FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947222

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947222
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10361347
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG6905656.664818773080372No Hit
AAGCTGCCAGTTGAAGAACTGT4942044.769688728695217No Hit
CCCCCCACTGCTAAATTTGACTG3881853.7464723457287934No Hit
AACATTCAACGCTGTCGGTGAGT3574283.449628701750844No Hit
TTCAAGTAATCCAGGATAGGCT3559003.4348815844117566No Hit
TAGCAGCACGTAAATATTGGCG3122213.013324425868567No Hit
GCCTGTCTGAGCGTCGCT2994412.889981389485363No Hit
AACATTCAACGCTGTCGGTGAG1944971.8771401054322376No Hit
TAGCTTATCAGACTGATGTTGAC1936521.8689847951236456No Hit
TATTGCACTTGTCCCGGCCTGTAT1571291.5164920159512079No Hit
TATTGCACTTGTCCCGGCCTGTAA1551161.4970640400326327No Hit
TAGCTTATCAGACTGATGTTGACT1436151.3860649585425524No Hit
CGCCTGTCTGAGCGTCGCT1398751.3499692655790796No Hit
CCCCCACTGCTAAATTTGACTG1390491.3419973291117457No Hit
TATTGCACTTGTCCCGGCCTGT1305371.2598458482280344No Hit
TAGCTTATCAGACTGATGTTGA1219971.177424132209837No Hit
TGAGGTAGTAGGTTGTATAGTT1161361.1208581278090581No Hit
TGTAAACATCCTTGACTGGAAGCT1095841.057623106339359No Hit
AGCAGCATTGTACAGGGCTATGA981150.9469328650029769No Hit
CATAAAGTAGAAAGCACTACT956340.9229881018365662No Hit
TCCTGTCTGAGCGTCGCT808100.7799179006358922No Hit
TATTGCACTTGTCCCGGCCTGTA767560.7407917136642562No Hit
TGAGGTAGTAGATTGTATAGTT747180.7211224563756045No Hit
TCGTACGACTCTTAGCGG685430.661525957966662No Hit
TTCAAGTAATTCAGGATAGGTT660310.6372820059013563No Hit
TGTAAACATCCCCGACTGGAAGCT591550.5709199778754636No Hit
AACCGGGCGGAAACACCA557810.5383566441699134No Hit
TTAATGCTAATCGTGATAGGGGTT530590.5120859285959635No Hit
CCCATAAAGTAGAAAGCACTAC528390.5099626525392886No Hit
CAACGGAATCCCAAAAGCAGCTGT466790.4505109229523922No Hit
TGTAAACATCCTACACTCTCAGCT465880.44963265876531305No Hit
ACTGGACTTGGAGTCAGAAGGC459430.44340759941733443No Hit
CCCATAAAGTAGAAAGCACT430230.41522593539237707No Hit
CTACGCCTGTCTGAGCGTCGCT403330.38926405997212526No Hit
CATTGCACTTGTCTCGGTCTGA379130.36590802334870165No Hit
ACGCCTGTCTGAGCGTCGCT363170.3505046206830058No Hit
TACGCCTGTCTGAGCGTCGCT355520.3431214107586591No Hit
TGAGGTAGTAGTTTGTACAGTT330190.31867478234248886No Hit
TTCTCACTACTGCACTTGACTAG307850.29711387911243586No Hit
ATCCCGGACGAGCCCCCA292040.281855245268786No Hit
AACATTCAACGCTGTCGGTGAGTT288620.27855451612613685No Hit
CTCCCACTGCTTCACTTGACTAG287410.2773867142949657No Hit
TGATGTAGTAGGTTGTATAGTT286900.2768945003000092No Hit
GCGTTCGCGCTTTCCCCTG275300.26569904472845085No Hit
CAACACCAGTCGATGGGCTGT267540.2582096710012704No Hit
TTCAAGTAATCCAGGATAGGCTT261510.25238996435502065No Hit
AGCTCGGTCTGAGGCCCCTCAGT255430.24652200143475556No Hit
CTTCTCACTACTGCACTTGACTAG243720.2352203820603634No Hit
CAACGGAATCCCAAAAGCAGCT235470.22725809684783263No Hit
CACCCGGCTGTGTGCACATGTGC227770.21982663064947058No Hit
TGTAAACATCCTACACTCAGCT223750.2159468262186374No Hit
