FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947226

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947226
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7724344
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG5149916.667116327289412No Hit
CAACGGAATCCCAAAAGCAGCTG4915246.363310593106676No Hit
TAGCAGCACGTAAATATTGGCG2433313.1501833683222813No Hit
AACATTCAACGCTGTCGGTGAGT2383413.0855824132120473No Hit
TTCAAGTAATCCAGGATAGGCT2322513.0067407665945485No Hit
AAGCTGCCAGTTGAAGAACTGT2086912.701731046675291No Hit
AACATTCAACGCTGTCGGTGAG1638032.120607264513336No Hit
CCCCCACTGCTAAATTTGACTG1566582.0281075001320503No Hit
TAGCTTATCAGACTGATGTTGACT1547402.003276912576654No Hit
TAGCTTATCAGACTGATGTTGAC1350611.7485109415116675No Hit
TATTGCACTTGTCCCGGCCTGTAT1329201.7207933774052528No Hit
TATTGCACTTGTCCCGGCCTGTAA1249871.6180920994714891No Hit
TAGCTTATCAGACTGATGTTGA1214561.5723794797331658No Hit
TGAGGTAGTAGGTTGTATAGTT1172601.5180577146745406No Hit
TGAGGTAGTAGATTGTATAGTT937561.2137729754138344No Hit
TATTGCACTTGTCCCGGCCTGT812151.051416146147815No Hit
TGTAAACATCCTTGACTGGAAGCT794061.027996681659957No Hit
GGAATACCGGGTGCTGTAGGCTTT685950.8880365763099106No Hit
TATTGCACTTGTCCCGGCCTGTA563240.7291751895047658No Hit
TTCAAGTAATTCAGGATAGGTT558750.7233623981531635No Hit
CATAAAGTAGAAAGCACTACT554430.7177696902157646No Hit
AGCAGCATTGTACAGGGCTATGA517800.6703481874965692No Hit
GGAATACCGGGTGCTGTAGGCTT487990.6317559135118788No Hit
ACTGGACTTGGAGTCAGAAGGC487620.6312769084339072No Hit
TGTAAACATCCCCGACTGGAAGCT456970.5915971634613891No Hit
TGAGGTAGTAGTTTGTACAGTT380260.49228775932299235No Hit
CCCATAAAGTAGAAAGCACTAC356640.461709110831936No Hit
ACCGGGTGCTGTAGGCTTA322150.41705806991506333No Hit
TGAGAACTGAATTCCATGGGTT307460.3980402737112692No Hit
TTAATGCTAATCGTGATAGGGGTT304120.3937162819263358No Hit
TTCTCACTACTGCACTTGACTAG303350.3927194335208271No Hit
CAACGGAATCCCAAAAGCAGCTGT280240.36280103527238045No Hit
CATTGCACTTGTCTCGGTCTGA276350.3577650089120836No Hit
TCGTACGACTCTTAGCGG271130.3510071534877266No Hit
ACCGGGTGCTGTAGGCTT260390.3371030601433597No Hit
CCCCCACTGCTAAATTTGACTGGCTT245530.3178651805253624No Hit
CTCCCACTGCTTCACTTGACTAG242530.31398135556883533No Hit
ATCCCGGACGAGCCCCCA241190.31224658042158665No Hit
GCCTGTCTGAGCGTCGCT233760.30262764061258796No Hit
CGCCTGTCTGAGCGTCGCT228740.2961287068519993No Hit
ACCGGGTGCTGTAGGCTTT221750.2870793947032913No Hit
CCCATAAAGTAGAAAGCACT220740.28577184030126057No Hit
CTTCTCACTACTGCACTTGACTAG215660.2791952300415414No Hit
TGAGGTAGTAGTTTGTGCTGTT215070.2784314111334244No Hit
TAGCTTATCAGACTGATGTTGACA199730.25857211952238274No Hit
AGCTCGGTCTGAGGCCCCTCAGT190000.24597558058004668No Hit
TGTAAACATCCTACACTCTCAGCT189100.24481043309308856No Hit
