FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947244

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947244
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8764597
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG4393595.012883079507249No Hit
AACATTCAACGCTGTCGGTGAGT4334614.945589626083207No Hit
AAGCTGCCAGTTGAAGAACTGT3827504.367000559181443No Hit
TTCAAGTAATCCAGGATAGGCT3171433.6184550185250957No Hit
TAGCAGCACGTAAATATTGGCG2984443.405108072852637No Hit
CCCCCCACTGCTAAATTTGACTG2382832.7186988745746095No Hit
AACATTCAACGCTGTCGGTGAG2004312.2868250531085454No Hit
TAGCTTATCAGACTGATGTTGAC1567951.7889584655175816No Hit
TAGCTTATCAGACTGATGTTGA1364071.5563408106499363No Hit
TAGCTTATCAGACTGATGTTGACT1359211.550795775322014No Hit
CGCCTGTCTGAGCGTCGCT1303491.4872218311920105No Hit
TGAGGTAGTAGGTTGTATAGTT1265521.443899816500405No Hit
TATTGCACTTGTCCCGGCCTGT927911.058702413813208No Hit
TATTGCACTTGTCCCGGCCTGTAA919081.0486277920137115No Hit
TATTGCACTTGTCCCGGCCTGTAT883381.0078957423826789No Hit
CCCCCACTGCTAAATTTGACTG835580.9533581521203998No Hit
CATAAAGTAGAAAGCACTACT823250.9392901921217827No Hit
TGAGGTAGTAGATTGTATAGTT819120.9345780530468201No Hit
TGTAAACATCCTTGACTGGAAGCT798360.9108918527571775No Hit
TTCAAGTAATTCAGGATAGGTT640110.7303359184683562No Hit
AGCAGCATTGTACAGGGCTATGA582210.6642746951171856No Hit
TCGTACGACTCTTAGCGG562560.64185495351355No Hit
ACTGGACTTGGAGTCAGAAGGC500280.5707963526446225No Hit
TATTGCACTTGTCCCGGCCTGTA478510.5459577890460907No Hit
TGTAAACATCCCCGACTGGAAGCT466270.5319925148868796No Hit
TGAGGTAGTAGTTTGTACAGTT394080.44962706214558407No Hit
CCCATAAAGTAGAAAGCACT361130.4120326353852893No Hit
AACATTCAACGCTGTCGGTGAGTT338490.38620144200583323No Hit
CATTGCACTTGTCTCGGTCTGA327770.37397041757881166No Hit
TACGCCTGTCTGAGCGTCGCT326940.37302342594873444No Hit
CCCATAAAGTAGAAAGCACTAC324520.37026231782248514No Hit
ACGCCTGTCTGAGCGTCGCT300200.34251432210745114No Hit
CAACGGAATCCCAAAAGCAGCTGT299310.3414988732511033No Hit
CAACACCAGTCGATGGGCTGT292310.3335121968528616No Hit
TGATGTAGTAGGTTGTATAGTT273780.31237032347294463No Hit
CTACGCCTGTCTGAGCGTCGCT260380.29708154293916766No Hit
TGTAAACATCCTACACTCTCAGCT256840.2930425665891997No Hit
TGTCAGTTTGTCAAATACCCCAA252140.287680083864666No Hit
TTATAAAGCAATGAGACTGATT217940.24865946489039942No Hit
GCGTTCGCGCTTTCCCCTG216160.24662856717770368No Hit
TTCAAGTAATCCAGGATAGGCTT211660.24149427520740543No Hit
TTCTCACTACTGCACTTGACTAG208460.2378432231396378No Hit
GCCTGTCTGAGCGTCGCT196810.22455111170542127No Hit
TGAGGTAGTAGTTTGTGCTGTT193460.22072891657197702No Hit
CACCCGGCTGTGTGCACATGTGC184110.21006099881146845No Hit
ACCGGGTGCTGTAGGCTT181960.2076079482034371No Hit
CTTCTCACTACTGCACTTGACTAG174970.19963268134290715No Hit
TGATGTAGTAGATTGTATAGTT174300.1988682423162183No Hit
CAACGGAATCCCAAAAGCAGCT172450.19675747783954012No Hit
TTCACAGTGGCTAAGTTCCG164860.18809763871630378No Hit
