FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947224

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947224
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7739116
Sequences flagged as poor quality0
Sequence length18-26
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG5600557.236679227963504No Hit
TTCAAGTAATCCAGGATAGGCT3483894.501664014339622No Hit
GCCTGTCTGAGCGTCGCT3203884.139852665343174No Hit
TAGCAGCACGTAAATATTGGCG2959893.8245840997860734No Hit
CCCCCCACTGCTAAATTTGACTG2859753.695189476420821No Hit
AAGCTGCCAGTTGAAGAACTGT2849213.681570349895259No Hit
TAGCTTATCAGACTGATGTTGAC2003592.588913255725848No Hit
TGAGGTAGTAGGTTGTATAGTT1919572.4803478846938076No Hit
AACATTCAACGCTGTCGGTGAGT1835892.372221840323882No Hit
TAGCTTATCAGACTGATGTTGA1795252.3197093828287363No Hit
TAGCTTATCAGACTGATGTTGACT1602162.0702106028647203No Hit
TCCTGTCTGAGCGTCGCT1449221.8725911331475067No Hit
AACATTCAACGCTGTCGGTGAG1362851.7609892395979074No Hit
CATAAAGTAGAAAGCACTACT1329441.7178189343589112No Hit
CCCCCACTGCTAAATTTGACTG1285031.6604351194632567No Hit
TGAGGTAGTAGATTGTATAGTT1274211.6464541945100706No Hit
TGTAAACATCCTTGACTGGAAGCT885681.1444201120644788No Hit
TTCAAGTAATTCAGGATAGGTT710010.917430362847643No Hit
TATTGCACTTGTCCCGGCCTGTAA697090.900735949687277No Hit
TATTGCACTTGTCCCGGCCTGTAT633020.817948716623449No Hit
AGCAGCATTGTACAGGGCTATGA522060.6745731683050106No Hit
TGAGGTAGTAGTTTGTACAGTT501740.6483169395574379No Hit
TATTGCACTTGTCCCGGCCTGT500540.6467663748676206No Hit
TGTAAACATCCCCGACTGGAAGCT357590.4620553561931363No Hit
TATTGCACTTGTCCCGGCCTGTA356020.4600267007239586No Hit
CGCCTGTCTGAGCGTCGCT321480.41539628040205107No Hit
CCCATAAAGTAGAAAGCACTAC319390.41269571356728596No Hit
CAACGGAATCCCAAAAGCAGCTGT318330.41132604809128076No Hit
ACTGGACTTGGAGTCAGAAGGC302380.390716459089126No Hit
TTCTCACTACTGCACTTGACTAG295910.3823563311365277No Hit
CATTGCACTTGTCTCGGTCTGA290080.37482317101849877No Hit
CAACACCAGTCGATGGGCTGT282900.36554562562442533No Hit
CAACGGAATCCCAAAAGCAGCT275080.35544111239578263No Hit
TCGTACGACTCTTAGCGG271110.3503113275469705No Hit
TTATAAAGCAATGAGACTGATT262110.33868209237334085No Hit
TTCACAGTGGCTAAGTTCCG259960.3359039973040849No Hit
AACCGGGCGGAAACACCA246270.31821463846775266No Hit
TGAGGTAGTAGTTTGTGCTGTT239210.30909214954266095No Hit
CCCATAAAGTAGAAAGCACT222980.2881207621128821No Hit
TGTAAACATCCTACACTCTCAGCT222220.2871387378093312No Hit
TAGCTTATCAGACTGATGTTGACA220740.28522637469188983No Hit
TAGCAGCACGTAAATATTGGC209790.27107747189730713No Hit
CCCCCACTGCTAAATTTGACTGGCTT206740.2671364533106882No Hit
TTCAAGTAATCCAGGATAGGCTT189780.2452218056946039No Hit
