FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947237

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947237
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9881394
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG4880964.939545979038989No Hit
AAGCTGCCAGTTGAAGAACTGT4079184.128142243897976No Hit
CCCCCCACTGCTAAATTTGACTG3819143.8649809935723645No Hit
AACATTCAACGCTGTCGGTGAGT3639363.6830431010037654No Hit
TTCAAGTAATCCAGGATAGGCT3149263.1870604491633467No Hit
TAGCAGCACGTAAATATTGGCG3121903.1593720481138594No Hit
CGCCTGTCTGAGCGTCGCT1823771.8456606426178328No Hit
AACATTCAACGCTGTCGGTGAG1727061.7477898361304083No Hit
TAGCTTATCAGACTGATGTTGAC1673181.6932631165197947No Hit
CCCCCACTGCTAAATTTGACTG1498991.5169823205106487No Hit
TAGCTTATCAGACTGATGTTGA1410491.4274200583439949No Hit
TAGCTTATCAGACTGATGTTGACT1359641.3759597077092562No Hit
CATAAAGTAGAAAGCACTACT1153051.166890015720454No Hit
TGAGGTAGTAGGTTGTATAGTT1127431.1409624998254295No Hit
TATTGCACTTGTCCCGGCCTGT1118301.1317229127793103No Hit
TATTGCACTTGTCCCGGCCTGTAA932440.943632042199714No Hit
TGTAAACATCCTTGACTGGAAGCT890680.9013707984926014No Hit
TATTGCACTTGTCCCGGCCTGTAT834200.8442128711799165No Hit
TTAATGCTAATCGTGATAGGGGTT800590.8101994516158348No Hit
TGAGGTAGTAGATTGTATAGTT747700.7566746149379329No Hit
TCGTACGACTCTTAGCGG739330.7482041501431883No Hit
TATTGCACTTGTCCCGGCCTGTA635810.6434416034822618No Hit
TTCAAGTAATTCAGGATAGGTT626570.6340906961102857No Hit
AGCAGCATTGTACAGGGCTATGA558570.5652744946715008No Hit
ACTGGACTTGGAGTCAGAAGGC511920.5180645564785697No Hit
ATCCCGGACGAGCCCCCA467100.47270658370671187No Hit
TGTAAACATCCCCGACTGGAAGCT464250.46982237526405685No Hit
TACGCCTGTCTGAGCGTCGCT463260.46882049233134515No Hit
ACGCCTGTCTGAGCGTCGCT450740.4561502152429101No Hit
TTCACAGTGGCTAAGTTCCG385490.39011702195054665No Hit
CAACACCAGTCGATGGGCTGT382020.386605371671244No Hit
CATTGCACTTGTCTCGGTCTGA377070.38159595700768534No Hit
AACATTCAACGCTGTCGGTGAGTT364680.36905724030435383No Hit
GGAATACCGGGTGCTGTAGGCTTT362120.36646651272077607No Hit
TTCTCACTACTGCACTTGACTAG351590.3558101215273877No Hit
CCCATAAAGTAGAAAGCACTAC348690.352875312936616No Hit
CTACGCCTGTCTGAGCGTCGCT348060.35223775107034494No Hit
GCCTGTCTGAGCGTCGCT344640.348776700939159No Hit
ACCGGGTGCTGTAGGCTT333240.33723986716853915No Hit
GCGTTCGCGCTTTCCCCTG331620.33560042236955634No Hit
CCCATAAAGTAGAAAGCACT317190.32099721962306127No Hit
CAACGGAATCCCAAAAGCAGCTGT307970.3116665523103319No Hit
TGAGGTAGTAGTTTGTACAGTT304480.3081346619717825No Hit
TGTAAACATCCTACACTCTCAGCT271690.2749510848368155No Hit
GGAATACCGGGTGCTGTAGGCTT263570.2667336207826547No Hit
TGATGTAGTAGGTTGTATAGTT239900.24277951066418363No Hit
TGTCAGTTTGTCAAATACCCCAA233310.23611041114239548No Hit
ACCGGGTGCTGTAGGCTTT231260.2340358050696086No Hit
TGAATACCGGGTGCTGTAGGCTTT228540.23128315701205718No Hit
TTATAAAGCAATGAGACTGATT224120.22681010391853618No Hit
CTTCTCACTACTGCACTTGACTAG220960.22361217455755736No Hit
TGAGGTAGTAGTTTGTGCTGTT217140.2197463232414374No Hit
CAACGGAATCCCAAAAGCAGCT202730.20516336055418902No Hit
