FastQCFastQC Report
Fri 9 Sep 2022
EGAF00003947227

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947227
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6562556
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG4596877.004694512321113No Hit
CCCCCCACTGCTAAATTTGACTG3214434.89813725018118No Hit
AAGCTGCCAGTTGAAGAACTGT2413993.678429563115347No Hit
AACATTCAACGCTGTCGGTGAGT1961872.9894906801557197No Hit
TAGCAGCACGTAAATATTGGCG1926312.9353044758779965No Hit
TTCAAGTAATCCAGGATAGGCT1919472.9248817076760947No Hit
CCCCCACTGCTAAATTTGACTG1498562.283500514128946No Hit
AACATTCAACGCTGTCGGTGAG1470182.2402551688701777No Hit
TAGCTTATCAGACTGATGTTGA1218631.856944154076552No Hit
TAGCTTATCAGACTGATGTTGAC1082981.650241156037373No Hit
TGAGGTAGTAGGTTGTATAGTT1076081.6397269600442268No Hit
TAGCTTATCAGACTGATGTTGACT866931.3210249177302258No Hit
CGCCTGTCTGAGCGTCGCT807191.229993313580867No Hit
TATTGCACTTGTCCCGGCCTGT787151.1994564313051195No Hit
ACCGGGTGCTGTAGGCTT711681.0844555078844278No Hit
TGAGGTAGTAGATTGTATAGTT655870.9994124240616005No Hit
CATAAAGTAGAAAGCACTACT640950.9766773799720718No Hit
TATTGCACTTGTCCCGGCCTGTAA546200.8322976596313998No Hit
TCGTACGACTCTTAGCGG526460.8022179163118761No Hit
TATTGCACTTGTCCCGGCCTGTAT511310.7791323990225759No Hit
TTCAAGTAATTCAGGATAGGTT485530.7398489247177471No Hit
ACCGGGTGCTGTAGGCTTT443570.6759104227072501No Hit
TGTAAACATCCTTGACTGGAAGCT434340.6618457808207656No Hit
AGCAGCATTGTACAGGGCTATGA375200.5717284545838542No Hit
CAACACCAGTCGATGGGCTGT357070.5441020236627314No Hit
CATTGCACTTGTCTCGGTCTGA356600.5433858392979808No Hit
TATTGCACTTGTCCCGGCCTGTA348080.5304030929412259No Hit
ACCGGGTGCTGTAGGCTTA330070.5029595176025927No Hit
ACTGGACTTGGAGTCAGAAGGC326670.49777860943205665No Hit
CCCATAAAGTAGAAAGCACT299460.4563161061025613No Hit
TGAGGTAGTAGTTTGTACAGTT298170.4543504085907991No Hit
TTAATGCTAATCGTGATAGGGGTT257230.39196617903146275No Hit
TGTAAACATCCCCGACTGGAAGCT234380.3571474285324194No Hit
TGAGGTAGTAGTTTGTGCTGTT227060.34599323800055953No Hit
CCCATAAAGTAGAAAGCACTAC226800.34559705090516557No Hit
CAACGGAATCCCAAAAGCAGCTGT207300.3158830187506209No Hit
CAACGGAATCCCAAAAGCAGCT187100.2851023290315542No Hit
ATCCCGGACGAGCCCCCA180690.2753347933335731No Hit
TGTCAGTTTGTCAAATACCCCAA176920.2695900804503611No Hit
TGAGAACTGAATTCCATGGGTT167390.25506829960765287No Hit
CTACGCCTGTCTGAGCGTCGCT159570.2431522108154201No Hit
TTCTCACTACTGCACTTGACTAG159160.2425274542419143No Hit
TACGCCTGTCTGAGCGTCGCT159080.24240555052025461No Hit
CGCGACCTCAGATCAGACGTGG154960.23612750885478156No Hit
