FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947247

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947247
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6580441
Sequences flagged as poor quality0
Sequence length18-26
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG4409966.701617718326173No Hit
CCCCCCACTGCTAAATTTGACTG4022656.1130401442699664No Hit
AACATTCAACGCTGTCGGTGAGT2481133.770461584565533No Hit
AACATTCAACGCTGTCGGTGAG1925102.9254878206491024No Hit
TTCAAGTAATCCAGGATAGGCT1883732.8626196937256942No Hit
TAGCTTATCAGACTGATGTTGAC1668972.5362585881402175No Hit
TAGCAGCACGTAAATATTGGCG1663512.527961271896519No Hit
TAGCTTATCAGACTGATGTTGACT1624292.4683604032009403No Hit
CCCCCACTGCTAAATTTGACTG1542472.344022231944637No Hit
TAGCTTATCAGACTGATGTTGA1369392.081000346329372No Hit
AAGCTGCCAGTTGAAGAACTGT1259931.914658911158082No Hit
TATTGCACTTGTCCCGGCCTGTAA1206461.8334029588594443No Hit
TGAGGTAGTAGGTTGTATAGTT1107281.6826835769821507No Hit
TATTGCACTTGTCCCGGCCTGTAT1042341.583997181951787No Hit
TGAGGTAGTAGATTGTATAGTT808381.2284587005642935No Hit
TGTAAACATCCTTGACTGGAAGCT539070.8192004152913155No Hit
TTCAAGTAATTCAGGATAGGTT506320.769431714379021No Hit
TATTGCACTTGTCCCGGCCTGTA491170.7464089412852422No Hit
TATTGCACTTGTCCCGGCCTGT477840.726151940272696No Hit
CATAAAGTAGAAAGCACTACT476130.7235533302403289No Hit
AGCAGCATTGTACAGGGCTATGA436200.6628735065020718No Hit
TGTAAACATCCCCGACTGGAAGCT426530.6481784427517852No Hit
ACTGGACTTGGAGTCAGAAGGC337410.5127467900707566No Hit
CAACGGAATCCCAAAAGCAGCT319890.4861224346514162No Hit
CATTGCACTTGTCTCGGTCTGA310180.471366584701542No Hit
CCCCCACTGCTAAATTTGACTGGCTT290400.44130780900550587No Hit
TGAGGTAGTAGTTTGTACAGTT272620.41428834328884645No Hit
CAACACCAGTCGATGGGCTGT261640.397602531502068No Hit
TGATGTAGTAGGTTGTATAGTT248150.37710238569117177No Hit
TAGCTTATCAGACTGATGTTGACA244140.37100856918252134No Hit
TCGTACGACTCTTAGCGG237590.36105482900006247No Hit
GCCTGTCTGAGCGTCGCT230500.35028047512317184No Hit
CAACGGAATCCCAAAAGCAGCTGT226930.34485530680998433No Hit
CAAAGAATTCTCCTTTTGGGCT218870.3326068875930959No Hit
CCCATAAAGTAGAAAGCACT207840.3158450930568331No Hit
CACCCGGCTGTGTGCACATGTGC199470.3031255807931414No Hit
TTCTCACTACTGCACTTGACTAG190050.2888104307902768No Hit
CCCATAAAGTAGAAAGCACTAC187990.28567994151151876No Hit
GGAATACCGGGTGCTGTAGGCTTT183080.2782184355121488No Hit
TGATGTAGTAGATTGTATAGTT181720.2761517047261726No Hit
AACATTCAACGCTGTCGGTGAGTT173430.26355376486165594No Hit
TTATAAAGCAATGAGACTGATT168330.25580352441424514No Hit
CACTAGATTGTGAGCTCCTGGAA166500.25302255578311544No Hit
CTTCTCACTACTGCACTTGACTAG162780.24736943922147467No Hit
