FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947229

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947229
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7405921
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG7114509.60650268886206No Hit
CAACGGAATCCCAAAAGCAGCTG4371615.902857996999968No Hit
CCCCCACTGCTAAATTTGACTG2373913.205421715948631No Hit
TAGCAGCACGTAAATATTGGCG2367253.1964289114075077No Hit
AACATTCAACGCTGTCGGTGAGT2345023.1664123881418664No Hit
TTCAAGTAATCCAGGATAGGCT2148182.900625053926446No Hit
AAGCTGCCAGTTGAAGAACTGT1850672.498905942961044No Hit
TAGCTTATCAGACTGATGTTGACT1761802.378907363446086No Hit
AACATTCAACGCTGTCGGTGAG1703032.299551939589958No Hit
TAGCTTATCAGACTGATGTTGAC1520822.0535190694040617No Hit
TAGCTTATCAGACTGATGTTGA1461881.9739340994860732No Hit
TATTGCACTTGTCCCGGCCTGTAT1160251.5666518721979346No Hit
TATTGCACTTGTCCCGGCCTGTAA1122011.5150175109888426No Hit
GGAATACCGGGTGCTGTAGGCTTT1008751.3620858229516626No Hit
TGAGGTAGTAGGTTGTATAGTT853301.1521862034445143No Hit
GGAATACCGGGTGCTGTAGGCTT745571.0067215137725611No Hit
TGAGGTAGTAGATTGTATAGTT719290.9712363931508317No Hit
TGTAAACATCCTTGACTGGAAGCT715500.9661188662422946No Hit
TATTGCACTTGTCCCGGCCTGT647280.8740033818886267No Hit
CATAAAGTAGAAAGCACTACT628670.8488748394696621No Hit
TATTGCACTTGTCCCGGCCTGTA574400.7755956348980768No Hit
TTCAAGTAATTCAGGATAGGTT541030.7305370932258122No Hit
AGCAGCATTGTACAGGGCTATGA454850.6141707425720583No Hit
TGTAAACATCCCCGACTGGAAGCT401250.5417962195383936No Hit
TTCTCACTACTGCACTTGACTAG362310.48921666866281727No Hit
ACTGGACTTGGAGTCAGAAGGC361320.48787990042021784No Hit
TGAATACCGGGTGCTGTAGGCTTT357710.4830054222830624No Hit
CCCATAAAGTAGAAAGCACTAC343660.4640341154057679No Hit
GCCTGTCTGAGCGTCGCT336430.4542716564219359No Hit
CCCCCACTGCTAAATTTGACTGGCTT289800.39130852192455196No Hit
CATTGCACTTGTCTCGGTCTGA271900.3671386718815931No Hit
TGAGGTAGTAGTTTGTACAGTT267660.3614135230446017No Hit
CTTCTCACTACTGCACTTGACTAG265170.35805134837382147No Hit
TGAATACCGGGTGCTGTAGGCTT264880.3576597697977065No Hit
CTCCCACTGCTTCACTTGACTAG260430.35165106406076974No Hit
TAGCTTATCAGACTGATGTTGACA222490.3004217841373139No Hit
TGTCAGTTTGTCAAATACCCCAA218520.29506120845739514No Hit
CCCATAAAGTAGAAAGCACT213920.2888499620776403No Hit
CAACGGAATCCCAAAAGCAGCTGT205180.2770485939561062No Hit
CAACACCAGTCGATGGGCTGT203260.2744560737280346No Hit
CAAAGAATTCTCCTTTTGGGCT194430.26253318122080965No Hit
AACATTCAACGCTGTCGGTGAGTT183180.24734263300945283No Hit
ACCGGGTGCTGTAGGCTTA176390.23817429324455391No Hit
GGAATACCGGGTGCTGTAGGCTTA173870.23477161044521No Hit