TAGCTTATCAGACTGATGTTGACA222650.21488518819029997No Hit
AGCAGCATTGTACAGGGCTATGT220900.21319621859976312No Hit
TATTGCACTTGTCCCGGCCTGTT214170.20670092411729865No Hit
ATCCCACCGCTGCCACCA206470.19926945791893658No Hit
TGAGGTAGTAGTTTGTGCTGTT201090.19407708283488626No Hit
TTATAAAGCAATGAGACTGATT196570.18971471566389966No Hit
TTCACAGTGGCTAAGTTCCG191880.18518827716126096No Hit
TGATGTAGTAGATTGTATAGTT188660.1820805731146732No Hit
GGAATACCGGGTGCTGTAGGCTTT185980.1794940368274511No Hit
TATTGCACTTGTCCCGGCCTGTAG181680.17534399726213204No Hit
TGTCAGTTTGTCAAATACCCCAA177970.17176338173019395No Hit
TGTAAACATCCCCGACTGGAAGC174550.16846265258754484No Hit
TTCAAGTAATCCAGGATAGGC172480.16646484284330987No Hit
TAGCAGCACGTAAATATTGGC171710.16572169622347366No Hit
ACCACAGGGTAGAACCACGGAC171700.1657120449686706No Hit
CAAAGAATTCTCCTTTTGGGCT171010.16504610838725892No Hit
CAAAGAATTCTCCTTTTGGGCTT165690.15991164083202697No Hit
TGTCAGTTTGTCAAATACCCCA158920.1533777413303502No Hit
AACGGAATCCCAAAAGCAGCTG158910.15336809007554714No Hit
AGCAGCATTGTACAGGGCTATG147750.14259728971532368No Hit
TGAGGTAGTAGGTTGTGTGGTT144510.1394702831591298No Hit
CCCCCACTGCTAAATTTGACTGGCTT144170.1391421404958255No Hit
TTCACAGTGGCTAAGTTCCGC141740.13679688557868006No Hit
TAGCAGCACGTAAATATTGGCGT140970.13605373895884387No Hit
CAAAGTGCTGTTCGTGCAGGTAG137740.13293638365745306No Hit
TTCACAGTGGCTAAGTTCTGC137640.13283987110942236No Hit
CCAAGTCCTTCTGATCGAGGCCC137620.13282056859981622No Hit
TCGTTTCCCGGCCAATGCACCA136310.13155625422061437No Hit
GGAATACCGGGTGCTGTAGGCTT135970.13122811155731007No Hit
CCTCGACACAAGGGTTTGT135360.13063938501432293No Hit
ACCGGGTGCTGTAGGCTT133440.12878634409213396No Hit
CACTAGATTGTGAGCTCCTGGA132910.1282748275875714No Hit
CCCCCCACTGCTAAATTTGACTGGCT131300.1267209755642775No Hit
CCCCCCACTGCTAAATTTGACTGG130520.12596817768963822No Hit
CAAAGTGCTGTTCGTGCAGGT128460.1239800192002063No Hit
TACCTGGTTGATCCTGCC126350.12194360443675906No Hit
TATTGCACTTGTCCCGGCCTGTTT125980.12158650800904554No Hit
TGAGGTAGTAGGTTGTATAGT124290.11995544594732711No Hit
CCCTGTCTGAGCGTCGCT120590.11638448167019212No Hit
CACTAGATTGTGAGCTCCTGGAA120070.11588261642043259No Hit
ACTGGACTTGGAGTCAGAAGGCA116780.11270735359022335No Hit
TATTGCACTTGTCCCGGCCTGA114680.11068059008157916No Hit
ATCACATTGCCAGGGATTTCC114470.11047791373071474No Hit
ACTTGACTTGGAGTCAGAAGGC112280.10836428892884295No Hit
ACCGGGTGCTGTAGGCTTT110220.106376130439411No Hit
AGAGGTAGTAGGTTGCATAGTT109780.10595147522807603No Hit
TGTCAGTTTGTCAAATACCCC108940.10514076982461835No Hit
ATCACATTGCCAGGGATTTCCA108780.10498634974776928No Hit
AACATTCAACGCTGTCGGTGA108560.1047740221421018No Hit
AATGACACGATCACTCCCGTTG108140.10436866944037294No Hit
TCGTTTCCCGGGCGGCGCACCA106610.1028920274555036No Hit
TATTGCACTTGTCCCGGCCTGTAAT106300.10259283855660851No Hit
AACATTCAACGCTGTCGGTGAGA105400.10172422562433243No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)