FastQCFastQC Report
Fri 9 Sep 2022
EGAF00003947235

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947235
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6612518
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG5769388.724936552157589No Hit
AAGCTGCCAGTTGAAGAACTGT3148064.760758307198558No Hit
CCCCCCACTGCTAAATTTGACTG2871974.343232033546071No Hit
TTCAAGTAATCCAGGATAGGCT2579053.9002540333349565No Hit
TAGCAGCACGTAAATATTGGCG2457093.7158159720699433No Hit
AACATTCAACGCTGTCGGTGAGT1861492.8151000874402157No Hit
TAGCTTATCAGACTGATGTTGAC1669382.524575358433807No Hit
TAGCTTATCAGACTGATGTTGACT1326982.006769584596972No Hit
AACATTCAACGCTGTCGGTGAG921431.393463125544611No Hit
CCCCCACTGCTAAATTTGACTG891931.3488507706141593No Hit
TAGCTTATCAGACTGATGTTGA887721.3424840582664577No Hit
TGAGGTAGTAGGTTGTATAGTT867621.3120871655850312No Hit
TGTAAACATCCTTGACTGGAAGCT855401.2936070646612985No Hit
TATTGCACTTGTCCCGGCCTGTAA803661.2153615309629404No Hit
TATTGCACTTGTCCCGGCCTGTAT691711.0460614247099214No Hit
TGAGGTAGTAGATTGTATAGTT596990.9028179583027223No Hit
GGAATACCGGGTGCTGTAGGCTTT583980.883143153636784No Hit
CATAAAGTAGAAAGCACTACT514230.7776613991825807No Hit
TATTGCACTTGTCCCGGCCTGT499670.7556425555287714No Hit
AGCAGCATTGTACAGGGCTATGA495240.7489431408731138No Hit
TTCAAGTAATTCAGGATAGGTT445030.6730114004982671No Hit
CCCATAAAGTAGAAAGCACTAC444300.6719074337491406No Hit
CAACGGAATCCCAAAAGCAGCTGT421510.6374424992113442No Hit
TGTAAACATCCCCGACTGGAAGCT405210.6127922827582473No Hit
TTAATGCTAATCGTGATAGGGGTT355250.5372386131878961No Hit
GGAATACCGGGTGCTGTAGGCTT340980.5156583316673014No Hit
ACTGGACTTGGAGTCAGAAGGC334200.5054050514493874No Hit
TATTGCACTTGTCCCGGCCTGTA329320.49802510934563804No Hit
TGTAAACATCCTACACTCTCAGCT299400.4527775954636343No Hit
TTCTCACTACTGCACTTGACTAG258020.39019931590356355No Hit
TAGCTTATCAGACTGATGTTGACA255000.38563222058525964No Hit
TGAATACCGGGTGCTGTAGGCTTT244010.36901222801964395No Hit
CCCCCACTGCTAAATTTGACTGGCTT233710.3534357108744354No Hit
TGAGGTAGTAGTTTGTACAGTT232970.3523166212931292No Hit
CGCCTGTCTGAGCGTCGCT200850.3037420843315663No Hit
CAAAGAATTCTCCTTTTGGGCT200210.3027742230720582No Hit
CTTCTCACTACTGCACTTGACTAG198370.2999916219509724No Hit
CATTGCACTTGTCTCGGTCTGA197840.29919011184544225No Hit
AACATTCAACGCTGTCGGTGAGTT172510.2608839779339731No Hit
CAAAGAATTCTCCTTTTGGGCTT164530.24881595785448146No Hit
TGTCAGTTTGTCAAATACCCCAA160680.24299366746525303No Hit
TGAGGTAGTAGTTTGTGCTGTT155400.23500881207431118No Hit
TTCAAGTAATCCAGGATAGGCTT151270.22876308238404794No Hit
CTACGCCTGTCTGAGCGTCGCT151090.2284908714048113No Hit
TGTCAGTTTGTCAAATACCCCA150440.22750788731312338No Hit
TGATGTAGTAGGTTGTATAGTT144030.21781415188586256No Hit
TAGCAGCACGTAAATATTGGC143490.21699751894815258No Hit
