Hit
TTAATGCTAATCGTGATAGGGGTT250310.3942308828103775No Hit
CAAAGAATTCTCCTTTTGGGCT235850.371456808400893No Hit
TAGCTTATCAGACTGATGTTGACA226960.3574553200537065No Hit
TGTCAGTTTGTCAAATACCCCAA226290.3564000897733224No Hit
CTTCTCACTACTGCACTTGACTAG219480.34567454020702987No Hit
CCCCCCACTGCTAAATTTGACTGGCT215330.3391384123509192No Hit
TGTAAACATCCTACACTCTCAGCT208810.3288696042492706No Hit
GGAATACCGGGTGCTGTAGGCTTT199760.31461612061124605No Hit
CTCCCACTGCTTCACTTGACTAG175290.27607659081870906No Hit
CTCCCACTGCTTCACTTGACTAGCCT166070.2615553621841692No Hit
CAAAGAATTCTCCTTTTGGGCTT164670.2593504033893367No Hit
TGAGGTAGTAGTTTGTACAGTT160360.25256228024238797No Hit
TAGCAGCACGTAAATATTGGC156750.24687663649285554No Hit
CCCCCCACTGCTAAATTTGACTGG154060.24263996566564164No Hit
AACATTCAACGCTGTCGGTGAGTT151970.23934827717907023No Hit
CCCATAAAGTAGAAAGCACT149190.2349698590007599No Hit
AACGGAATCCCAAAAGCAGCTG146520.23076468758490074No Hit
CATTGCACTTGTCTCGGTCTGA132110.20806936170380316No Hit
TGTAAACATCCCCGACTGGAAGC129030.2032184523551716No Hit
TGTCAGTTTGTCAAATACCCCA127290.20047800356730833No Hit
TTCAAGTAATCCAGGATAGGCTT126170.19871403653144235No Hit
CACCCGGCTGTGTGCACATGTGC121930.19203616132423526No Hit
TATTGCACTTGTCCCGGCCTGTAG119950.18891771960011497No Hit
CACTAGATTGTGAGCTCCTGGAA118560.18672851051095984No Hit
CGCCTGTCTGAGCGTCGCT117740.18543703464541506No Hit
ACCCCCCACTGCTAAATTTGACTG116690.18378331554929067No Hit
TATTGCACTTGTCCCGGCCTGTAAT114370.18012938383213964No Hit
GGAATACCGGGTGCTGTAGGCTT113420.178633161792789No Hit
CTACGCCTGTCTGAGCGTCGCT111930.1762864556468601No Hit
ACTGGACTTGGAGTCAGAAGGCA110570.17414449567473708No Hit
AGCTCGGTCTGAGGCCCCTCAGT105330.1658916498997925No Hit
CAACGGAATCCCAAAAGCAGCTGA103440.1629149555267686No Hit
ATCACATTGCCAGGGATTTCCA102960.1621589696542546No Hit
TCGTACGACTCTTAGCGG102100.16080449496600033No Hit
TGAGGTAGTAGTTTGTGCTGTT99520.15674107090123754No Hit
ATCACATTGCCAGGGATTTCC98270.15477235769156564No Hit
TCGTTTCCCGGCCAATGCACCA98230.15470935886885614No Hit
CACTAGATTGTGAGCTCCTGGA97440.15346513212034352No Hit
ATGCACCTGGGCAAGGATTCTGA97320.153276135652215No Hit
CCCCCACTGCTAAATTTGACTGGCTA97230.1531343883011186No Hit
TATTGCACTTGTCCCGGCCTGTAAA95980.1511656750914467No Hit
AATGCACCCGGGCAAGGATTCT92290.1453540336964953No Hit
ACTGGACTTGGAGTCAGAAGGCT92190.14519653663972154No Hit
TTCACAGTGGCTAAGTTCTGC89590.141101613163604No Hit
GGGAATACCGGGTGCTGTAGGCTTT81380.128171104802479No Hit
TATTGCACTTGTCCCGGCCTGTT80560.12687962893693425No Hit
TTCAAGTAATCCAGGATAGGC80280.12643863717796772No Hit
AGCAGCATTGTACAGGGCTATGT80270.12642288747229036No Hit
TAGCAGCACGTAAATATTGGCGT79040.1244856736739732No Hit
CAACACCAGTCGATGGGCTGT78400.12347769251062121No Hit
TGAGAACTGAATTCCATAGGCTGT77820.12256420958133346No Hit
CTACGGGGATGATTTTACGAACTGAA75640.11913077374366567No Hit
TTCACAGTGGCTAAGTTCCGC75420.11878428021876342No Hit
TACGCCTGTCTGAGCGTCGCT75160.11837478787115165No Hit
TATTGCACTTGTCCCGGCCTGTTT74890.11794954581786253No Hit
CCCCCCACTGCTAAATTTGACTGTA74410.11719355994534851No Hit
CCCCCACTGCTAAATTTGACTGGCT68290.10755474007079494No Hit
TGAGGTAGTAGGTTGTGTGGTT67600.10646801037905605No Hit
AACATTCAACGCTGTCGGTGAGA65590.10330231953790364No Hit
TTCACAGTGGCTAAGTTCCG64050.10087686486358786No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)