FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947230

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947230
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8777059
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG7760958.84231266988179No Hit
CCCCCCACTGCTAAATTTGACTG6484267.387736598329806No Hit
AACATTCAACGCTGTCGGTGAGT3371523.841286699793177No Hit
CCCCCACTGCTAAATTTGACTG2516072.8666435989549575No Hit
TAGCTTATCAGACTGATGTTGAC2354862.682971596750119No Hit
TAGCTTATCAGACTGATGTTGACT2342722.6691400843950124No Hit
AACATTCAACGCTGTCGGTGAG2252172.565973408632664No Hit
TAGCAGCACGTAAATATTGGCG2097442.389684289464159No Hit
TTCAAGTAATCCAGGATAGGCT2067482.3555498487591344No Hit
TATTGCACTTGTCCCGGCCTGTAA1938672.2087922617359643No Hit
GGAATACCGGGTGCTGTAGGCTTT1695061.9312391542542895No Hit
AAGCTGCCAGTTGAAGAACTGT1668701.9012063152361172No Hit
TAGCTTATCAGACTGATGTTGA1646151.8755143380031967No Hit
TATTGCACTTGTCCCGGCCTGTAT1447411.6490831382129252No Hit
TGAGGTAGTAGGTTGTATAGTT1339921.5266161478463345No Hit
GGAATACCGGGTGCTGTAGGCTT1042371.1876073750899931No Hit
TGAGGTAGTAGATTGTATAGTT969131.104162567438592No Hit
TGTAAACATCCTTGACTGGAAGCT711380.8104992799980039No Hit
TATTGCACTTGTCCCGGCCTGT687830.78366796896318No Hit
TATTGCACTTGTCCCGGCCTGTA682350.7774244197287496No Hit
TGAATACCGGGTGCTGTAGGCTTT615810.7016131485501009No Hit
CATAAAGTAGAAAGCACTACT604990.6892855568135067No Hit
TTCAAGTAATTCAGGATAGGTT597980.6812988268621643No Hit
AGCAGCATTGTACAGGGCTATGA588980.6710448226450341No Hit
TGTAAACATCCCCGACTGGAAGCT559430.6373775087987902No Hit
ACTGGACTTGGAGTCAGAAGGC493550.5623181979293975No Hit
CATTGCACTTGTCTCGGTCTGA414170.47187788073430975No Hit
GCCTGTCTGAGCGTCGCT389360.4436110091090877No Hit
TGAATACCGGGTGCTGTAGGCTT380130.4330949581175198No Hit
TGTCAGTTTGTCAAATACCCCAA373990.4260994485738332No Hit
CAACGGAATCCCAAAAGCAGCTGT367330.4185114854531569No Hit
CAACACCAGTCGATGGGCTGT358290.4082119078839507No Hit
CAACGGAATCCCAAAAGCAGCT340860.3883533197167753No Hit
TGAGGTAGTAGTTTGTACAGTT325280.37060249908312115No Hit
CACCCGGCTGTGTGCACATGTGC316210.36026874149985777No Hit
TAGCTTATCAGACTGATGTTGACA302600.3447624084559532No Hit
TGATGTAGTAGGTTGTATAGTT262100.29861938947886757No Hit
CCCATAAAGTAGAAAGCACTAC255620.2912365064425339No Hit
GGAATACCGGGTGCTGTAGGCTTA250300.2851752506164081No Hit
AACATTCAACGCTGTCGGTGAGTT226670.25825279287743197No Hit
TATTGCACTTGTCCCGGCCTGTAG222230.2531941507969811No Hit
CCCCCACTGCTAAATTTGACTGGCTT218540.24899000906795773No Hit
CACTAGATTGTGAGCTCCTGGA217430.247725348547845No Hit
CACTAGATTGTGAGCTCCTGGAA211940.24147040597539562No Hit
TTATAAAGCAATGAGACTGATT207650.23658266396523026No Hit
CAAAGAATTCTCCTTTTGGGCT205850.23453186312180427No Hit
TGAGGTAGTAGTTTGTGCTGTT196370.22373097867976052No Hit
TATTGCACTTGTCCCGGCCTGTAAT193950.2209737908791544No Hit
TTCTCACTACTGCACTTGACTAG193650.2206319907385834No Hit
TGTCAGTTTGTCAAATACCCCA193210.2201306838657459No Hit
TGATGTAGTAGATTGTATAGTT187950.21413778806773429No Hit
CAAAGAATTCTCCTTTTGGGCTT181720.20703973848187643No Hit
CCCATAAAGTAGAAAGCACT173200.1973326144896599No Hit
TGTAAACATCCTACACTCTCAGCT169040.192592985873742No Hit
GGAATACCGGGTGCTGTAGGCT167980.19138529204372445No Hit
ACTGGACTTGGAGTCAGAAGGCA166840.1900864515095546No Hit
TAGCAGCACGTAAATATTGGC163270.18601902983675966No Hit
ACTGGACTTGGAGTCAGAAGGCT157460.1793995004477012No Hit
AACGGAATCCCAAAAGCAGCTG157310.17922860037741573No Hit
TATTGCACTTGTCCCGGCCTGTAAA153380.17475101853593555No Hit
TTCACAGTGGCTAAGTTCCGC151720.17285972442477598No Hit
CTTCTCACTACTGCACTTGACTAG148850.1695898364133134No Hit
TTCAAGTAATCCAGGATAGGCTT140900.16053213268818176No Hit
ATGCACCTGGGCAAGGATTCTGA134680.15344547644034295No Hit
ACCCCCCACTGCTAAATTTGACTG131990.15038066851322293No Hit
TGTAAACATCCCCGACTGGAAGC131040.14929830140141476No Hit
TCCTGTCTGAGCGTCGCT130600.14879699452857728No Hit
CGCGACCTCAGATCAGACGTGGCG129720.14779438078290233No Hit
CTCCCACTGCTTCACTTGACTAG129150.14714496051581744No Hit
TATTGCACTTGTCCCGGCCTGTT126330.14393203919445No Hit
TGAGGTAGTAAGTTGTATTGTT125140.14257623197018499No Hit
AGCAGCATTGTACAGGGCTATGT120130.13686816962264922No Hit
CGCGACCTCAGATCAGACGTGG119210.13581998252489813No Hit
TTCACAGTGGCTAAGTTCTGC117400.1337577883434531No Hit
TCGTACGACTCTTAGCGG114050.12994102010707687No Hit
ACTTGACTTGGAGTCAGAAGGC113640.1294738932482965No Hit
CAACGGAATCCCAAAAGCAGCTGA113160.12892701302338289No Hit
TAGCTTATCAGACTGATGTTGACC106110.12089470971996429No Hit
CTCCCACTGCTTCACTTGACTAGCCT104320.11885530221455728No Hit
CCCCCCACTGCTAAATTTGACTGG102520.11680450137113126No Hit
TCGTTTCCCGGCCAATGCACCA100570.11458280045741974No Hit
CAAAGTGCTGTTCGTGCAGGTAG99640.11352322002164963No Hit
TGAATACCGGGTGCTGTAGGCTTA95430.10872662471563652No Hit
CCCCCCACTGCTAAATTTGACTGGCT94730.10792909105430419No Hit
AATGCACCCGGGCAAGGATTCT89750.10225520872082551No Hit
CGCCTGTCTGAGCGTCGCT89240.1016741484818548No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)