FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947234

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947234
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8759893
Sequences flagged as poor quality0
Sequence length18-26
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG5746156.5596120865859895No Hit
CCCCCCACTGCTAAATTTGACTG5120695.845607931512405No Hit
AAGCTGCCAGTTGAAGAACTGT3386263.8656408246082457No Hit
TAGCTTATCAGACTGATGTTGAC3059953.4931362746097467No Hit
TAGCAGCACGTAAATATTGGCG2987103.4099731583479382No Hit
TAGCTTATCAGACTGATGTTGACT2893393.302996965830519No Hit
TTCAAGTAATCCAGGATAGGCT2853153.2570603316730007No Hit
AACATTCAACGCTGTCGGTGAGT2449042.7957419114594204No Hit
CCCCCACTGCTAAATTTGACTG1605231.8324767208914536No Hit
TGTAAACATCCTTGACTGGAAGCT1444611.6491183168561532No Hit
TATTGCACTTGTCCCGGCCTGTAA1436171.6394834959742088No Hit
TAGCTTATCAGACTGATGTTGA1427141.6291751508836925No Hit
GGAATACCGGGTGCTGTAGGCTTT1297511.4811938912952476No Hit
TATTGCACTTGTCCCGGCCTGTAT1210631.3820145976668892No Hit
AACATTCAACGCTGTCGGTGAG1190131.358612485335152No Hit
TGAGGTAGTAGGTTGTATAGTT1179871.346900013504731No Hit
AGCAGCATTGTACAGGGCTATGA819840.9359018426366623No Hit
TGAGGTAGTAGATTGTATAGTT794540.9070202113199328No Hit
GGAATACCGGGTGCTGTAGGCTT692250.7902493786168393No Hit
TGTAAACATCCCCGACTGGAAGCT682040.7785939851091789No Hit
TATTGCACTTGTCCCGGCCTGT635520.7254883136129631No Hit
TGAATACCGGGTGCTGTAGGCTTT571400.6522910725051093No Hit
CATAAAGTAGAAAGCACTACT566720.646948541494742No Hit
TTCAAGTAATTCAGGATAGGTT549850.627690315395405No Hit
ACTGGACTTGGAGTCAGAAGGC518170.5915254900944567No Hit
TATTGCACTTGTCCCGGCCTGTA510490.5827582597184692No Hit
TAGCTTATCAGACTGATGTTGACA478070.5457486752406678No Hit
TGTAAACATCCTACACTCTCAGCT435360.49699237193878965No Hit
CAACGGAATCCCAAAAGCAGCTGT417350.476432760080517No Hit
CCCATAAAGTAGAAAGCACTAC409200.46712899347058234No Hit
CAAAGAATTCTCCTTTTGGGCT316390.3611802107628484No Hit
TGAATACCGGGTGCTGTAGGCTT304600.34772114225596135No Hit
CAAAGAATTCTCCTTTTGGGCTT293730.3353123148878645No Hit
CCCCCACTGCTAAATTTGACTGGCTT279790.31939887850228305No Hit
CTTCTCACTACTGCACTTGACTAG274730.31362255223893715No Hit
TTCTCACTACTGCACTTGACTAG269630.307800563317383No Hit
TGAGGTAGTAGTTTGTACAGTT269500.30765215967820614No Hit
CGCCTGTCTGAGCGTCGCT267490.30535761110324067No Hit
CATTGCACTTGTCTCGGTCTGA261610.2986452003466253No Hit
AACATTCAACGCTGTCGGTGAGTT256130.2923894161720925No Hit
TGATGTAGTAGGTTGTATAGTT247760.2828345049420124No Hit
TTCAAGTAATCCAGGATAGGCTT221640.25301678913201336No Hit
CAACGGAATCCCAAAAGCAGCT219900.2510304634999537No Hit
GCCTGTCTGAGCGTCGCT182050.20782217317038004No Hit
CCCCCCACTGCTAAATTTGACTGGCT177540.20267370845739782No Hit
TTATAAAGCAATGAGACTGATT177130.20220566621076308No Hit
