FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947238

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947238
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7318607
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG6881659.40295058882107No Hit
CCCCCCACTGCTAAATTTGACTG5059596.91332380601937No Hit
AACATTCAACGCTGTCGGTGAGT2741163.7454668627513406No Hit
CCCCCACTGCTAAATTTGACTG2238883.05916139505783No Hit
AACATTCAACGCTGTCGGTGAG1981682.7077283969476706No Hit
TAGCAGCACGTAAATATTGGCG1922912.6274262301555473No Hit
TTCAAGTAATCCAGGATAGGCT1902282.5992378057736945No Hit
TAGCTTATCAGACTGATGTTGAC1678202.293059321261546No Hit
TAGCTTATCAGACTGATGTTGACT1518482.074821069091427No Hit
AAGCTGCCAGTTGAAGAACTGT1406621.92197777527882No Hit
TGAGGTAGTAGGTTGTATAGTT1324591.8098936040697362No Hit
TATTGCACTTGTCCCGGCCTGTAA1297601.7730150013520332No Hit
TAGCTTATCAGACTGATGTTGA1225181.6740617442636285No Hit
TATTGCACTTGTCCCGGCCTGTAT995401.3600948923750107No Hit
TGAGGTAGTAGATTGTATAGTT860991.1764397241168982No Hit
GCCTGTCTGAGCGTCGCT713190.9744887244252901No Hit
TATTGCACTTGTCCCGGCCTGT648670.8863298712446235No Hit
TATTGCACTTGTCCCGGCCTGTA580460.793129074972874No Hit
AGCAGCATTGTACAGGGCTATGA551200.7531487891069981No Hit
TGTAAACATCCTTGACTGGAAGCT487900.6666569198209441No Hit
ACTGGACTTGGAGTCAGAAGGC481230.6575431636102335No Hit
TTCAAGTAATTCAGGATAGGTT467640.6389740561284408No Hit
CATAAAGTAGAAAGCACTACT455230.6220172773316015No Hit
CATTGCACTTGTCTCGGTCTGA428750.5858355285370563No Hit
TGTAAACATCCCCGACTGGAAGCT383080.5234329429083977No Hit
CAACGGAATCCCAAAAGCAGCT360940.4931812843619011No Hit
CAACACCAGTCGATGGGCTGT314100.42918003385070413No Hit
GGAATACCGGGTGCTGTAGGCTTT310120.42374184048959046No Hit
CAACGGAATCCCAAAAGCAGCTGT305130.41692360308457604No Hit
TGATGTAGTAGGTTGTATAGTT285850.39057979202872894No Hit
TCCTGTCTGAGCGTCGCT282590.3861253924414851No Hit
CCCCCACTGCTAAATTTGACTGGCTT277810.37959409488718276No Hit
TGAGGTAGTAGTTTGTACAGTT252130.34450545028582624No Hit
TTCTCACTACTGCACTTGACTAG241530.3300218197260763No Hit
TAGCTTATCAGACTGATGTTGACA240360.32842315484353785No Hit
CCCATAAAGTAGAAAGCACTAC212690.2906154135616245No Hit
AACATTCAACGCTGTCGGTGAGTT207540.2835785553179724No Hit
GGAATACCGGGTGCTGTAGGCTT205820.28122838130261674No Hit
TCGTTTCCCGGCCAATGCACCA204850.27990299246837547No Hit
CCCATAAAGTAGAAAGCACT197780.27024268416107056No Hit
TGAGGTAGTAGTTTGTGCTGTT188560.257644658334571No Hit
CACTAGATTGTGAGCTCCTGGA185890.25399642308980386No Hit
TCGTACGACTCTTAGCGG183550.250799093324727No Hit
TGATGTAGTAGATTGTATAGTT183330.25049848967160004No Hit
TTATAAAGCAATGAGACTGATT178400.24376223508107484No Hit
CTTCTCACTACTGCACTTGACTAG172400.2355639536321598No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC162400.2219001512173013No Hit
