FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947241

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947241
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6385651
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG69621110.902741161394507No Hit
CAACGGAATCCCAAAAGCAGCTG5210678.159966775509654No Hit
AACATTCAACGCTGTCGGTGAGT3670365.74782430170393No Hit
AACATTCAACGCTGTCGGTGAG2254343.5303213407685448No Hit
CCCCCACTGCTAAATTTGACTG1946733.0486006830000574No Hit
AAGCTGCCAGTTGAAGAACTGT1594792.4974587555755865No Hit
TAGCAGCACGTAAATATTGGCG1520162.3805873512348232No Hit
TTCAAGTAATCCAGGATAGGCT1476722.312559831409515No Hit
TATTGCACTTGTCCCGGCCTGTAA1231961.9292629678634174No Hit
TATTGCACTTGTCCCGGCCTGTAT1016571.5919598487295972No Hit
TAGCTTATCAGACTGATGTTGACT827171.2953573566735794No Hit
TAGCTTATCAGACTGATGTTGAC699031.0946887012772857No Hit
TATTGCACTTGTCCCGGCCTGT673711.0550373016001031No Hit
TAGCTTATCAGACTGATGTTGA556340.871234585166023No Hit
TGAGGTAGTAGGTTGTATAGTT529470.829155868368002No Hit
TATTGCACTTGTCCCGGCCTGTA455850.7138661351833979No Hit
TTAATGCTAATCGTGATAGGGGTT408580.6398407930530498No Hit
CGCCTGTCTGAGCGTCGCT399790.6260755559613265No Hit
TGTAAACATCCTTGACTGGAAGCT371840.5823055472339469No Hit
ACTGGACTTGGAGTCAGAAGGC353570.5536945254289657No Hit
AGCAGCATTGTACAGGGCTATGA339370.5314571685799929No Hit
TGTAAACATCCCCGACTGGAAGCT305290.47808751214245815No Hit
CAACGGAATCCCAAAAGCAGCTGT304670.4771165852941227No Hit
TGAGGTAGTAGATTGTATAGTT288180.4512930631504916No Hit
CATAAAGTAGAAAGCACTACT256710.4020106955422399No Hit
AACATTCAACGCTGTCGGTGAGTT223840.35053591247000504No Hit
TTCAAGTAATTCAGGATAGGTT209310.3277817719759505No Hit
TTCTCACTACTGCACTTGACTAG196820.30822229401512863No Hit
CACCCGGCTGTGTGCACATGTGC195100.3055287550165206No Hit
ACCGGGTGCTGTAGGCTT179380.2809110613780803No Hit
CAACGGAATCCCAAAAGCAGCT171650.2688057959948015No Hit
TCGTACGACTCTTAGCGG162560.2545707555893675No Hit
CATTGCACTTGTCTCGGTCTGA159690.25007630388820185No Hit
CTTCTCACTACTGCACTTGACTAG157870.24722616378502366No Hit
TATTGCACTTGTCCCGGCCTGTAG157600.24680334080268404No Hit
CCCATAAAGTAGAAAGCACTAC149860.23468241530894815No Hit
CTCCCACTGCTTCACTTGACTAG142140.2225928100361263No Hit
ACCCCCCACTGCTAAATTTGACTG141370.22138698153093556No Hit
CAACACCAGTCGATGGGCTGT129430.20268880964524993No Hit
CACTAGATTGTGAGCTCCTGGA128380.20104449804726252No Hit
CTACGCCTGTCTGAGCGTCGCT127630.1998699897629858No Hit
CGCGACCTCAGATCAGAC127540.1997290487688726No Hit
CCCATAAAGTAGAAAGCACT122800.19230615641224363No Hit
CCCCCCACTGCTAAATTTGACTGG122470.19178937276716188No Hit