TCCTGTCTGAGCGTCGCT201430.20384775670315342No Hit
TCGTTCGCGCTTTCCCCTG190170.1924526033472605No Hit
TTCACAGTGGCTAAGTTCTG184760.18697766732102777No Hit
ACCGGGTGCTGTAGGCTTA177920.18005556705865589No Hit
TATTGCACTTGTCCCGGCCTGTT173900.1759873151500689No Hit
CCTCGACACAAGGGTTTGT170500.17254650507812969No Hit
ACTGGACTTGGAGTCAGAAGG167310.16931821562828078No Hit
TAGCTTATCAGACTGATGTTGACA164640.16661616771884616No Hit
TGAATACCGGGTGCTGTAGGCTT164410.1663834070375091No Hit
CTCCCACTGCTTCACTTGACTAG163900.1658672855267182No Hit
TTCAAGTAATCCAGGATAGGCTT163340.16530056386781056No Hit
TGATGTAGTAGATTGTATAGTT159460.16137399237395048No Hit
TCGTTTCCCGGCCAATGCACCA155460.1573259805246102No Hit
TGTCAGTTTGTCAAATACCCCA153810.15565617563675732No Hit
AGCTACATTGTCTGCTGGGTTT152990.15482633320764255No Hit
CAAAGAATTCTCCTTTTGGGCT151930.15375361006756738No Hit
CAAAGTGCTGTTCGTGCAGGT150700.15250884642389526No Hit
AACCGGGCGGAAACACCA148100.14987763872182405No Hit
ATCCCACCGCTGCCACCA145410.14715535075314273No Hit
ACCACAGGGTAGAACCACGGAC145100.14684162983481885No Hit
TTCAAGTAATCCAGGATAGGC145050.1467910296867021No Hit
TAGCAGCACGTAAATATTGGC144760.14649754882762495No Hit
ACTGGACTTGGAGTCAGAAGGCA141320.14301625863719228No Hit
AGCAGCATTGTACAGGGCTATG138230.1398891694835769No Hit
TACCTGGTTGATCCTGCC135560.13718712157414228No Hit
CAAAGAATTCTCCTTTTGGGCTT134990.13661027988561128No Hit
TTCACAGTGGCTAAGTTCTGC133710.13531491609382237No Hit
TGTAAACATCCTACACTCAGCT132540.13413087262789036No Hit
CTCCTGTCTGAGCGTCGCT132530.134120752598267No Hit
TGTAAACATCCCCGACTGGAAGC132330.1339183520058No Hit
TGAGAACTGAATTCCATAGGCTGT124380.12587292845523618No Hit
AACGGAATCCCAAAAGCAGCTG123880.12536692697406865No Hit
TGAGGTAGTAGGTTGTGTGGTT123810.1252960867667052No Hit
TAGCAGCACGTAAATATTGGCGT122520.12399060294529295No Hit
CCAAGTCCTTCTGATCGAGGCCC122270.12373760220470917No Hit
TGAGGTAGTAGGTTGTATAGT120420.1218653967243893No Hit
CTCGACACAAGGGTTTGT119720.12115699465075475No Hit
CAAAGTGCTGTTCGTGCAGGTAG119060.1204890726956136No Hit
TGCAAGTAATCCAGGATAGGCT116890.11829302626734647No Hit
AACATTCAACGCTGTCGGTGA116780.11818170594148963No Hit
TTAGGTAGTAGGTTGTATAGTT113560.11492305640277069No Hit
ACTTGACTTGGAGTCAGAAGGC113390.11475101589917373No Hit
AATGACACGATCACTCCCGTTG112400.11374913296646202No Hit
AGCAGCATTGTACAGGGCTATGT112130.11347589216663155No Hit
CTGCGTTCGCGCTTTCCCCTG110640.11196800775275229No Hit
CACTAGATTGTGAGCTCCTGGA110490.11181620730840204No Hit
CACCCGTAGAACCGACCTTGCG109410.11072324410908016No Hit
ACTGGACTTGGAGTCAGAAGGCT108510.10981244144297858No Hit
TTTAAACATCCTTGACTGGAAGCT108310.10961004085051158No Hit
AGCTCGGTCTGAGGCCCCTCAGT107190.1084765975326963No Hit
CGCGACCTCAGATCAGACGTGG105870.10714075362241401No Hit
TTCACAGTGGCTAAGTTCCGC105320.1065841519931297No Hit
CCCCCCACTGCTAAATTTGACTGG103470.10471194651280984No Hit
TATTGCACTTGTCCCGGCCTGTAG103220.10445894577222606No Hit
AGCTACATCTGGCTACTGGGTCTC103110.1043476254463692No Hit
TTAATGCTAATCGTGATAGGGGT100120.10132173658898734No Hit
TGAGAACTGAATTCCATGGGTT99670.10086633525593655No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)