AACGGAATCCCAAAAGCAGCTG159080.2173637688155683No Hit
CACTAGATTGTGAGCTCCTGGAA156230.21346958512733363No Hit
ACTGGACTTGGAGTCAGAAGGCA150280.20533962269049288No Hit
TATTGCACTTGTCCCGGCCTGTAG147910.20210130151817143No Hit
TAGCAGCACGTAAATATTGGC144100.19689539279811036No Hit
CACCCGGCTGTGTGCACATGTGC142780.19509177087934904No Hit
CAAAGAATTCTCCTTTTGGGCT142290.19442224456102097No Hit
ACTGGACTTGGAGTCAGAAGGCT139400.19047340566312687No Hit
TGAATACCGGGTGCTGTAGGCTTT138930.18983120694962854No Hit
CTCCCACTGCTTCACTTGACTAG130510.17832628531631772No Hit
TGTAAACATCCTACACTCTCAGCT126060.1722458932417057No Hit
ACTTGACTTGGAGTCAGAAGGC117630.16072730780598002No Hit
CCCCCCACTGCTAAATTTGACTGGCT117370.16037204894319368No Hit
TATTGCACTTGTCCCGGCCTGTT116920.1597571778345251No Hit
TTCACAGTGGCTAAGTTCTG116650.1593882551693239No Hit
AGCAGCATTGTACAGGGCTATGT115870.15832247858096493No Hit
CAACGGAATCCCAAAAGCAGCTGA114600.1565871756742779No Hit
TTCAAGTAATCCAGGATAGGCTT113530.15512514881588804No Hit
CAAAGAATTCTCCTTTTGGGCTT113310.15482454516276115No Hit
ACTGGACTTGGAGTCAGAAGG110750.1513266117445574No Hit
TTCACAGTGGCTAAGTTCTGC105300.14387983942845955No Hit
CTCCCACTGCTTCACTTGACTAGCCT105170.14370220999706637No Hit
AGCAGCATTGTACAGGGCTATG104430.14269108861836685No Hit
AATGCACCCGGGCAAGGATTCT103410.14129738077205128No Hit
TGAGGTAGTAGGTTGTATAGT100960.13794974918041097No Hit
TGAGGTAGTAAGTTGTATTGTT99890.1364877223220211No Hit
AGAATTACTCTGAGACCT97580.1333313839641888No Hit
TATTGCACTTGTCCCGGCCTGTAAT97310.13296246129898762No Hit
TATTGCACTTGTCCCGGCCTGTAAA95840.13095388234400343No Hit
CGCCTGTCTGAGCGTCGCT95590.13061228728363197No Hit
TTAATGCTAATCGTGATAGGGGTT94950.12973780392908105No Hit
ACCGGGTGCTGTAGGCTT93890.12828944087310604No Hit
TGTAAACATCCCCGACTGGAAGC92110.12585728404326124No Hit
TCAGTGCACTACAGAACTTTGT91750.12536538715632634No Hit
ATGCACCTGGGCAAGGATTCTGA91640.1252150853297629No Hit
TTCACAGTGGCTAAGTTCCGC90620.12382137748344732No Hit
TGAATACCGGGTGCTGTAGGCTT89070.12170348810914428No Hit
TTCAAGTAATCCAGGATAGGC87010.11888874481168342No Hit
ACCACAGGGTAGAACCACGGAC84890.11599201869973344No Hit
TGAGGTAGTAGGTTGTGTGGTT81070.11077244617725751No Hit
AACATTCAACGCTGTCGGTGA81050.11074511857242779No Hit
CCCATAAAGTAGAAAGCACTA79900.10917378129471907No Hit
TGAGGTAGTAGATTGTATAGT79160.10816265991601955No Hit
TTCACAGTGGCTAAGTTCCG78170.10680994347694855No Hit
CAAAGTGCTGTTCGTGCAGGTAG75890.10369459652636083No Hit
TGTCAGTTTGTCAAATACCCCAA75720.10346231188530823No Hit
ACCGGGTGCTGTAGGCTTT74680.10204127643416296No Hit
TGTCAGTTTGTCAAATACCCCA74100.10124877589410117No Hit
GTCAGGATGGCCGAGCGGTCTAAGG73890.10096183604338914No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)