CACTAGATTGTGAGCTCCTGGAA157400.2125326478637836No Hit
CACTAGATTGTGAGCTCCTGGA157100.2121275665781474No Hit
TGAGGTAGTAGTTTGTGCTGTT155310.2097105815738515No Hit
CCCCCCACTGCTAAATTTGACTGGCT152700.20618637438881673No Hit
TCGTACGACTCTTAGCGG151740.20489011427478093No Hit
CTCCCACTGCTTCACTTGACTAGCCT150640.2034048162274483No Hit
TTCACAGTGGCTAAGTTCCG150220.20283770242755764No Hit
TGTAAACATCCTACACTCTCAGCT149650.20206804798484887No Hit
ACCGGGTGCTGTAGGCTT147710.1994485223377349No Hit
TATTGCACTTGTCCCGGCCTGTAG147560.19924598169491683No Hit
ACCCCCCACTGCTAAATTTGACTG147300.19889491124736544No Hit
TAGCAGCACGTAAATATTGGC147110.19863835976646252No Hit
TGTCAGTTTGTCAAATACCCCA145740.1967884885620573No Hit
CCCCCCACTGCTAAATTTGACTGG143570.1938584005959556No Hit
TATTGCACTTGTCCCGGCCTGTT142130.19191401042490192No Hit
TTATAAAGCAATGAGACTGATT140070.18913245226353345No Hit
CGCCTGTCTGAGCGTCGCT136980.18496011502148077No Hit
TTCAAGTAATCCAGGATAGGCTT133010.17959953934156198No Hit
TGATGTAGTAGGTTGTATAGTT128580.17361783902366767No Hit
CAAAGAATTCTCCTTTTGGGCTT123590.16687998697258585No Hit
TCCTGTCTGAGCGTCGCT123230.16639388942982244No Hit
GGAATACCGGGTGCTGTAGGCT121390.16390939087792053No Hit
CACCCGGCTGTGTGCACATGTGC119830.16180296819261236No Hit
ACCGGGTGCTGTAGGCTTT113820.15368783977036754No Hit
TGATGTAGTAGATTGTATAGTT110450.14913742666172106No Hit
TTCACAGTGGCTAAGTTCTG109820.1482867559618851No Hit
TGTAAACATCCCCGACTGGAAGC108870.1470039985573705No Hit
TTCAAGTAATCCAGGATAGGC108150.1460318034718437No Hit
ACTGGACTTGGAGTCAGAAGG105890.1429801911200511No Hit
ACTGGACTTGGAGTCAGAAGGCA105860.1429396829914875No Hit
ATCCCGGACGAGCCCCCA105550.1425210989963301No Hit
AGCAGCATTGTACAGGGCTATGT105020.14180545539170616No Hit
ACTGGACTTGGAGTCAGAAGGCT104480.14107630907756105No Hit
TATTGCACTTGTCCCGGCCTGTTT97650.1318539584745773No Hit
AACGGAATCCCAAAAGCAGCTG96890.13082775255096565No Hit
AGCTCGGTCTGAGGCCCCTCAGT93860.1267364315660402No Hit
CCCATAAAGTAGAAAGCACTA85890.11597477207763895No Hit
TTCACAGTGGCTAAGTTCCGC83980.1133957545590886No Hit
TATTGCACTTGTCCCGGCCTGTAAT81890.11057368826915652No Hit
CTACGCCTGTCTGAGCGTCGCT80260.10837274661719995No Hit
AGCAGCATTGTACAGGGCTATG80000.10802167616964858No Hit
TTCACAGTGGCTAAGTTCTGC78870.10649586999375231No Hit
TGAGGTAGTAGGTTGTATAGT78270.10568570742247993No Hit
TAGCAGCACGTAAATATTGGCGT77540.10470000962743188No Hit
CAAAGTGCTGTTCGTGCAGGTAG76040.10267460319925098No Hit
TATTGCACTTGTCCCGGCCTGTAAA76000.10262059236116616No Hit
AGAATTACTCTGAGACCT74300.10032513174256111No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)