FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947231

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947231
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6982395
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG4551166.518050038704485No Hit
CCCCCCACTGCTAAATTTGACTG2990854.2834156474963105No Hit
TAGCAGCACGTAAATATTGGCG2557503.6627833286429654No Hit
AAGCTGCCAGTTGAAGAACTGT2445663.502609061790403No Hit
TTCAAGTAATCCAGGATAGGCT2175923.1162946238360907No Hit
TAGCTTATCAGACTGATGTTGAC1809442.591431736531663No Hit
AACATTCAACGCTGTCGGTGAGT1743852.4974954868637482No Hit
GCCTGTCTGAGCGTCGCT1716862.4588411282948046No Hit
TAGCTTATCAGACTGATGTTGACT1373591.9672189843169856No Hit
TAGCTTATCAGACTGATGTTGA1140721.6337087775756025No Hit
TGTAAACATCCTTGACTGGAAGCT1017181.4567780825920047No Hit
TATTGCACTTGTCCCGGCCTGTAA936711.3415310935574398No Hit
CCCCCACTGCTAAATTTGACTG899051.2875954454023297No Hit
AACATTCAACGCTGTCGGTGAG854471.2237491577030517No Hit
GGAATACCGGGTGCTGTAGGCTTT815591.1680662580676116No Hit
TATTGCACTTGTCCCGGCCTGTAT766221.0973598600480208No Hit
TGAGGTAGTAGGTTGTATAGTT721451.0332414594132815No Hit
TCCTGTCTGAGCGTCGCT643930.9222193817450888No Hit
CATAAAGTAGAAAGCACTACT584520.8371339633463876No Hit
TGAGGTAGTAGATTGTATAGTT577750.8274381498038996No Hit
AGCAGCATTGTACAGGGCTATGA553340.7924787984638508No Hit
TTCAAGTAATTCAGGATAGGTT539570.7727577715096324No Hit
GGAATACCGGGTGCTGTAGGCTT504370.7223452697820734No Hit
TATTGCACTTGTCCCGGCCTGT451770.6470129518596412No Hit
TGTAAACATCCCCGACTGGAAGCT424710.6082583411565802No Hit
TGTCAGTTTGTCAAATACCCCAA365630.5236455399615748No Hit
CCCATAAAGTAGAAAGCACTAC359340.5146371696244626No Hit
TGTAAACATCCTACACTCTCAGCT350470.5019337920584556No Hit
TATTGCACTTGTCCCGGCCTGTA337410.4832296081788556No Hit
TGAATACCGGGTGCTGTAGGCTTT319620.45775124437961473No Hit
CAACGGAATCCCAAAAGCAGCTGT314860.45093409925963795No Hit
TTCTCACTACTGCACTTGACTAG301560.43188619377735005No Hit
CCCCCACTGCTAAATTTGACTGGCTT274840.39361852201143016No Hit
TAGCTTATCAGACTGATGTTGACA258370.37003062702697287No Hit
ACTGGACTTGGAGTCAGAAGGC256070.36673662833454707No Hit
CAAAGAATTCTCCTTTTGGGCT247590.3545917983729079No Hit
TGTCAGTTTGTCAAATACCCCA239570.34310576814975374No Hit
TGAGGTAGTAGTTTGTACAGTT231790.3319634595292876No Hit
CTTCTCACTACTGCACTTGACTAG224860.3220384982516744No Hit
CAAAGAATTCTCCTTTTGGGCTT205380.29413976150017296No Hit
TTAATGCTAATCGTGATAGGGGTT203650.291662101614131No Hit
TGAATACCGGGTGCTGTAGGCTT198790.28470173915970093No Hit
CGCCTGTCTGAGCGTCGCT194620.2787295763129986No Hit
CATTGCACTTGTCTCGGTCTGA193910.27771273323838025No Hit
TGATGTAGTAGGTTGTATAGTT192250.27533532548645556No Hit
CTCCCACTGCTTCACTTGACTAG187910.2691196931711827No Hit
CCCCCCACTGCTAAATTTGACTGGCT162550.2327997771538276No Hit
