FastQCFastQC Report
Fri 9 Sep 2022
EGAF00003947225

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947225
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6421364
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG5133577.994516429842631No Hit
CCCCCCACTGCTAAATTTGACTG4770487.429075816290745No Hit
AACATTCAACGCTGTCGGTGAGT2909674.531233551002559No Hit
AAGCTGCCAGTTGAAGAACTGT1869662.9116243838536486No Hit
AACATTCAACGCTGTCGGTGAG1831682.8524780716371163No Hit
TTCAAGTAATCCAGGATAGGCT1774342.7631824017451745No Hit
TAGCAGCACGTAAATATTGGCG1756342.735150974154401No Hit
CCCCCACTGCTAAATTTGACTG1548392.411310120404325No Hit
TAGCTTATCAGACTGATGTTGACT1163791.812371950881464No Hit
TAGCTTATCAGACTGATGTTGAC1059031.6492290423031617No Hit
TAGCTTATCAGACTGATGTTGA926641.4430578923730224No Hit
TATTGCACTTGTCCCGGCCTGTAA885711.379317540634669No Hit
TATTGCACTTGTCCCGGCCTGTAT785821.223758690521204No Hit
TATTGCACTTGTCCCGGCCTGT620810.9667883645904515No Hit
TGAGGTAGTAGGTTGTATAGTT615760.9589239918497068No Hit
CGCCTGTCTGAGCGTCGCT495590.7717830666506369No Hit
TGTAAACATCCTTGACTGGAAGCT430090.6697798162508775No Hit
AGCAGCATTGTACAGGGCTATGA404240.6295235716274611No Hit
TATTGCACTTGTCCCGGCCTGTA364040.5669200500080668No Hit
CATAAAGTAGAAAGCACTACT362570.5646308167548203No Hit
ACTGGACTTGGAGTCAGAAGGC333450.51928219611908No Hit
TTCAAGTAATTCAGGATAGGTT315630.4915310828042142No Hit
TGTAAACATCCCCGACTGGAAGCT310500.48354212594084367No Hit
TGAGGTAGTAGATTGTATAGTT283110.44088763695688327No Hit
CAACGGAATCCCAAAAGCAGCTGT282070.4392680433627497No Hit
CAACACCAGTCGATGGGCTGT230410.3588178461772296No Hit
CATTGCACTTGTCTCGGTCTGA213370.332281428057964No Hit
CAACGGAATCCCAAAAGCAGCT200040.3115225986254634No Hit
AACATTCAACGCTGTCGGTGAGTT198690.30942024155615533No Hit
TTCTCACTACTGCACTTGACTAG195320.30417213539054944No Hit
CCCATAAAGTAGAAAGCACTAC194900.30351806874676474No Hit
TCGTACGACTCTTAGCGG187180.2914957009133885No Hit
TGATGTAGTAGGTTGTATAGTT177220.27598497764649377No Hit
CACCCGGCTGTGTGCACATGTGC175970.27403835073046784No Hit
CTACGCCTGTCTGAGCGTCGCT169250.2635732844299124No Hit
CCCATAAAGTAGAAAGCACT165260.2573596513139576No Hit
CCCCCACTGCTAAATTTGACTGGCTT161110.25089684995275147No Hit
CTTCTCACTACTGCACTTGACTAG157700.2455864517258327No Hit
CTCCCACTGCTTCACTTGACTAG146740.22851842692611724No Hit
ACCGGGTGCTGTAGGCTT141220.2199221224649467No Hit
TAGCTTATCAGACTGATGTTGACA138050.21498547660590492No Hit
TACGCCTGTCTGAGCGTCGCT134470.20941033711840662No Hit
TGAGGTAGTAGTTTGTACAGTT130720.20357045637032878No Hit
CCCCCCACTGCTAAATTTGACTGG124720.1942266471734043No Hit
CGCGACCTCAGATCAGACGTGG123420.1922021551807373No Hit
CGCGACCTCAGATCAGACGTGGCG122990.19153251552162437No Hit
ACGCCTGTCTGAGCGTCGCT122470.1907227187245576No Hit
CAAAGAATTCTCCTTTTGGGCT116450.18134776349697665No Hit
ACTTGACTTGGAGTCAGAAGGC115220.17943228261160712No Hit
CACTAGATTGTGAGCTCCTGGA114940.1789962381824173No Hit
TGTCAGTTTGTCAAATACCCCAA107780.16784595920742074No Hit
ACCCCCCACTGCTAAATTTGACTG106590.16599277038336402No Hit
CCCCCCACTGCTAAATTTGACTGGCT105960.16501167041768697No Hit
TATTGCACTTGTCCCGGCCTGTT105730.16465349106513819No Hit
TAGCAGCACGTAAATATTGGC103500.1611807086469479No Hit
TTCAAGTAATCCAGGATAGGCTT102720.1599660134513477No Hit
TATTGCACTTGTCCCGGCCTGTAG102070.15895376745501424No Hit
TGTAAACATCCTACACTCTCAGCT100220.15607275961929584No Hit
ACCGGGTGCTGTAGGCTTT97910.1524753930784799No Hit
TGAGGTAGTAGTTTGTGCTGTT97870.15241310101716707No Hit
CAAAGAATTCTCCTTTTGGGCTT96870.15085579948434633No Hit
AACGGAATCCCAAAAGCAGCTG96530.15032631696318727No Hit
ACCGGGTGCTGTAGGCTTA90990.1416988664713603No Hit
ACTGGACTTGGAGTCAGAAGGCA89360.13916046497286247No Hit
CACTAGATTGTGAGCTCCTGGAA88420.13769660153201096No Hit
ACCACAGGGTAGAACCACGGAC83850.13057973352702013No Hit
TGTAAACATCCCCGACTGGAAGC83360.12981665577593793No Hit
TTATAAAGCAATGAGACTGATT82030.12774544473728636No Hit
TGATGTAGTAGATTGTATAGTT80020.12461526865631663No Hit
TTCAAGTAATCCAGGATAGGC79160.12327598933809078No Hit
AGCAGCATTGTACAGGGCTATGT79020.12305796712349587No Hit
ACTGGACTTGGAGTCAGAAGGCT78590.12238832746438297No Hit
CTCCCACTGCTTCACTTGACTAGCCT77400.12053513864032626No Hit
CAAAGTGCTGTTCGTGCAGGTAG76970.11986549898121335No Hit
AGCTCGGTCTGAGGCCCCTCAGT76440.11904012916881834No Hit
TTCACAGTGGCTAAGTTCCGC75740.11795001809584381No Hit
TTCACAGTGGCTAAGTTCTGC75470.11752954668198219No Hit
ACTGGACTTGGAGTCAGAAGG75030.11684433400754107No Hit
TGAGGTAGTAGGTTGTGTGGTT74570.11612797530244352No Hit
GCGTTCGCGCTTTCCCCTG73480.11443051663166891No Hit
AACATTCAACGCTGTCGGTGAGA73210.1140100452178073No Hit
TCGTTTCCCGGCCAATGCACCA70870.11036595963100675No Hit
TATTGCACTTGTCCCGGCCTGTAAT70700.11010121837042722No Hit
AGCAGCATTGTACAGGGCTATG70240.10938485966532968No Hit
TTCACAGTGGCTAAGTTCTG70160.10926027554270401No Hit
CCAAGTCCTTCTGATCGAGGCCC70010.1090266803127809No Hit
TTCACAGTGGCTAAGTTCCG69300.10792099622447818No Hit
AACATTCAACGCTGTCGGTGA64830.1009598583727694No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)