CAAAGAATTCTCCTTTTGGGCT185090.23961905373453074No Hit
CGCGACCTCAGATCAGACGTGGCG183620.23771597950583245No Hit
CAACACCAGTCGATGGGCTGT181160.23453124304148026No Hit
TGTCAGTTTGTCAAATACCCCAA172510.22333288108349397No Hit
CACTAGATTGTGAGCTCCTGGA170570.2208213409449398No Hit
TATTGCACTTGTCCCGGCCTGTAG169730.2197338699571122No Hit
AACATTCAACGCTGTCGGTGAGTT169050.2188535363002994No Hit
CCCCCCACTGCTAAATTTGACTGGCT168060.21757187406464548No Hit
CACTAGATTGTGAGCTCCTGGAA164750.2132867205292773No Hit
CACCCGGCTGTGTGCACATGTGC156510.20261914798201633No Hit
TATTGCACTTGTCCCGGCCTGTT156360.20242495673418998No Hit
CGCGACCTCAGATCAGACGTGG152220.19706527829418266No Hit
TTCAAGTAATCCAGGATAGGCTT145900.188883353719099No Hit
ACCCCCCACTGCTAAATTTGACTG144790.187446338485184No Hit
ACTGGACTTGGAGTCAGAAGGCA143860.1862423527486606No Hit
TAGCAGCACGTAAATATTGGC142370.1843133863535855No Hit
CCCCCCACTGCTAAATTTGACTGG134990.17475917696052895No Hit
AACGGAATCCCAAAAGCAGCTG134180.17371054422226664No Hit
CAAAGAATTCTCCTTTTGGGCTT132140.17106954325182824No Hit
ACTGGACTTGGAGTCAGAAGGCT127210.16468712423993545No Hit
TTATAAAGCAATGAGACTGATT124810.16158006427471383No Hit
CTCCCACTGCTTCACTTGACTAGCCT123240.15954752921413132No Hit
TGTCAGTTTGTCAAATACCCCA122880.15908147021934807No Hit
TGTAAACATCCCCGACTGGAAGC120170.1555730816752853No Hit
TATTGCACTTGTCCCGGCCTGTTT116720.1511066829752792No Hit
ACTGGACTTGGAGTCAGAAGG115690.14977323640687157No Hit
GGAATACCGGGTGCTGTAGGCTTA114820.14864692716947875No Hit
AGCAGCATTGTACAGGGCTATGT114770.14858219675353662No Hit
TGAGGTAGTAGGTTGTATAGT112670.14586351928396768No Hit
TTCAAGTAATCCAGGATAGGC112560.145721112368895No Hit
TCGTTTCCCGGGCGGCGCACCA110320.14282118973468816No Hit
TGAGAACTGAATTCCATAGGCTGT107840.1396105611039591No Hit
TATTGCACTTGTCCCGGCCTGTAAT102790.13307278909380527No Hit
TTCACAGTGGCTAAGTTCCG100550.13017286645959839No Hit
TGAGGTAGTAGGTTGTGTGGTT98080.12697518391205775No Hit
CTACGCCTGTCTGAGCGTCGCT95040.12303957462277704No Hit
TGAATACCGGGTGCTGTAGGCTTT93630.1212141768932093No Hit
TTCACAGTGGCTAAGTTCCGC91020.11783524918103076No Hit
TGAGGTAGTAGATTGTATAGT89500.11586744453639042No Hit
CCCCCCACTGCTAAATTTGACTGT89390.11572503762131775No Hit
TACGCCTGTCTGAGCGTCGCT85160.1102488444326146No Hit
AGCAGCATTGTACAGGGCTATG84800.10978278543783135No Hit
CAACGGAATCCCAAAAGCAGCT80890.10472086691115776No Hit
GGAATACCGGGTGCTGTAGGCT80370.10404767058535974No Hit
TGAGGTAGTAAGTTGTATTGTT80300.10395704800304079No Hit
TAGCAGCACGTAAATATTGGCGT79660.10312849867898166No Hit
TTCACAGTGGCTAAGTTCTGC79020.10229994935492256No Hit
TATTGCACTTGTCCCGGCCTGTAAA78640.10180799819376249No Hit
CAAAGTGCTGTTCGTGCAGGTAG78550.10169148344506666No Hit
TTCACAGTGGCTAAGTTCTG77500.10033214471028219No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)