FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003947249

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947249
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16286934
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG16170999.928811647422405No Hit
CAACGGAATCCCAAAAGCAGCTG11478787.047845837651212No Hit
CCCCCACTGCTAAATTTGACTG4930093.027021537632559No Hit
AAGCTGCCAGTTGAAGAACTGT4705452.8890950254971255No Hit
AACATTCAACGCTGTCGGTGAGT4087712.509809396906747No Hit
TTCAAGTAATCCAGGATAGGCT4068772.4981804432927643No Hit
TAGCAGCACGTAAATATTGGCG3703212.2737305867390387No Hit
TAGCTTATCAGACTGATGTTGAC2632471.6163078944140132No Hit
AACATTCAACGCTGTCGGTGAG2230131.3692755186458052No Hit
TAGCTTATCAGACTGATGTTGACT1932421.18648482274196No Hit
TATTGCACTTGTCCCGGCCTGTAA1781071.0935575719776356No Hit
TATTGCACTTGTCCCGGCCTGTAT1532130.9407111246352444No Hit
TGTAAACATCCTTGACTGGAAGCT1400080.859633863562043No Hit
TAGCTTATCAGACTGATGTTGA1340300.8229295949747202No Hit
TGAGGTAGTAGGTTGTATAGTT1338290.8216954768773546No Hit
TATTGCACTTGTCCCGGCCTGT1181930.7256921407061636No Hit
AGCAGCATTGTACAGGGCTATGA1079360.6627152783943251No Hit
TGAGGTAGTAGATTGTATAGTT883320.5423488546094679No Hit
TGTAAACATCCCCGACTGGAAGCT813320.4993696173877784No Hit
TATTGCACTTGTCCCGGCCTGTA809330.4969198008661422No Hit
TTAATGCTAATCGTGATAGGGGTT800480.49148599730311426No Hit
TTCAAGTAATTCAGGATAGGTT774430.4754915811656141No Hit
CCCCCACTGCTAAATTTGACTGGCTT750010.4604979672662762No Hit
ACTGGACTTGGAGTCAGAAGGC733150.4501461109868807No Hit
CATAAAGTAGAAAGCACTACT694080.42615755672614625No Hit
CAACGGAATCCCAAAAGCAGCTGT691840.4247822211350522No Hit
ACCCCCCACTGCTAAATTTGACTG634540.38960064552358353No Hit
CGCGACCTCAGATCAGACGTGGCG592430.36374556438922145No Hit
CCCATAAAGTAGAAAGCACTAC589330.3618421981694038No Hit
ACCGGGTGCTGTAGGCTT538360.3305471735809821No Hit
TAGCTTATCAGACTGATGTTGACA476330.2924614295115336No Hit
CGCCTGTCTGAGCGTCGCT465970.28610050240272356No Hit
GGGCTACGCCTGTCTGAGCGTCGCT450430.2765591117395085No Hit
CTCCCACTGCTTCACTTGACTAG432450.26551958766456596No Hit
CCCCCCACTGCTAAATTTGACTGG415540.25513703193001214No Hit
ACCGGGTGCTGTAGGCTTA408120.25058123278451305No Hit
CCCCCCACTGCTAAATTTGACTGGCT393020.24130999732669142No Hit
TTCTCACTACTGCACTTGACTAG376730.23130811483610111No Hit
ACCGGGTGCTGTAGGCTTT376070.23090288202801093No Hit
TGAGGTAGTAGTTTGTACAGTT372810.22890127755168652No Hit
TGTAAACATCCTACACTCTCAGCT361520.22196934057693118No Hit
AACATTCAACGCTGTCGGTGAGTT353110.21680569221929677No Hit
CATTGCACTTGTCTCGGTCTGA351190.21562683314121614No Hit
CTTCTCACTACTGCACTTGACTAG306670.18829203826822163No Hit
CGCGACCTCAGATCAGACGTGG305660.18767190927402297No Hit
CAAAGAATTCTCCTTTTGGGCT304550.1869903813695076No Hit
CCCCCCACTGCTAAATTTGACTGTA303540.18637025237530894No Hit
AGCTCGGTCTGAGGCCCCTCAGT297760.1828213953590037No Hit
TTCAAGTAATCCAGGATAGGCTT286810.17609821467932515No Hit
GCCTGTCTGAGCGTCGCT286300.17578508023671No Hit
CTACGCCTGTCTGAGCGTCGCT271940.16696819671523197No Hit
CTCCCACTGCTTCACTTGACTAGCCT255270.1567329983654382No Hit
GGAATACCGGGTGCTGTAGGCTTT253770.1558120147106877No Hit
TGAGAACTGAATTCCATGGGTT253750.15579973492862437No Hit
TAGCAGCACGTAAATATTGGC246430.15130533469344198No Hit
TGAGGTAGTAGTTTGTGCTGTT245820.15093080134051012No Hit
GTGCGGTAACGCGACCGATCCCGGA241760.14843800558165213No Hit
CCCATAAAGTAGAAAGCACT239860.14727142628563486No Hit
CCCCCCACTGCTAAATTTGACTGT238490.14643026121429606No Hit
TACGCCTGTCTGAGCGTCGCT227820.13987899748350427No Hit
CAAAGAATTCTCCTTTTGGGCTT225120.1382212269049534No Hit
GGAATACCGGGTGCTGTAGGCTT221410.13594332733220382No Hit
CGCCCCACTGCTAAATTTGACTG218930.1344206343563497No Hit
TGATGTAGTAGGTTGTATAGTT215920.13257252715581705No Hit
TGTCAGTTTGTCAAATACCCCA213470.13106825385305793No Hit
TATTGCACTTGTCCCGGCCTGTT212740.13062004180774603No Hit
AACGGAATCCCAAAAGCAGCTG199600.12255222499213173No Hit
TGTAAACATCCCCGACTGGAAGC196830.12085147517635916No Hit
AGCAGCATTGTACAGGGCTATGT195400.11997347075883036No Hit
CCCCCACTGCTAAATTTGACTGGCT194990.11972173522653189No Hit
GGGCTACGCCTGTCTGAGCGTCGC189910.11660267058244357No Hit
ACTGGACTTGGAGTCAGAAGGCA187910.11537469237610957No Hit
GGGAATACCGGGTGCTGTAGGCTT185900.11414057427874393No Hit
CGCGACCTCAGATCAGACGTGGC185170.11369236223343203No Hit
TAGCAGCACGTAAATATTGGCGT184060.11301083432891668No Hit
TGAAAAGAACTTTGAAGAGAG180850.11103992930775063No Hit
TTATAAAGCAATGAGACTGATT178160.1093882986202314No Hit
ACTTGACTTGGAGTCAGAAGGC177380.10890938711976117No Hit
CCCGCCACTGCTAAATTTGACTG176070.10810506139461239No Hit
GACCCGCGGGCGCTCTCCAGTCCT175710.10788402531747227No Hit
CAAAGTGCTGTTCGTGCAGGTAG174250.10698760122684846No Hit
ATCACATTGCCAGGGATTTCCA171260.10515177380837916No Hit
TGTCAGTTTGTCAAATACCCCAA169910.10432288851910372No Hit
ACTGGACTTGGAGTCAGAAGGCT166860.10245022175444439No Hit
TCCCACTGCTTCACTTGACTAG166430.1021862064400826No Hit
CGCGACCTCAGATCAGACGTGGCGAC165160.1014064402790605No Hit
CAACGGAATCCCAAAAGCAGCT163690.10050387629740502No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)