ATCCCGGACGAGCCCCCA157440.1796317617341676No Hit
TAGCTTATCAGACTGATGTTGACA152270.1737330307371805No Hit
CGCGACCTCAGATCAGACGTGGCG149680.17077796046983107No Hit
AACATTCAACGCTGTCGGTGA149470.17053836017788382No Hit
TATTGCACTTGTCCCGGCCTGTT148190.16907793935077678No Hit
ATCCCACCGCTGCCACCA147100.16783429974019343No Hit
TTCAAGTAATCCAGGATAGGC145950.1665222029033394No Hit
TAGCAGCACGTAAATATTGGC145180.16564366849953283No Hit
TGTCAGTTTGTCAAATACCCCA143630.1638751901542079No Hit
ACCACAGGGTAGAACCACGGAC143150.16332753234404274No Hit
CGCGACCTCAGATCAGACGTGG140200.15996171871906945No Hit
CTCCCACTGCTTCACTTGACTAG139360.15900331755128047No Hit
AGCAGCATTGTACAGGGCTATGT136340.15555763716232476No Hit
TGTAAACATCCCCGACTGGAAGC135420.15450795969284156No Hit
TGTAAACATCCTACACTCAGCT132940.15167839434032163No Hit
CAAAGTGCTGTTCGTGCAGGT130710.14913406743059607No Hit
TTAGGTAGTAGGTTGTATAGTT130250.14860922869585447No Hit
CAAAGAATTCTCCTTTTGGGCTT129110.14730854139671226No Hit
TGAGGTAGTAGGTTGTATAGT128260.14633873069121148No Hit
ACTGGACTTGGAGTCAGAAGG127710.1457112061170639No Hit
TCGTTCGCGCTTTCCCCTG122720.14001784679888876No Hit
TAGCAGCACGTAAATATTGGCGT121250.138340644755258No Hit
TGAGGTAGTAGGTTGTGTGGTT120010.13692586207899804No Hit
AACATTCAACGCTGTCGGTGAGA119600.13645807103281532No Hit
ACCGGGTGCTGTAGGCTTT118430.1351231551205378No Hit
AACGGAATCCCAAAAGCAGCTG118130.13478086898918454No Hit
CAAAGAATTCTCCTTTTGGGCT118030.13466677361206683No Hit
TTCACAGTGGCTAAGTTCCGC117200.13371978198198958No Hit
CCTCGACACAAGGGTTTGT116780.13324058139809508No Hit
AGCTCGGTCTGAGGCCCCTCAGT116680.13312648602097735No Hit
ACTGGACTTGGAGTCAGAAGGCT115150.13138082675107596No Hit
ACTTGACTTGGAGTCAGAAGGC114480.1306163877243871No Hit
ACTGGACTTGGAGTCAGAAGGCA113830.1298747677731218No Hit
TGCAAGTAATCCAGGATAGGCT113800.12984053915998647No Hit
AACCGGGCGGAAACACCA113700.12972644378286874No Hit
TTCACAGTGGCTAAGTTCTGC110790.12640626830874255No Hit
AGCAGCATTGTACAGGGCTATG110230.1257673341968832No Hit
CGCGACCTCAGATCAGAC106660.12169412923377994No Hit
TCCTGTCTGAGCGTCGCT105370.12022229886896113No Hit
ACCGGGTGCTGTAGGCTTA105270.12010820349184338No Hit
TTCACAGTGGCTAAGTTCTG102550.11700480923424088No Hit
TATTGCACTTGTCCCGGCCTGTAG100490.11465444446561547No Hit
CCCCCCACTGCTAAATTTGACTGGCT98980.11293160427113763No Hit
TAGCTTATCAGACTGATGTTG98560.11245240368724312No Hit
AGAGGTAGTAGGTTGCATAGTT97030.11070674441734173No Hit
CACTAGATTGTGAGCTCCTGGA95870.10938323804277594No Hit
CAAAGTGCTGTTCGTGCAGGTAG95000.10839060826185164No Hit
CCAAGTCCTTCTGATCGAGGCCC94610.10794563629109245No Hit
CCCCCCACTGCTAAATTTGACTGG93850.10707851142499764No Hit
TTTAAACATCCTTGACTGGAAGCT92660.10572077643729655No Hit
CCCCCACTGCTAAATTTGACTGGCTT91290.10415766977078353No Hit
CTCCTGTCTGAGCGTCGCT90980.10380397410171854No Hit
TGAGGTAGTAAGTTGTATTGTT87940.10033547463733929No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)