FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947233

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947233
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7582502
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG7467969.848939044130816No Hit
CAACGGAATCCCAAAAGCAGCTG6865049.053792534443117No Hit
TTCAAGTAATCCAGGATAGGCT2428793.2031511498447345No Hit
TAGCAGCACGTAAATATTGGCG2329763.07254782128643No Hit
CCCCCACTGCTAAATTTGACTG2256862.976405413411035No Hit
TAGCTTATCAGACTGATGTTGACT1871862.468657443150031No Hit
TATTGCACTTGTCCCGGCCTGTAA1802832.377618891495182No Hit
AACATTCAACGCTGTCGGTGAGT1778362.3453472218009304No Hit
TATTGCACTTGTCCCGGCCTGTAT1725312.2753835079766547No Hit
AAGCTGCCAGTTGAAGAACTGT1608012.1206852302841463No Hit
AACATTCAACGCTGTCGGTGAG1509791.9911501507022353No Hit
TAGCTTATCAGACTGATGTTGAC1484641.9579816793981724No Hit
TAGCTTATCAGACTGATGTTGA1175451.5502139003721989No Hit
TGTAAACATCCTTGACTGGAAGCT794261.047490656777934No Hit
TGAGGTAGTAGGTTGTATAGTT786341.0370455556754221No Hit
TATTGCACTTGTCCCGGCCTGTA731450.9646552022010677No Hit
TTCAAGTAATTCAGGATAGGTT642810.8477544747103265No Hit
TATTGCACTTGTCCCGGCCTGT634170.8363598189621315No Hit
TGAGGTAGTAGATTGTATAGTT628460.8288293230915073No Hit
CCCCCACTGCTAAATTTGACTGGCTT504730.6656509948826917No Hit
CATAAAGTAGAAAGCACTACT484360.6387865113652459No Hit
TGTAAACATCCCCGACTGGAAGCT475810.6275105499477613No Hit
AGCAGCATTGTACAGGGCTATGA474290.625505934584653No Hit
CAAAGAATTCTCCTTTTGGGCT371320.48970643199302816No Hit
GCCTGTCTGAGCGTCGCT345320.4554169586767007No Hit
CAACGGAATCCCAAAAGCAGCTGT334510.4411604507324891No Hit
CCCATAAAGTAGAAAGCACTAC333720.44011857827403145No Hit
ACTGGACTTGGAGTCAGAAGGC298710.3939464836276997No Hit
CACTAGATTGTGAGCTCCTGGAA287800.37955809309380995No Hit
TTCTCACTACTGCACTTGACTAG285290.37624784009288753No Hit
CTCCCACTGCTTCACTTGACTAG276880.36515651430095236No Hit
TAGCTTATCAGACTGATGTTGACA258120.3404153404773253No Hit
CATTGCACTTGTCTCGGTCTGA243800.32152975363540953No Hit
CTTCTCACTACTGCACTTGACTAG232550.30669296229661397No Hit
TATTGCACTTGTCCCGGCCTGTAG230670.3042135696106641No Hit
CACTAGATTGTGAGCTCCTGGA214460.2828354018238307No Hit
CCCCCCACTGCTAAATTTGACTGGCT210540.27766560430844595No Hit
CAAAGAATTCTCCTTTTGGGCTT205420.270913215716923No Hit
TGAGGTAGTAGTTTGTACAGTT203390.2682359991464559No Hit
CAACGGAATCCCAAAAGCAGCT198530.2618265052880962No Hit
TGTAAACATCCTACACTCTCAGCT196150.25868769965375543No Hit
CTCCCACTGCTTCACTTGACTAGCCT191660.2527661713772051No Hit
CACCCGGCTGTGTGCACATGTGC188420.2484931754716319No Hit
ACCCCCCACTGCTAAATTTGACTG184070.24275628282063097No Hit
TATTGCACTTGTCCCGGCCTGTAAA182220.2403164549115846No Hit
CCCATAAAGTAGAAAGCACT171210.22579618178801666No Hit
TATTGCACTTGTCCCGGCCTGTTT170130.2243718498194923No Hit
TAGCAGCACGTAAATATTGGC168570.22231448142051263No Hit
TATTGCACTTGTCCCGGCCTGTT165690.218516262837781No Hit
TATTGCACTTGTCCCGGCCTGTAAT160100.21114402607477056No Hit
TCCTGTCTGAGCGTCGCT143830.18968672873413026No Hit
AACATTCAACGCTGTCGGTGAGTT140930.18586213363346293No Hit
TTCAAGTAATCCAGGATAGGCTT140590.1854137328285571No Hit
TGTCAGTTTGTCAAATACCCCAA133460.17601050418450268No Hit
TGTAAACATCCCCGACTGGAAGC132190.17433559529558976No Hit
CCCCCCACTGCTAAATTTGACTGG131300.17316184024745396No Hit
AGCTCGGTCTGAGGCCCCTCAGT130740.1724232977452561No Hit
TGATGTAGTAGGTTGTATAGTT130590.17222547386073886No Hit
CAACACCAGTCGATGGGCTGT128630.16964057510304645No Hit
ACTGGACTTGGAGTCAGAAGGCA128020.16883609130600954No Hit
TGAGGTAGTAGTTTGTGCTGTT125680.16575003870754007No Hit
TTTAAACATCCTTGACTGGAAGCT124000.1635344112009466No Hit
AACGGAATCCCAAAAGCAGCTG123000.16221558530416477No Hit
ATGCACCTGGGCAAGGATTCTGA122070.16098907722015768No Hit
TGTCAGTTTGTCAAATACCCCA119010.1569534699760053No Hit
TTATAAAGCAATGAGACTGATT110340.14551924945090683No Hit
TTCACAGTGGCTAAGTTCCGC106440.1403758284534577No Hit
TTCACAGTGGCTAAGTTCCG104880.13831846005447807No Hit
TGATGTAGTAGATTGTATAGTT104450.1377513649188619No Hit
ACTGGACTTGGAGTCAGAAGGCT101290.13358387508503128No Hit
TTCAAGTAATCCAGGATAGGC101130.1333728629415462No Hit
CGCCCCACTGCTAAATTTGACTG99690.13147375365018038No Hit
AGCAGCATTGTACAGGGCTATGT98530.12994391560991345No Hit
TCGTACGACTCTTAGCGG94340.1244180351023976No Hit
ATCACATTGCCAGGGATTTCCA92720.12228153714961106No Hit
ATCACATTGCCAGGGATTTCC89040.11742825784945392No Hit
TTCACAGTGGCTAAGTTCTGC85710.11303656761317044No Hit
CCCATAAAGTAGAAAGCACTA84640.11162542390361387No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC82320.10856574782308002No Hit
TTCAAGTAATTCAGGATAGGTTA82190.1083943004564984No Hit
CCCCCACTGCTAAATTTGACTGGCT81260.1071677923724913No Hit
ACCGGGTGCTGTAGGCTTA80670.10638968509339002No Hit
CCCGCCACTGCTAAATTTGACTG79630.10501810616073692No Hit
CTACGGGGATGATTTTACGAACTGAA77390.10206393615194563No Hit
ACTTGACTTGGAGTCAGAAGGC76200.10049453333477526No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)