FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947228

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947228
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8903870
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG8740529.816540448142213No Hit
CAACGGAATCCCAAAAGCAGCTG5587346.275181466036678No Hit
TTCAAGTAATCCAGGATAGGCT2600732.920898440790353No Hit
CCCCCACTGCTAAATTTGACTG2593062.9122842090012546No Hit
TAGCAGCACGTAAATATTGGCG2509852.8188304636073975No Hit
AACATTCAACGCTGTCGGTGAGT2425212.7237706749986246No Hit
AAGCTGCCAGTTGAAGAACTGT2187862.457201194536758No Hit
AACATTCAACGCTGTCGGTGAG2017502.2658686616044483No Hit
TAGCTTATCAGACTGATGTTGACT1861382.090529174392708No Hit
TAGCTTATCAGACTGATGTTGAC1727691.9403809804051497No Hit
TATTGCACTTGTCCCGGCCTGTAT1430491.6065935374168763No Hit
TATTGCACTTGTCCCGGCCTGTAA1407761.5810653120497045No Hit
TGAGGTAGTAGGTTGTATAGTT1394811.5665210745439904No Hit
TAGCTTATCAGACTGATGTTGA1368141.536567807032223No Hit
TGAGGTAGTAGATTGTATAGTT1024361.1504660333091117No Hit
TGTAAACATCCTTGACTGGAAGCT860870.9668492464512621No Hit
TATTGCACTTGTCCCGGCCTGT794150.8919155378503953No Hit
TATTGCACTTGTCCCGGCCTGTA687000.7715746074459757No Hit
GGAATACCGGGTGCTGTAGGCTTT679430.7630726863712072No Hit
TTCAAGTAATTCAGGATAGGTT632670.7105561963505757No Hit
TTAATGCTAATCGTGATAGGGGTT601120.6751221659795124No Hit
CATAAAGTAGAAAGCACTACT522300.5865988609447352No Hit
AGCAGCATTGTACAGGGCTATGA517060.5807137795138518No Hit
GGAATACCGGGTGCTGTAGGCTT478920.5378784730684523No Hit
TGTAAACATCCCCGACTGGAAGCT464330.5214923398477291No Hit
ACTGGACTTGGAGTCAGAAGGC449330.5046457326982536No Hit
TGAGGTAGTAGTTTGTACAGTT406570.45662167125081565No Hit
CCCATAAAGTAGAAAGCACTAC392390.44069601195884484No Hit
ACCGGGTGCTGTAGGCTTA378890.42553406552431694No Hit
TGAGAACTGAATTCCATGGGTT337100.3785994180058783No Hit
CCCCCACTGCTAAATTTGACTGGCTT335790.3771281476481575No Hit
TTCTCACTACTGCACTTGACTAG322390.3620785119279594No Hit
CAACGGAATCCCAAAAGCAGCTGT291280.3271386486999473No Hit
ACCGGGTGCTGTAGGCTT285870.32106263905470317No Hit
GCCTGTCTGAGCGTCGCT281740.31642420655288095No Hit
CTCCCACTGCTTCACTTGACTAG281260.3158851151240977No Hit
CATTGCACTTGTCTCGGTCTGA280900.31548079655251027No Hit
TAGCTTATCAGACTGATGTTGACA257460.2891551651135967No Hit
CAAAGAATTCTCCTTTTGGGCT251060.28196727939648714No Hit
ACCGGGTGCTGTAGGCTTT248980.27963121653842654No Hit
TGAGGTAGTAGTTTGTGCTGTT241100.27078113224923545No Hit
TCGTACGACTCTTAGCGG239870.26939971046297845No Hit
CCCATAAAGTAGAAAGCACT228510.25664121331510903No Hit
CTTCTCACTACTGCACTTGACTAG222950.2503967375983701No Hit
TGTAAACATCCTACACTCTCAGCT222400.24977902866955604No Hit
AACATTCAACGCTGTCGGTGAGTT210190.23606589044988302No Hit
