FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947252

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947252
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6809488
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG74753610.977859128322129No Hit
CAACGGAATCCCAAAAGCAGCTG5322697.816578867603556No Hit
AACATTCAACGCTGTCGGTGAGT2595053.810932628121233No Hit
TTCAAGTAATCCAGGATAGGCT2564803.7665093175874604No Hit
CCCCCACTGCTAAATTTGACTG2271383.33561054810582No Hit
TAGCAGCACGTAAATATTGGCG2182963.2057623128199944No Hit
AAGCTGCCAGTTGAAGAACTGT2054773.017510273900182No Hit
AACATTCAACGCTGTCGGTGAG1513502.222634065879843No Hit
TATTGCACTTGTCCCGGCCTGTAA1416662.080420730604122No Hit
TATTGCACTTGTCCCGGCCTGTAT1340771.9689732913840217No Hit
TAGCTTATCAGACTGATGTTGACT1290131.894606466741699No Hit
TAGCTTATCAGACTGATGTTGAC1133391.6644276339131518No Hit
TAGCTTATCAGACTGATGTTGA1001211.4703161236204543No Hit
TGAGGTAGTAGGTTGTATAGTT935241.3734365931770494No Hit
TGAGGTAGTAGATTGTATAGTT754341.1077778534891316No Hit
TGTAAACATCCTTGACTGGAAGCT748591.0993337531397367No Hit
TATTGCACTTGTCCCGGCCTGT631470.9273384430664978No Hit
CATAAAGTAGAAAGCACTACT537000.7886055456739186No Hit
TATTGCACTTGTCCCGGCCTGTA531060.7798824228781958No Hit
AGCAGCATTGTACAGGGCTATGA476000.6990246550107733No Hit
TGTAAACATCCCCGACTGGAAGCT464710.6824448475421354No Hit
TTCAAGTAATTCAGGATAGGTT442670.6500782437681071No Hit
CCCATAAAGTAGAAAGCACTAC431040.6329991329744615No Hit
ACTGGACTTGGAGTCAGAAGGC396520.5823051600942685No Hit
TTCTCACTACTGCACTTGACTAG352160.5171607615726762No Hit
CTTCTCACTACTGCACTTGACTAG338120.4965424713282408No Hit
CAACGGAATCCCAAAAGCAGCTGT331060.4861745846383752No Hit
TCGTACGACTCTTAGCGG300750.44166316175313036No Hit
TGAGGTAGTAGTTTGTACAGTT277470.4074755693820152No Hit
CAACGGAATCCCAAAAGCAGCT255000.37447749375577133No Hit
CCCCCACTGCTAAATTTGACTGGCTT245690.3608053938857077No Hit
CCCATAAAGTAGAAAGCACT244880.3596158771408364No Hit
CTCCCACTGCTTCACTTGACTAG244090.3584557311797891No Hit
CATTGCACTTGTCTCGGTCTGA238230.3498500915193624No Hit
CCCCCCACTGCTAAATTTGACTGGCT220410.32368072313219437No Hit
TGTAAACATCCTACACTCTCAGCT217850.3199212628027247No Hit
CAAAGAATTCTCCTTTTGGGCT193790.28458820986247424No Hit
GGAATACCGGGTGCTGTAGGCTTT180640.26527691949820603No Hit
TGTCAGTTTGTCAAATACCCCAA179630.2637936949150949No Hit
CACCCGGCTGTGTGCACATGTGC178130.2615908861282963No Hit
AACATTCAACGCTGTCGGTGAGTT176990.25991675145032933No Hit
ACCCCCCACTGCTAAATTTGACTG174800.2567006506216033No Hit
TATTGCACTTGTCCCGGCCTGTAG166520.2445411461184747No Hit