TACGCCTGTCTGAGCGTCGCT122290.19150749077893547No Hit
TGAGGTAGTAGTTTGTACAGTT121480.19023902183191657No Hit
ACCGGGTGCTGTAGGCTTA117990.18477364328241552No Hit
GGAATACCGGGTGCTGTAGGCTTT117900.1846327022883023No Hit
TATTGCACTTGTCCCGGCCTGTT114540.17937090517474258No Hit
CCCCCACTGCTAAATTTGACTGGCTT114110.17869752042509054No Hit
TAGCTTATCAGACTGATGTTGACA111440.1745162709330654No Hit
CACTAGATTGTGAGCTCCTGGAA109190.1709927460802352No Hit
ACTGGACTTGGAGTCAGAAGGCT106720.1671246987973505No Hit
ACCGGGTGCTGTAGGCTTT106220.16634169327449935No Hit
AACGGAATCCCAAAAGCAGCTG104910.1642902188046293No Hit
TCGTTTCCCGGCCAATGCACCA103350.16184724157333372No Hit
ACTGGACTTGGAGTCAGAAGGCA102130.1599367080975769No Hit
ACGCCTGTCTGAGCGTCGCT100840.1579165538486209No Hit
TGATGTAGTAGGTTGTATAGTT100400.1572275089885119No Hit
TATTGCACTTGTCCCGGCCTGTAAT99810.15630356247154756No Hit
AGCTCGGTCTGAGGCCCCTCAGT97360.1524668354095769No Hit
CAAAGAATTCTCCTTTTGGGCT93990.1471893781855601No Hit
AACATTCAACGCTGTCGGTGAGA93320.14614015078493955No Hit
GGAATACCGGGTGCTGTAGGCTT92080.14419829708826867No Hit
TTCAAGTAATCCAGGATAGGCTT91360.143070769135363No Hit
TGTAAACATCCTACACTCTCAGCT88850.1391400814106502No Hit
TGAGGTAGTAGTTTGTGCTGTT87470.13697898616758103No Hit
CCCCCCACTGCTAAATTTGACTGT86060.13477091059314078No Hit
CTCCCACTGCTTCACTTGACTAGCCT84260.13195209071087663No Hit
TGTCAGTTTGTCAAATACCCCAA83770.13118474529848248No Hit
TAGCAGCACGTAAATATTGGC83470.1307149419847718No Hit
TGTAAACATCCCCGACTGGAAGC81980.12838158552667533No Hit
CCCCCCACTGCTAAATTTGACTGGCT81860.12819366420119108No Hit
CAAAGAATTCTCCTTTTGGGCTT81060.12694085536462923No Hit
TGAGGTAGTAGGTTGTGTGGTT79810.12498334155750133No Hit
TATTGCACTTGTCCCGGCCTGTAAA79620.1246857994588179No Hit
GCGTTCGCGCTTTCCCCTG79430.12438825736013445No Hit
CGCGACCTCAGATCAGACGTGGCG78390.12275960587260407No Hit
AACATTCAACGCTGTCGGTGA76470.11975286466485563No Hit
ATCCCGGACGAGCCCCCA74270.11630764036431053No Hit
AGCAGCATTGTACAGGGCTATGT73730.1154619943996313No Hit
ACTTGACTTGGAGTCAGAAGGC72940.11422484567352646No Hit
TTAATGCTAATCGTGATAGGGGTTT70500.11040377872201285No Hit
ACCACAGGGTAGAACCACGGAC70240.10999661585013024No Hit
TATTGCACTTGTCCCGGCCTGTTT70150.10985567485601704No Hit
GACCCGCGGGCGCTCTCCAGTCCT70010.1096364333096187No Hit
CCCCCCACTGCTAAATTTGACTGTA67700.10601894779404637No Hit
TTCAAGTAATCCAGGATAGGC65850.10312182735949708No Hit
CACCCGTAGAACCGACCTTGCG65700.10288692570264174No Hit
TGAGAACTGAATTCCATGGGTT65050.10186901852293526No Hit
ACTGGACTTGGAGTCAGAAGG64280.10066319001774447No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)