CAACACCAGTCGATGGGCTGT205940.23129268509086498No Hit
TATTGCACTTGTCCCGGCCTGTAG196080.2202188486579431No Hit
ACCCCCCACTGCTAAATTTGACTG195210.21924174544327352No Hit
CCCCCCACTGCTAAATTTGACTGGCT191840.21545687437035804No Hit
TGAGAACTGAATTCCATAGGCTGT186960.20997611151106205No Hit
AGCTCGGTCTGAGGCCCCTCAGT180760.2030128472226122No Hit
CACTAGATTGTGAGCTCCTGGAA180680.20292299865114832No Hit
CCCCCCACTGCTAAATTTGACTGG179560.20166511865065415No Hit
TATTGCACTTGTCCCGGCCTGTT174160.19560034007684296No Hit
CACTAGATTGTGAGCTCCTGGA173380.19472431650507027No Hit
ATCCCGGACGAGCCCCCA172370.19358997829033892No Hit
TGTCAGTTTGTCAAATACCCCAA171070.19212993900405106No Hit
TAGCAGCACGTAAATATTGGC169120.18993988007461923No Hit
CGCCTGTCTGAGCGTCGCT161960.18189843292860294No Hit
TTCACAGTGGCTAAGTTCCG160930.18074163257100564No Hit
CACCCGGCTGTGTGCACATGTGC152130.17085828970998004No Hit
AACGGAATCCCAAAAGCAGCTG149800.16824145006609487No Hit
CTCCCACTGCTTCACTTGACTAGCCT146730.16479351113616889No Hit
CGCGACCTCAGATCAGACGTGGCG136970.15383198541757687No Hit
TTCAAGTAATCCAGGATAGGCTT136600.15341643577455644No Hit
CAAAGAATTCTCCTTTTGGGCTT135410.1520799382740314No Hit
TGTCAGTTTGTCAAATACCCCA129680.1456445343429318No Hit
TGAGGTAGTAGGTTGTATAGT127860.14360047934212875No Hit
TTCAAGTAATCCAGGATAGGC124150.1394337518404918No Hit
TATTGCACTTGTCCCGGCCTGTTT122410.13747954541115268No Hit
TTATAAAGCAATGAGACTGATT121500.13645751791075117No Hit
AGCAGCATTGTACAGGGCTATGT117810.1323132525519802No Hit
CGCGACCTCAGATCAGACGTGG115480.12969641290809503No Hit
ACTGGACTTGGAGTCAGAAGGCT115220.12940440505083745No Hit
GGAATACCGGGTGCTGTAGGCTTA114060.12810160076461133No Hit
TGAGGTAGTAGGTTGTGTGGTT113230.1271694218356737No Hit
CCCCCCACTGCTAAATTTGACTGT112570.12642817112109678No Hit
TTCACAGTGGCTAAGTTCTG110900.12455258219178852No Hit
GACCCGCGGGCGCTCTCCAGTCCT110540.12414826362020111No Hit
ACTGGACTTGGAGTCAGAAGG109870.1233957818341912No Hit
ACTGGACTTGGAGTCAGAAGGCA109190.1226320689767483No Hit
TATTGCACTTGTCCCGGCCTGTAAT107540.12077894219030602No Hit
TGTAAACATCCCCGACTGGAAGC105390.11836426183221453No Hit
TCGTTTCCCGGGCGGCGCACCA100230.11256902897279497No Hit
TGAGGTAGTAAGTTGTATTGTT99570.11182777825821805No Hit
TATTGCACTTGTCCCGGCCTGTAAA97830.10987357182887891No Hit
GGGAATACCGGGTGCTGTAGGCTT94690.10634701539892204No Hit
CCCATAAAGTAGAAAGCACTA94570.10621224254172623No Hit
CAACGGAATCCCAAAAGCAGCT94270.10587531039873672No Hit
CTACGCCTGTCTGAGCGTCGCT94230.1058303861130048No Hit
AGCTACATCTGGCTACTGGGTCTC93970.10553837825574722No Hit
TGAGGTAGTAGATTGTATAGT90590.10174227611139876No Hit
GGGAATACCGGGTGCTGTAGGCTTT90420.10155134789703803No Hit
CAAAGTGCTGTTCGTGCAGGTAG89250.10023731253937895No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)