CAAAGAATTCTCCTTTTGGGCT181430.23443245972795862No Hit
CTTCTCACTACTGCACTTGACTAG177570.22944480997571298No Hit
AGAATTACTCTGAGACCT172620.22304873063021669No Hit
TTCACAGTGGCTAAGTTCTGC170510.2203223210506213No Hit
TTCACAGTGGCTAAGTTCCGC168000.21707905657442012No Hit
TGAGGTAGTAGGTTGTATAGT167830.21685939324336267No Hit
AACGGAATCCCAAAAGCAGCTG164100.21203972133251398No Hit
CTCCCACTGCTTCACTTGACTAG157540.20356330102817943No Hit
TGTCAGTTTGTCAAATACCCCAA156860.20268464770394964No Hit
TTCAAGTAATCCAGGATAGGC155080.20038464341405401No Hit
TTCACAGTGGCTAAGTTCTG152180.19663744541366224No Hit
TGTCAGTTTGTCAAATACCCCA152120.19655991717917137No Hit
TGAGGTAGTAAGTTGTATTGTT148980.19250260624081614No Hit
CTACGCCTGTCTGAGCGTCGCT145210.18763124884030682No Hit
AACATTCAACGCTGTCGGTGAGTT143310.18517618808142944No Hit
TGTCAGTTTGTCAAATACC130790.16899862981766908No Hit
TACGCCTGTCTGAGCGTCGCT127440.16466997005859582No Hit
CCCTGTCTGAGCGTCGCT127070.1641918792792355No Hit
CAAAGAATTCTCCTTTTGGGCTT125470.16212445969281244No Hit
TAGCAGCACGTAAATATTGGCGT122780.15864861051313872No Hit
TGTAAACATCCTACACTCAGCT118560.15319579135394792No Hit
AGCAGCATTGTACAGGGCTATGT118350.1529244425332299No Hit
AGAGGTAGTAGGTTGCATAGTT116530.15057275275367368No Hit
TGAGGTAGTAGATTGTATAGT116380.15037893216744652No Hit
TGTAAACATCCCCGACTGGAAGC110090.1422513889183209No Hit
CACCCGGCTGTGTGCACATGTGC108720.14048116089744617No Hit
TCGTTTCCCGGCCAATGCACCA108660.1404036326629553No Hit
CCCCCCACTGCTAAATTTGACTGGCT108590.14031318305604928No Hit
ATCACATTGCCAGGGATTTCC103710.134007553317459No Hit
TAGCAGCACATAATGGTTTGT102520.13246991000005684No Hit
TAGCTTATCAGACTGATGTTG101070.13059631099986097No Hit
AGCTCGGTCTGAGGCCCCTCAGT100120.12936878062042226No Hit
TGAGGTAGTAGGTTGTGTGGTT99870.12904574631004367No Hit
TATTGCACTTGTCCCGGCCTGTT98780.127637316716793No Hit
AATGCACCCGGGCAAGGATTCT96450.12462663694406441No Hit
TGAGGTAGTAGGTTGTATAGTTT93060.12024629169533059No Hit
TGTAAACATCCTTGACTGGAAGC89150.11519403508100926No Hit
AGCAGCATTGTACAGGGCTATG88760.11469010155681864No Hit
TGTCAGTTTGTCAAATACCCC87330.11284234530145304No Hit
CTCCCACTGCTTCACTTGACTAGCCT86830.11219627668069582No Hit
CAACGGAATCCCAAAAGCAGCTGA85900.11099458904608743No Hit
TGAGGTAGTAGGTTGTATAGTTA85850.11092998218401172No Hit
TAGTGGGTTATCAGAACT85340.11027099219083937No Hit
CAAAGTGCTGTTCGTGCAGGT85060.10990919376321533No Hit
TCAGTGCACTACAGAACTTTGT83110.10738952614226223No Hit
CCCCCCACTGCTAAATTTGACTGG83100.10737660476984709No Hit
TCGACCGGACCTCGACCGGCT81720.10559345537655722No Hit
ACGCCTGTCTGAGCGTCGCT80870.10449513872126998No Hit
TGTCAGTTTGTCAAATACCC79470.10268614658314981No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)