CACTAGATTGTGAGCTCCTGGA161110.23659634909408755No Hit
TGAGGTAGTAGTTTGTGCTGTT159160.23373269767124927No Hit
CCCCCCACTGCTAAATTTGACTGG153530.2254648220247983No Hit
TAGCTTATCAGACTGATGTTGACA152430.22384942891447934No Hit
CAACACCAGTCGATGGGCTGT150120.2204571033828094No Hit
CACTAGATTGTGAGCTCCTGGAA149320.2192822720298501No Hit
TTCAAGTAATCCAGGATAGGCTT141560.20788640790614507No Hit
CAAAGAATTCTCCTTTTGGGCTT140130.20578639686273034No Hit
AGCTCGGTCTGAGGCCCCTCAGT132600.1947282967530011No Hit
AACGGAATCCCAAAAGCAGCTG129900.19076324093676353No Hit
TGTAAACATCCCCGACTGGAAGC126610.18593174699771847No Hit
CCCCCCACTGCTAAATTTGACTGT126370.1855792975918307No Hit
TATTGCACTTGTCCCGGCCTGTT126280.18544712906462277No Hit
GGAATACCGGGTGCTGTAGGCTT124920.183449915764592No Hit
CTCCCACTGCTTCACTTGACTAGCCT124760.18321494949400013No Hit
TCGTTTCCCGGCCAATGCACCA124490.1828184439123764No Hit
TTCAAGTAATCCAGGATAGGC120300.17666526470125213No Hit
ACTGGACTTGGAGTCAGAAGGCT117430.1724505572225107No Hit
TAGCAGCACGTAAATATTGGC116560.1711729281261675No Hit
ACTGGACTTGGAGTCAGAAGGCA114110.16757500710772968No Hit
CGCCTGTCTGAGCGTCGCT111710.16405051304885185No Hit
TGTCAGTTTGTCAAATACCCCA111360.16353652433193214No Hit
TATTGCACTTGTCCCGGCCTGTTT109380.16062881673335794No Hit
TATTGCACTTGTCCCGGCCTGTAAT107360.15766236756713572No Hit
TTATAAAGCAATGAGACTGATT104550.15353577243986627No Hit
AGCAGCATTGTACAGGGCTATGT103590.1521259748163151No Hit
TTCACAGTGGCTAAGTTCCG103490.1519791208971952No Hit
AACATTCAACGCTGTCGGTGA100530.14763224489124585No Hit
TTCACAGTGGCTAAGTTCTGC99340.14588468325371892No Hit
CCCCCCACTGCTAAATTTGACTGTA96320.14144969489629763No Hit
TTCACAGTGGCTAAGTTCCGC95210.13981961639406665No Hit
CCCATAAAGTAGAAAGCACTA95000.13951122316391482No Hit
ATGCACCTGGGCAAGGATTCTGA89980.13213915642409532No Hit
TCGTTTCCCGGGCGGCGCACCA84830.12457617958941995No Hit
ACTGGACTTGGAGTCAGAAGG84020.12338666284454868No Hit
TATTGCACTTGTCCCGGCCTGTAAA80830.11870202282462353No Hit
TGAGGTAGTAGGTTGTATAGT80480.11818803410770383No Hit
AACATTCAACGCTGTCGGTGAGA80020.11751250607975226No Hit
TTCACAGTGGCTAAGTTCTG79250.11638173090252894No Hit
CCCCCACTGCTAAATTTGACTGGCT74460.10934742817668523No Hit
TGAGGTAGTAGGTTGTGTGGTT74370.10921525964947731No Hit
TATTGCACTTGTCCCGGCCTGA73050.10727678791709451No Hit
CTACGCCTGTCTGAGCGTCGCT72110.10589636107736734No Hit
CAACGGAATCCCAAAAGCAGCTGA71850.10551454088765558No Hit
AGCAGCATTGTACAGGGCTATG71800.10544111392809562No Hit
TAGCAGCACGTAAATATTGGCGT70630.10372292307439268No Hit
ACCACAGGGTAGAACCACGGAC68610.10075647390817048No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)