ACGCCTGTCTGAGCGTCGCT143590.21880194241390094No Hit
AACGGAATCCCAAAAGCAGCTG141360.2154038761726376No Hit
GCCTGTCTGAGCGTCGCT140210.21365151017377987No Hit
CACCCGGCTGTGTGCACATGTGC138010.2102991578281389No Hit
TTATAAAGCAATGAGACTGATT138010.2102991578281389No Hit
TAGCAGCACGTAAATATTGGC128920.19644784745455884No Hit
AACATTCAACGCTGTCGGTGAGTT123510.18820410827732365No Hit
GCGTTCGCGCTTTCCCCTG115670.17625754355467596No Hit
TGTAAACATCCTACACTCTCAGCT115250.1756175490159627No Hit
TGATGTAGTAGGTTGTATAGTT112420.17130520486225184No Hit
TTCACAGTGGCTAAGTTCCG112260.1710613974189325No Hit
TTCACAGTGGCTAAGTTCCGC111630.1701014056108626No Hit
TGAGGTAGTAGGTTGTGTGGTT109800.16731285797789763No Hit
TGAGGTAGTAGGTTGTATAGT109620.16703857460416338No Hit
TGTCAGTTTGTCAAATACCCCA108360.16511859098802356No Hit
TAGCTTATCAGACTGATGTTGACA106420.16216242573777656No Hit
ACTGGACTTGGAGTCAGAAGG105060.16009006246956214No Hit
TATTGCACTTGTCCCGGCCTGTT104630.15943482996564143No Hit
CACTAGATTGTGAGCTCCTGGA102190.1557167664550215No Hit
ATCCCACCGCTGCCACCA101000.1539034485953339No Hit
TTCAAGTAATCCAGGATAGGCTT100390.15297393271767892No Hit
CTCCCACTGCTTCACTTGACTAG100220.15271488730915211No Hit
CCACCCTGAACGCGCCCGA99960.15231870021375818No Hit
AGCAGCATTGTACAGGGCTATG98010.1493472969983037No Hit
TTCACAGTGGCTAAGTTCTG95770.14593399279183294No Hit
TTCAAGTAATCCAGGATAGGC95420.14540066400957188No Hit
TCGTTTCCCGGCCAATGCACCA93810.142947351611171No Hit
CTTCTCACTACTGCACTTGACTAG93570.142581640446192No Hit
ACCACAGGGTAGAACCACGGAC87280.13299696033070044No Hit
TTCACAGTGGCTAAGTTCTGC86480.13177792311410372No Hit
AGCAGCATTGTACAGGGCTATGT82920.12635320750024837No Hit
TGAGGTAGTAAGTTGTATTGTT79720.12147705863386157No Hit
ACTGGACTTGGAGTCAGAAGGCT79080.12050182886058419No Hit
TAGCTTATCAGACTGATGTTG79040.12044087699975436No Hit
TGAGGTAGTAGATTGTATAGT77320.11781994698407144No Hit
ACTGGACTTGGAGTCAGAAGGCA76220.11614377081125098No Hit
TGAGAACTGAATTCCATAGGCTGT73860.1125476110222907No Hit
CAAAGAATTCTCCTTTTGGGCT72620.11065810333656581No Hit
CCCATAAAGTAGAAAGCACTA72550.1105514375801136No Hit
TGTAAACATCCCCGACTGGAAGC71160.10843336041627682No Hit
CCCCCACTGCTAAATTTGACTGGCTT69540.10596481005266851No Hit
CAAAGTGCTGTTCGTGCAGGT69300.10559909888768951No Hit
TAGCAGCACGTAAATATTGGCGT69270.10555338499206712No Hit
AATGACACGATCACTCCCGTTG67990.10360292544551238No Hit
CGCGACCTCAGATCAGACGTGGCG66930.10198770113352175No Hit
TGATGTAGTAGATTGTATAGTT66730.10168294182937257No Hit
TATTGCACTTGTCCCGGCCTGTAG66160.10081437781254741No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)