CCCATAAAGTAGAAAGCACT143100.21640772849313983No Hit
TGAATACCGGGTGCTGTAGGCTT139580.21108449156584524No Hit
CAACGGAATCCCAAAAGCAGCT138830.2099502791523592No Hit
CCCCCCACTGCTAAATTTGACTGGCT136070.2057763774707305No Hit
TTTAAACATCCTTGACTGGAAGCT129180.19535674609883863No Hit
CTCCCACTGCTTCACTTGACTAG126410.1911677215850301No Hit
TAGCAGCACGTAAATATTGGCGT122190.18478588640514854No Hit
TTCACAGTGGCTAAGTTCCGC119160.18020366825466486No Hit
TTCACAGTGGCTAAGTTCTGC118610.17937191248477508No Hit
AGCTCGGTCTGAGGCCCCTCAGT118300.17890310468720086No Hit
TGTAAACATCCTACACTCAGCT117810.17816208591038996No Hit
TGTAAACATCCCCGACTGGAAGC114590.1732925339484898No Hit
ATCACATTGCCAGGGATTTCCA114500.1731564284588715No Hit
TACGCCTGTCTGAGCGTCGCT109140.16505059041049114No Hit
GCCTGTCTGAGCGTCGCT108860.16462715110945633No Hit
CCCCCCACTGCTAAATTTGACTGG108390.16391637799700506No Hit
AACGGAATCCCAAAAGCAGCTG102480.15497878417873495No Hit
CAACACCAGTCGATGGGCTGT101650.15372358910781037No Hit
TGATGTAGTAGATTGTATAGTT101290.15317916714933708No Hit
TTCAAGTAATCCAGGATAGGC101040.1528010963448417No Hit
TTCACAGTGGCTAAGTTCCG100810.15245327120470598No Hit
ACTGGACTTGGAGTCAGAAGGCA100710.15230204288290786No Hit
ATCACATTGCCAGGGATTTCC100600.1521356917289299No Hit
TTATAAAGCAATGAGACTGATT97350.1472207712704903No Hit
TCGTTTCCCGGCCAATGCACCA97220.14702417445215274No Hit
CTCCCACTGCTTCACTTGACTAGCCT93320.14112626990202523No Hit
GTCAGGATGGCCGAGCGGTCTAAGG92060.13922079304736865No Hit
TATTGCACTTGTCCCGGCCTGTT91190.13790510664772482No Hit
CAAAGTGCTGTTCGTGCAGGTAG89310.13506201419791974No Hit
AGCAGCATTGTACAGGGCTATGT88570.13394292461661353No Hit
TAGCAGCACATCATGGTTTACA87460.13226429024465414No Hit
TGAGAACTGAATTCCATGGGTT83620.12645712268760553No Hit
ACTTGACTTGGAGTCAGAAGGC82450.12468775132256729No Hit
ATGCACCTGGGCAAGGATTCTGA81210.12281252013227034No Hit
CGCGACCTCAGATCAGACGTGGCG80030.12102802593505228No Hit
CACTAGATTGTGAGCTCCTGGA79350.1199996733468249No Hit
TATTGCACTTGTCCCGGCCTGTAG78230.11830591614268574No Hit
GGAATACCGGGTGCTGTAGGCTTA77670.11745903754061615No Hit
CACTAGATTGTGAGCTCCTGGAA76830.11618871963751176No Hit
ACGCCTGTCTGAGCGTCGCT75850.11470668208388998No Hit
AACCGGGCGGAAACACCA74160.11215092344550139No Hit
TATTGCACTTGTCCCGGCCTGTAAT72410.10950442781403393No Hit
ACTGGACTTGGAGTCAGAAGGCT71330.107871161938614No Hit
TGAGGTAGTAGGTTGTATAGT70800.10706965183308387No Hit
ACCCCCCACTGCTAAATTTGACTG70570.10672182669294814No Hit
TGTCAGTTTGTCAAATACCCC70100.10601105358049687No Hit
CCCCCCACTGCTAAATTTGACTGTA69660.10534564896458505No Hit
ACCACAGGGTAGAACCACGGAC69100.10449877036251545No Hit
TGAGGTAGTAGGTTGTGTGGTT69050.1044231562016164No Hit
AGAGGTAGTAGGTTGCATAGTT68940.10425680504763844No Hit
CAAAGTGCTGTTCGTGCAGGT66250.10018876319126845No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)