AACATTCAACGCTGTCGGTGAGTT157480.22553865829704564No Hit
TGATGTAGTAGATTGTATAGTT153650.22005343438748454No Hit
TGAGGTAGTAGTTTGTGCTGTT152000.2176903483690052No Hit
TGTAAACATCCCCGACTGGAAGC151190.2165302879599335No Hit
TGTAAACATCCTACACTCAGCT147530.211288533518943No Hit
CAACGGAATCCCAAAAGCAGCT146830.21028601217776996No Hit
TAGCAGCACGTAAATATTGGC139880.2003324074332661No Hit
CTACGCCTGTCTGAGCGTCGCT138520.19838465168470132No Hit
GGAATACCGGGTGCTGTAGGCTTA133390.19103760242724738No Hit
CTCCCACTGCTTCACTTGACTAGCCT130180.18644032599129667No Hit
TTCAAGTAATCCAGGATAGGCTT128930.18465010931063053No Hit
TAGCAGCACGTAAATATTGGCGT124950.17895005939938946No Hit
ATCACATTGCCAGGGATTTCCA119360.1709442104034504No Hit
CCCCCCACTGCTAAATTTGACTGG118970.17038566279908254No Hit
CCCATAAAGTAGAAAGCACT116390.16669065557018758No Hit
TTCACAGTGGCTAAGTTCCGC108650.1556056338835027No Hit
TTATAAAGCAATGAGACTGATT104410.14953321890268312No Hit
TGTCAGTTTGTCAAATACCCC104400.14951889716923777No Hit
CACCCGGCTGTGTGCACATGTGC101550.14543720313731892No Hit
TATTGCACTTGTCCCGGCCTGTAG101450.14529398580286562No Hit
AGCTCGGTCTGAGGCCCCTCAGT100700.14421985579446595No Hit
TTCACAGTGGCTAAGTTCCG98360.14086857016825888No Hit
TTCACAGTGGCTAAGTTCTGC94050.13469590305332196No Hit
ACCCCCCACTGCTAAATTTGACTG94010.13463861611954064No Hit
TAGCAGCACATCATGGTTTACA93400.13376499037937556No Hit
CGCGACCTCAGATCAGACGTGGCG92520.13250467783618658No Hit
CACTAGATTGTGAGCTCCTGGAA91330.13080039155619239No Hit
TGTAGTGTTTCCTACTTTATGGA90240.1292393226106515No Hit
TTCAAGTAATCCAGGATAGGC89810.12862348807250235No Hit
AGCAGCATTGTACAGGGCTATGT89210.12776418406578258No Hit
TATTGCACTTGTCCCGGCCTGTT87540.1253724545804126No Hit
ACTGGACTTGGAGTCAGAAGGCA86910.12447018537335684No Hit
ATCACATTGCCAGGGATTTCC86850.12438425497268486No Hit
TCGTTTCCCGGCCAATGCACCA86640.12408349857033296No Hit
TACGCCTGTCTGAGCGTCGCT86550.123954602969325No Hit
TATTGCACTTGTCCCGGCCTGTAAT86290.12358223789974644No Hit
TGTAAACATCCTTGACTGGAAGC85930.12306665549571458No Hit
CAACACCAGTCGATGGGCTGT82980.11884174412934242No Hit
CAAAGTGCTGTTCGTGCAGGTAG81790.11713745784934824No Hit
CACTAGATTGTGAGCTCCTGGA81260.11637840597674581No Hit
ATGCACCTGGGCAAGGATTCTGA80950.11593443223994059No Hit
GTCAGGATGGCCGAGCGGTCTAAGG76050.10891678285172923No Hit
AACGGAATCCCAAAAGCAGCTG75980.10881653071761192No Hit
TGAGAACTGAATTCCATGGGTT75950.10877356551727595No Hit
GGAATACCGGGTGCTGTAGGCT75520.1081577309791268No Hit
CAGTGCAATAGTATTGTCAAAGC73980.10595218402854607No Hit
AACCGGGCGGAAACACCA72760.1042049325482159No Hit
GGGCTACGCCTGTCTGAGCGTCGCT71740.10274411573679232No Hit
TATTGCACTTGTCCCGGCCTGTAAA71140.10188481173007258No Hit
ACTTGACTTGGAGTCAGAAGGC70290.10066746438721957No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)