FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947250

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947250
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6712857
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG6548639.755354538313568No Hit
CAACGGAATCCCAAAAGCAGCTG3594485.354620245895302No Hit
CCCCCACTGCTAAATTTGACTG2087213.1092722517402054No Hit
AAGCTGCCAGTTGAAGAACTGT1907612.8417259596025954No Hit
TAGCAGCACGTAAATATTGGCG1899442.82955528473197No Hit
AACATTCAACGCTGTCGGTGAGT1798532.6792318084535394No Hit
TTCAAGTAATCCAGGATAGGCT1512572.253243291194792No Hit
TAGCTTATCAGACTGATGTTGAC1187501.768993440497839No Hit
AACATTCAACGCTGTCGGTGAG902081.3438093497299286No Hit
TAGCTTATCAGACTGATGTTGACT886211.3201681489714439No Hit
TAGCTTATCAGACTGATGTTGA666990.9936007872653924No Hit
TATTGCACTTGTCCCGGCCTGTAA665020.9906661202525244No Hit
TATTGCACTTGTCCCGGCCTGTAT619830.9233475403989687No Hit
TATTGCACTTGTCCCGGCCTGT584010.8699872498401203No Hit
TGTAAACATCCTTGACTGGAAGCT579180.8627921017831901No Hit
AGCAGCATTGTACAGGGCTATGA421250.6275271467871281No Hit
CATAAAGTAGAAAGCACTACT411220.6125856695591758No Hit
ACCGGGTGCTGTAGGCTT377790.562785711061624No Hit
TATTGCACTTGTCCCGGCCTGTA373800.5568418931015512No Hit
TGAGGTAGTAGGTTGTATAGTT362090.5393977556798841No Hit
TGTAAACATCCCCGACTGGAAGCT358570.5341540867025768No Hit
TTAATGCTAATCGTGATAGGGGTT349000.5198978616705227No Hit
ACTGGACTTGGAGTCAGAAGGC310600.46269420009989787No Hit
TTCAAGTAATTCAGGATAGGTT272250.4055650224636098No Hit
CCCATAAAGTAGAAAGCACTAC261850.39007236412156554No Hit
CCCCCACTGCTAAATTTGACTGGCTT261430.38944669907313684No Hit
CAACGGAATCCCAAAAGCAGCTGT254210.3786912189549099No Hit
CGCCTGTCTGAGCGTCGCT240220.357850614127487No Hit
TGAGGTAGTAGATTGTATAGTT200700.29897851242771895No Hit
TAGCTTATCAGACTGATGTTGACA188640.2810129874656946No Hit
ACCGGGTGCTGTAGGCTTT187630.2795084119920922No Hit
TGTAAACATCCTACACTCTCAGCT178890.2664886202700281No Hit
CATTGCACTTGTCTCGGTCTGA176940.2635837468308948No Hit
ACCCCCCACTGCTAAATTTGACTG175880.2620046874229557No Hit
AACATTCAACGCTGTCGGTGAGTT166050.24736114593235042No Hit
TCGTACGACTCTTAGCGG163480.2435326717074414No Hit
GGGCTACGCCTGTCTGAGCGTCGCT153240.22827836195527476No Hit
ACCGGGTGCTGTAGGCTTA142360.21207065784359774No Hit
CCCCCCACTGCTAAATTTGACTGG142160.21177272210625075No Hit
CAAAGAATTCTCCTTTTGGGCT140260.20894233260145423No Hit
ACCTGGTGCTGTAGGCTT132850.1979038135327477No Hit
TGATGTAGTAGGTTGTATAGTT128210.19099170442629718No Hit
CCCATAAAGTAGAAAGCACT124530.1855096868591123No Hit
TGTCAGTTTGTCAAATACCCCAA123200.18352841420575472No Hit
