FastQCFastQC Report
Sun 4 Sep 2022
EGAF00003947245

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947245
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4570849
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG2505935.482416942673013No Hit
AAGCTGCCAGTTGAAGAACTGT2046534.477352019285695No Hit
GCCTGTCTGAGCGTCGCT1568583.431703825700652No Hit
TAGCAGCACGTAAATATTGGCG1491053.2620854462704854No Hit
TTCAAGTAATCCAGGATAGGCT1455913.1852069495185686No Hit
AACATTCAACGCTGTCGGTGAGT1426783.1214769947552417No Hit
TAGCTTATCAGACTGATGTTGA835391.8276473364138697No Hit
CGCCTGTCTGAGCGTCGCT779721.7058537702733123No Hit
AACATTCAACGCTGTCGGTGAG740641.620355430686947No Hit
CCCCCCACTGCTAAATTTGACTG735591.6093071549727413No Hit
CATAAAGTAGAAAGCACTACT677831.4829411341306615No Hit
TAGCTTATCAGACTGATGTTGAC642321.4052531597521598No Hit
TGAGGTAGTAGGTTGTATAGTT580221.2693921851279706No Hit
TATTGCACTTGTCCCGGCCTGT560831.2269711819401603No Hit
TCCTGTCTGAGCGTCGCT524801.1481455633297009No Hit
TAGCTTATCAGACTGATGTTGACT408310.893291377597466No Hit
TGAGGTAGTAGATTGTATAGTT343460.7514140152081156No Hit
TTCAAGTAATTCAGGATAGGTT337890.7392280952619524No Hit
TATTGCACTTGTCCCGGCCTGTAA329480.7208288875874045No Hit
TATTGCACTTGTCCCGGCCTGTAT316820.6931316260939707No Hit
AGCAGCATTGTACAGGGCTATGA301790.6602493322356525No Hit
CCCCCACTGCTAAATTTGACTG271950.594966055540229No Hit
TGTAAACATCCTTGACTGGAAGCT247510.5414967766382132No Hit
TCGTACGACTCTTAGCGG219440.48008586588618435No Hit
CATTGCACTTGTCTCGGTCTGA217150.4750758557108319No Hit
TATTGCACTTGTCCCGGCCTGTA212840.4656465352497971No Hit
ATCCCGGACGAGCCCCCA206060.4508134046869629No Hit
CCCATAAAGTAGAAAGCACT196110.4290450198639246No Hit
CCCATAAAGTAGAAAGCACTAC195810.42838868665317975No Hit
ACTGGACTTGGAGTCAGAAGGC190560.41690285546514444No Hit
ACGCCTGTCTGAGCGTCGCT181110.3962283593266809No Hit
TGATGTAGTAGGTTGTATAGTT159630.34923490143734787No Hit
ACCGGGTGCTGTAGGCTT155960.3412057584925689No Hit
CAACGGAATCCCAAAAGCAGCTGT153740.33634889273305685No Hit
CTACGCCTGTCTGAGCGTCGCT148700.3253224947925429No Hit
TACGCCTGTCTGAGCGTCGCT143590.31414295243618856No Hit
TGAGGTAGTAGTTTGTACAGTT140520.30742647591289934No Hit
TGTAAACATCCCCGACTGGAAGCT139020.30414480985917497No Hit
TTAATGCTAATCGTGATAGGGGTT137460.30073187716330163No Hit
ATCCCACCGCTGCCACCA127310.27852593686643334No Hit
GCGTTCGCGCTTTCCCCTG117700.25750139634890584No Hit
TTCACAGTGGCTAAGTTCCG113080.24739386490343476No Hit
CAACACCAGTCGATGGGCTGT112500.24612495402932802No Hit
TGAGGTAGTAGTTTGTGCTGTT108280.2368925335315168No Hit
TGTAAACATCCTACACTCTCAGCT108000.2362799558681549No Hit
TTATAAAGCAATGAGACTGATT100570.22002477001537352No Hit
AACATTCAACGCTGTCGGTGAGTT100290.21941219235201162No Hit
