FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947248

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947248
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7929470
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG79344210.006242535755858No Hit
CAACGGAATCCCAAAAGCAGCTG4657235.87331814106113No Hit
CCCCCACTGCTAAATTTGACTG2512503.1685598154731656No Hit
AAGCTGCCAGTTGAAGAACTGT2271032.864037571237422No Hit
TAGCAGCACGTAAATATTGGCG2264702.8560546921799315No Hit
AACATTCAACGCTGTCGGTGAGT2106122.656066546692276No Hit
TTCAAGTAATCCAGGATAGGCT1629152.0545509346778537No Hit
TAGCTTATCAGACTGATGTTGAC1441291.8176372443555497No Hit
AACATTCAACGCTGTCGGTGAG1046601.31988644890516No Hit
TAGCTTATCAGACTGATGTTGACT1042871.315182477517413No Hit
TATTGCACTTGTCCCGGCCTGTAA982441.2389730965625698No Hit
TATTGCACTTGTCCCGGCCTGTAT895681.1295584698599024No Hit
TAGCTTATCAGACTGATGTTGA846971.0681293957855946No Hit
TATTGCACTTGTCCCGGCCTGT789270.9953628678839822No Hit
AGCAGCATTGTACAGGGCTATGA592450.7471495572844087No Hit
TGTAAACATCCTTGACTGGAAGCT589980.7440345949981525No Hit
TATTGCACTTGTCCCGGCCTGTA488100.6155518590775929No Hit
CATAAAGTAGAAAGCACTACT410640.5178656328859306No Hit
TGTAAACATCCCCGACTGGAAGCT401340.5061372323749255No Hit
TGAGGTAGTAGGTTGTATAGTT401260.5060363429081641No Hit
ACTGGACTTGGAGTCAGAAGGC384040.48431988518778685No Hit
TTCAAGTAATTCAGGATAGGTT329350.4153493234730694No Hit
CGCGACCTCAGATCAGACGTGGCG293890.37063006733110787No Hit
CAACGGAATCCCAAAAGCAGCTGT292590.3689906134962362No Hit
CCCATAAAGTAGAAAGCACTAC255210.3218500101520026No Hit
ACCGGGTGCTGTAGGCTT245260.30930188272356157No Hit
GCCTGTCTGAGCGTCGCT231780.2923020075742767No Hit
CGCCTGTCTGAGCGTCGCT226900.2861477501018353No Hit
CCCCCACTGCTAAATTTGACTGGCTT226290.28537846791778015No Hit
CATTGCACTTGTCTCGGTCTGA223580.28196083723123994No Hit
TCGTACGACTCTTAGCGG221010.27871976311153207No Hit
TAGCTTATCAGACTGATGTTGACA218980.27615969289246317No Hit
ACCCCCCACTGCTAAATTTGACTG212660.2681894250183177No Hit
TGAGGTAGTAGATTGTATAGTT209250.26388901149761584No Hit
TGTAAACATCCTACACTCTCAGCT204520.25792392177535195No Hit
CGCGACCTCAGATCAGACGTGG201260.25381267600482754No Hit
CAACGGAATCCCAAAAGCAGCT190050.23967553947489553No Hit
AACATTCAACGCTGTCGGTGAGTT189100.2384774770571047No Hit
CCCCCCACTGCTAAATTTGACTGG183400.23128910255035962No Hit
GGGCTACGCCTGTCTGAGCGTCGCT179600.22649685287919621No Hit
CTCCCACTGCTTCACTTGACTAG170530.2150585095851299No Hit
ACTTGACTTGGAGTCAGAAGGC157440.19855047058630654No Hit
ACCGGGTGCTGTAGGCTTT155980.1967092378179122No Hit
CCCATAAAGTAGAAAGCACT155830.19652007006773467No Hit
TGATGTAGTAGGTTGTATAGTT150250.18948302976113157No Hit
TATTGCACTTGTCCCGGCCTGTT141410.1783347436840041No Hit
ACCGGGTGCTGTAGGCTTA141000.17781768516685226No Hit
TCCTGTCTGAGCGTCGCT140460.17713668126621326No Hit
CAACACCAGTCGATGGGCTGT135920.17141120402750754No Hit
CTCCCCACTGCTAAATTTGACTG134500.16962041599249383No Hit
CTTCTCACTACTGCACTTGACTAG134200.16924208049213882No Hit
CCCCCCACTGCTAAATTTGACTGTA133950.16892680090850964No Hit
CCCCCCACTGCTAAATTTGACTGT132840.16752695955719613No Hit
GTGCGGTAACGCGACCGATCCCGGA132300.1668459556565571No Hit
CACTAGATTGTGAGCTCCTGGA132110.16660634317299897No Hit
TTCAAGTAATCCAGGATAGGCTT132100.16659373198965377No Hit
CGCGACCTCAGATCAGACGTGGC128640.16223026255222606No Hit
CAAAGAATTCTCCTTTTGGGCT127140.16033858505045104No Hit
CCCCCCACTGCTAAATTTGACTGGCT126030.15893874369913752No Hit
AGCAGCATTGTACAGGGCTATGT124020.15640389584675898No Hit
CGCCCCACTGCTAAATTTGACTG123110.1552562781623488No Hit
TGAAAAGAACTTTGAAGAGAG118920.1499721923407239No Hit
CAAAGAATTCTCCTTTTGGGCTT117720.1484588503393039No Hit
CTACGCCTGTCTGAGCGTCGCT117080.14765173460521322No Hit
TTCTCACTACTGCACTTGACTAG116750.1472355655548227No Hit
CACTAGATTGTGAGCTCCTGGAA112510.141888423816472No Hit
AGCTCGGTCTGAGGCCCCTCAGT112500.14187581263312682No Hit
TATTGCACTTGTCCCGGCCTGTAG111850.14105608571569095No Hit
TGGCTACGCCTGTCTGAGCGTCGCT111650.14080386204878761No Hit
TAGCAGCACGTAAATATTGGC110670.13956796608096128No Hit
CCCGCCACTGCTAAATTTGACTG104330.13157247584012552No Hit
TGTAAACATCCCCGACTGGAAGC102900.12976907662176665No Hit
TACGCCTGTCTGAGCGTCGCT100530.12678022616896212No Hit
ACCACAGGGTAGAACCACGGAC99180.1250777164173646No Hit
CTCCCACTGCTTCACTTGACTAGCCT95120.11995757597922686No Hit
AGCAGCATTGTACAGGGCTATG94050.118608179361294No Hit
ACTGGACTTGGAGTCAGAAGGCA90590.11424470992386629No Hit
TTATAAAGCAATGAGACTGATT89550.11293314685596895No Hit
TTGCGGTAACGCGACCGATCCCGGA88100.11110452527091975No Hit
TAGCAGCACGTAAATATTGGCGT86870.10955334971946423No Hit
TGCAAGTAATCCAGGATAGGCT86440.10901106883562206No Hit
TATTGCACTTGTCCCGGCCTGTAAT85680.10805261890138938No Hit
CGCTACCTCAGATCAGACGTGGCG84100.10606005193285302No Hit
TGAGGTAGTAGTTTGTACAGTT82020.10343692579705831No Hit
ACCTGGTGCTGTAGGCTT79640.1004354641609086No Hit
CAAAGTGCTGTTCGTGCAGGTAG79610.10039763061087312No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)