ACTTGACTTGGAGTCAGAAGGC123080.18334965276334653No Hit
CAACGGAATCCCAAAAGCAGCT120770.17990849499698863No Hit
CTACGCCTGTCTGAGCGTCGCT115690.17234092726837472No Hit
CGCGACCTCAGATCAGACGTGGCG114640.17077676464730293No Hit
CCCCCCACTGCTAAATTTGACTGGCT112750.16796127192937374No Hit
CTCCCCACTGCTAAATTTGACTG109570.16322409370555638No Hit
CAAAGAATTCTCCTTTTGGGCTT108240.16124282105219878No Hit
TTCAAGTAATCCAGGATAGGCTT105960.15784635364644292No Hit
TGTCAGTTTGTCAAATACCCCA104480.1556416291900751No Hit
CACTAGATTGTGAGCTCCTGGA104220.15525431273152399No Hit
CTCCCACTGCTTCACTTGACTAG103750.15455416374875855No Hit
CTTCTCACTACTGCACTTGACTAG101270.1508597606056557No Hit
CGCCCCACTGCTAAATTTGACTG101190.1507405863107169No Hit
TATTGCACTTGTCCCGGCCTGTT100740.1500702309016861No Hit
TTCTCACTACTGCACTTGACTAG99930.14886359116543074No Hit
TGTAAACATCCCCGACTGGAAGC98490.14671845385653232No Hit
TAGCAGCACGTAAATATTGGC97390.1450798073011238No Hit
CCCGCCACTGCTAAATTTGACTG97060.14458821333450125No Hit
CACTAGATTGTGAGCTCCTGGAA96830.14424558723655218No Hit
CAACACCAGTCGATGGGCTGT96610.1439178579254705No Hit
TGGCTACGCCTGTCTGAGCGTCGCT96040.14306874107403153No Hit
CCCCCCACTGCTAAATTTGACTGTA90780.1352330311818053No Hit
CCCCCCACTGCTAAATTTGACTGT90750.13518834082120323No Hit
TTAATGCTAATCGTGATAGGGGTTT89750.13369866213446824No Hit
TGAGGTAGTAGTTTGTACAGTT88620.13201532521845766No Hit
TCGTTTCCCGGCCAATGCACCA86830.129348800369202No Hit
AGCAGCATTGTACAGGGCTATGT86750.12922962607426317No Hit
ACTGGACTTGGAGTCAGAAGGCA86670.12911045177932434No Hit
TACGCCTGTCTGAGCGTCGCT85960.12805277991174252No Hit
CTCCCACTGCTTCACTTGACTAGCCT85360.1271589726997015No Hit
TATTGCACTTGTCCCGGCCTGTAG83810.12484997073526219No Hit
TAGCAGCACGTAAATATTGGCGT83060.12373271172021093No Hit
GTGCGGTAACGCGACCGATCCCGGA82360.12268993663949641No Hit
TGAAAAGAACTTTGAAGAGAG82220.12248138162335351No Hit
TTATAAAGCAATGAGACTGATT81620.1215875744113125No Hit
ATCACATTGCCAGGGATTTCCA78520.11696957048243393No Hit
CGCGACCTCAGATCAGACGTGG77220.1150329881896784No Hit
TGTAAACATCCTACACTCAGCT77120.11488402032100489No Hit
TGCAAGTAATCCAGGATAGGCT75540.11253032799596356No Hit
ACCACAGGGTAGAACCACGGAC75380.11229197940608596No Hit
TGAGGTAGTAGTTTGTGCTGTT73750.10986380314670788No Hit
AGCTCGGTCTGAGGCCCCTCAGT72290.10768887226407474No Hit
TGATGTAGTAGATTGTATAGTT71950.10718238151058485No Hit
TTTAAACATCCTTGACTGGAAGCT68780.10246010007363482No Hit
ACGCCTGTCTGAGCGTCGCT68460.10198340289387961No Hit
TTCAAGTAATCCAGGATAGGC68200.10159608643532851No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)