TGTAAACATCCTACACTCTCAGCT161880.24600174973075511No Hit
CACTAGATTGTGAGCTCCTGGA161690.24571301528271433No Hit
TGAGGTAGTAGTTTGTGCTGTT151290.22990860338995517No Hit
TATTGCACTTGTCCCGGCCTGTAG147320.22387557308089232No Hit
TAGCAGCACGTAAATATTGGC131390.19966746909515637No Hit
TCGTTTCCCGGCCAATGCACCA127340.19351286638691845No Hit
ACTGGACTTGGAGTCAGAAGGCT127290.19343688363743403No Hit
CAAAGAATTCTCCTTTTGGGCTT122820.18664402583352696No Hit
CCCCCCACTGCTAAATTTGACTGGCT120610.18328558830631564No Hit
GGAATACCGGGTGCTGTAGGCTT113730.17283036197725957No Hit
TTCACAGTGGCTAAGTTCTG111010.1686969004053072No Hit
TTCAAGTAATCCAGGATAGGCTT110900.16852973835644147No Hit
AGCAGCATTGTACAGGGCTATGT109430.16629584552159954No Hit
TATTGCACTTGTCCCGGCCTGTAAT108750.16526248012861144No Hit
ACTGGACTTGGAGTCAGAAGGCA107830.16386439753809812No Hit
TCCTGTCTGAGCGTCGCT106660.1620864012001627No Hit
TATTGCACTTGTCCCGGCCTGTT102300.1554607054451214No Hit
TGTAAACATCCCCGACTGGAAGC102110.15517197099708058No Hit
TATTGCACTTGTCCCGGCCTGTAAA101120.1536675125572891No Hit
AACGGAATCCCAAAAGCAGCTG100950.15340917120904207No Hit
ATGCACCTGGGCAAGGATTCTGA98770.1500963233315214No Hit
ACCGGGTGCTGTAGGCTT98420.14956444408513048No Hit
ACCCCCCACTGCTAAATTTGACTG96110.1460540410589503No Hit
TTCACAGTGGCTAAGTTCCG96080.14600845140925967No Hit
CAACGGAATCCCAAAAGCAGCTGA94180.14312110692885174No Hit
TGAGGTAGTAAGTTGTATTGTT92370.14037053139751576No Hit
TGAATACCGGGTGCTGTAGGCTTT90670.1377871179150455No Hit
CTCCCACTGCTTCACTTGACTAG88660.13473261138577186No Hit
TGAGGTAGTAGGTTGTATAGT87720.1333041356954648No Hit
AACATTCAACGCTGTCGGTGA87000.13220998410288914No Hit
TTCAAGTAATCCAGGATAGGC86480.1314197635082512No Hit
AGAATTACTCTGAGACCT84990.1291554775736155No Hit
ACTTGACTTGGAGTCAGAAGGC83900.12749905363485517No Hit
CTCCCACTGCTTCACTTGACTAGCCT83460.12683040543939228No Hit
CCCATAAAGTAGAAAGCACTA82880.12594900554537303No Hit
AATGCACCCGGGCAAGGATTCT82040.12467249535403478No Hit
TGTCAGTTTGTCAAATACCCCA80120.1217547577738331No Hit
ACTGGACTTGGAGTCAGAAGG79900.12142043367610164No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC79330.12055423033197926No Hit
CCCCCCACTGCTAAATTTGACTGG76590.11639037566023311No Hit
CGCCTGTCTGAGCGTCGCT76390.11608644466229542No Hit
TTCACAGTGGCTAAGTTCTGC74730.11356381737941271No Hit
TGTCAGTTTGTCAAATACCCCAA73260.11132992454457079No Hit
AGCAGCATTGTACAGGGCTATG73040.11099560044683936No Hit
TTCACAGTGGCTAAGTTCCGC70840.10765235946952492No Hit
AGCTCGGTCTGAGGCCCCTCAGT70470.10709008712334021No Hit
TGAGGTAGTAGATTGTATAGT70170.1066341906264337No Hit
TATTGCACTTGTCCCGGCCTGTTT69570.10572239763262066No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)