CCCATAAAGTAGAAAGCACT175700.2005732261798175No Hit
AGCAGCATTGTACAGGGCTATGT175370.20019650924959928No Hit
TGATGTAGTAGATTGTATAGTT171920.19625810497913618No Hit
TAGCAGCACGTAAATATTGGC166830.19044753172213405No Hit
GGAATACCGGGTGCTGTAGGCTTA159970.18261638583941606No Hit
TGAGGTAGTAGTTTGTGCTGTT159500.18207984960546894No Hit
CAACACCAGTCGATGGGCTGT158450.1808812048275019No Hit
CTCCCACTGCTTCACTTGACTAG155560.17758207777195453No Hit
ACTGGACTTGGAGTCAGAAGGCA155170.1771368668544239No Hit
TATTGCACTTGTCCCGGCCTGTAAT154850.17677156558875776No Hit
AGCTCGGTCTGAGGCCCCTCAGT153820.17559575213989487No Hit
TGTAAACATCCCCGACTGGAAGC153240.174933643595875No Hit
CACTAGATTGTGAGCTCCTGGAA142360.16251340056322605No Hit
TATTGCACTTGTCCCGGCCTGTAG139210.15891746622932493No Hit
TGTAAACATCCTACACTCAGCT136760.1561206284140685No Hit
ATCACATTGCCAGGGATTTCCA135540.15472791733871635No Hit
TATTGCACTTGTCCCGGCCTGTT133880.15283291702307322No Hit
TAGCAGCACGTAAATATTGGCGT131260.14984201291043167No Hit
CTCCCACTGCTTCACTTGACTAGCCT130640.1491342417082035No Hit
CGCGACCTCAGATCAGACGTGGCG129930.14832372952500675No Hit
CCCCCCACTGCTAAATTTGACTGG129550.1478899342720282No Hit
ATGCACCTGGGCAAGGATTCTGA126090.14394011433701304No Hit
CACTAGATTGTGAGCTCCTGGA125350.14309535516016006No Hit
TCGTACGACTCTTAGCGG124290.14188529471764094No Hit
CAAAGTGCTGTTCGTGCAGGTAG122550.13989896908558128No Hit
ACTTGACTTGGAGTCAGAAGGC121870.13912270389604076No Hit
CAGTGCAATAGTATTGTCAAAGC118640.1354354442457231No Hit
TAGCTTATCAGACTGATGTTGACC118320.13507014298005693No Hit
TACGCCTGTCTGAGCGTCGCT118060.13477333570170322No Hit
TTCACAGTGGCTAAGTTCTGC117520.1341568898158916No Hit
TATTGCACTTGTCCCGGCCTGTAAA116180.13262719076591462No Hit
TCGTTTCCCGGCCAATGCACCA114920.13118881703235416No Hit
TGTCAGTTTGTCAAATACCCCA114060.13020706988087638No Hit
CTACGCCTGTCTGAGCGTCGCT111410.12718191877457863No Hit
TTTAAACATCCTTGACTGGAAGCT109430.12492161719326936No Hit
ACTGGACTTGGAGTCAGAAGGCT107320.12251291197278324No Hit
TGTAAACATCCTTGACTGGAAGC107220.12239875532726256No Hit
ACCCCCCACTGCTAAATTTGACTG105100.11997863444222436No Hit
AACGGAATCCCAAAAGCAGCTG104910.11976173681573507No Hit
TATTGCACTTGTCCCGGCCTGTTT103830.11852884504411185No Hit
CCCCCCACTGCTAAATTTGACTGTA103810.1185060137150077No Hit
ACCACAGGGTAGAACCACGGAC103560.11822062210120603No Hit
GGAATACCGGGTGCTGTAGGCT102650.11718179662696794No Hit
ATCACATTGCCAGGGATTTCC100430.11464751909640905No Hit
CTCGTACGACTCTTAGCGG100310.11451053112178425No Hit
TGCAAGTAATCCAGGATAGGCT99310.11336896466657755No Hit
TTCAAGTAATCCAGGATAGGC97240.11100592210429967No Hit
AGCAGCATTGTACAGGGCTATG95470.10898534947858382No Hit
TTCACAGTGGCTAAGTTCCGC93880.10717025881480516No Hit
TGAGGTAGTAAGTTGTATTGTT92410.10549215612565131No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)