ACCGGGTGCTGTAGGCTTT96410.21092361615971125No Hit
TGATGTAGTAGATTGTATAGTT92770.2029601065360068No Hit
CACCCGGCTGTGTGCACATGTGC91980.20123176241437862No Hit
ACGCGACCTCAGATCAGA88060.19265567512731224No Hit
TACCTGGTTGATCCTGCC86610.18948339794204536No Hit
AGCTCGGTCTGAGGCCCCTCAGT84540.1849546987879057No Hit
TTCAAGTAATCCAGGATAGGC84120.18403583229286288No Hit
CTCCCACTGCTTCACTTGACTAG84030.1838389323296394No Hit
CGCGACCTCAGATCAGACGTGG83660.18302945470305407No Hit
TTCTCACTACTGCACTTGACTAG82840.1812354772603514No Hit
TAGCAGCACGTAAATATTGGC81130.1774943779591056No Hit
TTCAAGTAATCCAGGATAGGCTT80140.17532847836364754No Hit
TATTGCACTTGTCCCGGCCTGTT74780.16360199166500577No Hit
TGTAAACATCCTACACTCAGCT74290.16252998075412248No Hit
AACCGGGCGGAAACACCA72430.15846071484750426No Hit
TTCACAGTGGCTAAGTTCCGC71550.15653547076265262No Hit
CAAAGTGCTGTTCGTGCAGGT69800.15270686036664086No Hit
TAGCAGCACGTAAATATTGGCGT69320.15165672722944906No Hit
TGAGGTAGTAGGTTGTATAGT68810.15054096077118279No Hit
TGAGGTAGTAGGTTGTGTGGTT68250.149315805444459No Hit
AGCAGCATTGTACAGGGCTATG67370.14739056135960737No Hit
ACCGGGTGCTGTAGGCTTA66370.1452027839904578No Hit
ACCACAGGGTAGAACCACGGAC66230.14489649515877684No Hit
TAGCTTATCAGACTGATGTTG65000.14220552899472286No Hit
AGCAGCATTGTACAGGGCTATGT64340.14076159593108414No Hit
CCTCGACACAAGGGTTTGT61350.1342201415973269No Hit
TAGCAGCACATAATGGTTTGT61010.13347629729181604No Hit
TAGCTTATCAGACTGATGTTGACA60820.13306061959167761No Hit
CTTCTCACTACTGCACTTGACTAG58520.12802873164263356No Hit
ACTTGACTTGGAGTCAGAAGGC57870.12660667635268635No Hit
TGAGAACTGAATTCCATGGGTT56510.12363129913064291No Hit
TTCACAGTGGCTAAGTTCTGC55170.12069967745598247No Hit
TGTCAGTTTGTCAAATACCCCAA54860.12002146647154609No Hit
TTCACCACCTTCTCCACCCAGC54780.11984644428201412No Hit
AACGGAATCCCAAAAGCAGCTG54670.11960578877140766No Hit
TTCACAGTGGCTAAGTTCTG53970.11807434461300298No Hit
CAAAGTGCTGTTCGTGCAGGTAG53770.11763678913917305No Hit
CACTAGATTGTGAGCTCCTGGA53150.11628036717030031No Hit
CGCGACCTCAGATCAGACGTGGCG51370.11238612345321405No Hit
AATGACACGATCACTCCCGTTG50210.10984830170500054No Hit
TCCCCGGCACCTCCACCA49380.10803244648860638No Hit
TGTCAGTTTGTCAAATACCCCA49280.10781366875169142No Hit
TGTAAACATCCCCGACTGGAAGC49140.1075073799200105No Hit
AACATTCAACGCTGTCGGTGA47980.10496955817179696No Hit
CCCATAAAGTAGAAAGCACTA47610.10416008054521161No Hit
GTCCGGCCGTGTCGGCGG47280.10343811401339227No Hit
TATTGCACTTGTCCCGGCCTGA47030.10289116967110487No Hit
CTCGACACAAGGGTTTGT46300.10129409219162566No Hit
CAACGGAATCCCAAAAGCAGCT46140.10094404781256174No Hit
CATAAAGTAGAAAGCACTAC45770.1001345701859764No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)