FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947239

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947239
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9846569
Sequences flagged as poor quality0
Sequence length18-26
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG8908969.04778100879606No Hit
CAACGGAATCCCAAAAGCAGCTG5709785.798750813608273No Hit
AACATTCAACGCTGTCGGTGAGT5099115.178565244401375No Hit
TTCAAGTAATCCAGGATAGGCT2661662.7031344623695825No Hit
CCCCCACTGCTAAATTTGACTG2607182.6478055452615017No Hit
AACATTCAACGCTGTCGGTGAG2531502.5709462859601144No Hit
AAGCTGCCAGTTGAAGAACTGT2489782.5285761974551746No Hit
TAGCAGCACGTAAATATTGGCG2117192.1501804334078196No Hit
TCGTACGACTCTTAGCGG2079732.112136724985119No Hit
CGCCTGTCTGAGCGTCGCT1211461.230337186485973No Hit
TATTGCACTTGTCCCGGCCTGTAA1197251.2159057637233843No Hit
TGAGGTAGTAGGTTGTATAGTT1128281.1458610608426143No Hit
TATTGCACTTGTCCCGGCCTGT1109731.1270220114234715No Hit
TATTGCACTTGTCCCGGCCTGTAT1066601.0832199520462407No Hit
TAGCTTATCAGACTGATGTTGACT732560.7439748809966192No Hit
ACCGGGTGCTGTAGGCTT679480.6900677789390396No Hit
TAGCTTATCAGACTGATGTTGAC676880.6874272652738228No Hit
TAGCTTATCAGACTGATGTTGA674000.6845023885985058No Hit
TGAGGTAGTAGATTGTATAGTT664330.6746817089282572No Hit
CTCCCACTGCTTCACTTGACTAG647810.6579042913323413No Hit
CATAAAGTAGAAAGCACTACT509070.5170024198276577No Hit
TATTGCACTTGTCCCGGCCTGTA488590.4962032968031809No Hit
ACCGGGTGCTGTAGGCTTA473230.48060395453482324No Hit
ACTGGACTTGGAGTCAGAAGGC461040.46822400777367224No Hit
TTCTCACTACTGCACTTGACTAG405010.4113209382882505No Hit
AGCAGCATTGTACAGGGCTATGA404260.4105592516540533No Hit
TGTAAACATCCTTGACTGGAAGCT400660.40690315580990694No Hit
ACCGGGTGCTGTAGGCTTT400480.40672035101769966No Hit
CAACGGAATCCCAAAAGCAGCTGT400420.4066594160869639No Hit
GCCTGTCTGAGCGTCGCT393820.39995657370602894No Hit
AGCTCGGTCTGAGGCCCCTCAGT355850.36139491837207455No Hit
CTTCTCACTACTGCACTTGACTAG355260.36079572488650613No Hit
TGTAAACATCCCCGACTGGAAGCT335910.34114420972421966No Hit
ATCCCGGACGAGCCCCCA328300.3334156293425659No Hit
TTCAAGTAATTCAGGATAGGTT321030.3260323469017482No Hit
TACGCCTGTCTGAGCGTCGCT309690.3145156449926873No Hit
CCCATAAAGTAGAAAGCACTAC307210.3119970011889421No Hit
CCCATAAAGTAGAAAGCACT299990.30466449785707084No Hit
AACATTCAACGCTGTCGGTGAGTT297940.30258255439026527No Hit
CTACGCCTGTCTGAGCGTCGCT276500.2808084724740161No Hit
CCCCCCACTGCTAAATTTGACTGG270960.2751821472027465No Hit
TGAGGTAGTAGTTTGTACAGTT265220.26935270549569096No Hit
CATTGCACTTGTCTCGGTCTGA247940.2518034454437886No Hit
CAACACCAGTCGATGGGCTGT246110.24994493005634755No Hit
ACGCCTGTCTGAGCGTCGCT242140.2459130688059973No Hit
CACCCGGCTGTGTGCACATGTGC232590.23621425899722023No Hit
TGAGGTAGTAGGTTGTGTGGTT220660.22409836360259092No Hit
AACCGGGCGGAAACACCA215530.2188884270246824No Hit
CGCGACCTCAGATCAGACGTGGCG214740.2180861171033281No Hit
CCCCCCACTGCTAAATTTGACTGT204230.20741234840277867No Hit
AACATTCAACGCTGTCGGTGA199380.20248677483497043No Hit
GGAATACCGGGTGCTGTAGGCTTT195680.19872912077293114No Hit
TGAGGTAGTAGTTTGTGCTGTT193110.19611907457308225No Hit
CCCCCCACTGCTAAATTTGACTGGCT191490.19447383144321642No Hit
GACCCGCGGGCGCTCTCCAGTCCT186980.1898935558162442No Hit
CGCGACCTCAGATCAGACGTGG180550.18336336240572731No Hit
GGAATACCGGGTGCTGTAGGCTT177980.18075331620587842No Hit
CACTAGATTGTGAGCTCCTGGA175810.17854950287760132No Hit
GCGTTCGCGCTTTCCCCTG174830.17755423234225037No Hit
CTCCCACTGCTTCACTTGACTAGCCT172830.17552306798439132No Hit
TGTAAACATCCTACACTCTCAGCT164230.16678906124559734No Hit
AACGGAATCCCAAAAGCAGCTG162780.1653164670861495No Hit
ACCCCCCACTGCTAAATTTGACTG161640.16415870340216984No Hit
GCCCCCACTGCTAAATTTGACTG151160.15351540216698834No Hit
TATTGCACTTGTCCCGGCCTGTT151070.15342399977088467No Hit
AACATTCAACGCTGTCGGTGAGA145120.14738128580625393No Hit
TATTGCACTTGTCCCGGCCTGTAG139670.14184636293108796No Hit
CCCCCCACTGCTAAATTTGACTGTA138650.14081046910857986No Hit
TCCCACTGCTTCACTTGACTAG136340.13846447427525263No Hit
CCTCGACACAAGGGTTTGT135640.13775356675000194No Hit
CGCGACCTCAGATCAGACGTGGC132840.13490993664899925No Hit
TTCAAGTAATCCAGGATAGGCTT132070.13412793837122353No Hit
CGCGACCTCAGATCAGAC124920.12686652579187735No Hit
TTCAAGTAATCCAGGATAGGC123720.1256478271771619No Hit
ATCCCACCGCTGCCACCA123420.12534315252348305No Hit
AATGACACGATCACTCCCGTTG121960.12386040254224594No Hit
CAAAGTGCTGTTCGTGCAGGT116840.11866062178612673No Hit
ACTGGACTTGGAGTCAGAAGGCT114810.11659898996289977No Hit
ACCACAGGGTAGAACCACGGAC109840.11155154653361998No Hit
CCCCCACTGCTAAATTTGACTGGCTT108430.11011957566132934No Hit
TCCTGTCTGAGCGTCGCT107400.10907352601703194No Hit
TGAGGTAGTAGGTTGTATAGT106400.10805794383810238No Hit
CACTAGATTGTGAGCTCCTGGAA104750.10638223324286866No Hit
ACTGGACTTGGAGTCAGAAGG103760.10537680688572841No Hit
TTCACCACCTTCTCCACCCAGC102190.10378234286480906No Hit
AGAGGTAGTAGGTTGCATAGTT101710.10329486341892288No Hit
AACATTCATTGCTGTCGGTGGGT100870.10244177438862208No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)