FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947254

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947254
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8660860
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG6727957.768223940809574No Hit
CCCCCCACTGCTAAATTTGACTG6159477.111845705853691No Hit
TTCAAGTAATCCAGGATAGGCT4268514.928505945137088No Hit
CCCCCACTGCTAAATTTGACTG3003203.467554030431158No Hit
AAGCTGCCAGTTGAAGAACTGT2909113.3589158582404055No Hit
AACATTCAACGCTGTCGGTGAGT2685353.100558143186704No Hit
TAGCAGCACGTAAATATTGGCG2288462.6423011109751227No Hit
AACATTCAACGCTGTCGGTGAG1928352.226510993134631No Hit
CATAAAGTAGAAAGCACTACT1448011.6719009428624871No Hit
TAGCTTATCAGACTGATGTTGAC1316251.519768244723965No Hit
TGAGGTAGTAGGTTGTATAGTT1278281.4759273328514722No Hit
TAGCTTATCAGACTGATGTTGA1226471.416106483651739No Hit
TAGCTTATCAGACTGATGTTGACT1127231.3015220197532347No Hit
TATTGCACTTGTCCCGGCCTGTAA982321.1342060719143363No Hit
TATTGCACTTGTCCCGGCCTGTAT869831.0043228963405482No Hit
TATTGCACTTGTCCCGGCCTGT785610.907080821073196No Hit
TGAGGTAGTAGATTGTATAGTT770820.8900039949843319No Hit
TGTAAACATCCTTGACTGGAAGCT665300.7681685190616174No Hit
TTCAAGTAATTCAGGATAGGTT662220.7646122902344571No Hit
CCCATAAAGTAGAAAGCACTAC597350.6897121071117649No Hit
CCCATAAAGTAGAAAGCACT539190.6225594225053863No Hit
TATTGCACTTGTCCCGGCCTGTA455730.5261948582473334No Hit
TCGTACGACTCTTAGCGG455720.5261833120498426No Hit
AGCAGCATTGTACAGGGCTATGA444750.5135171334024566No Hit
CATTGCACTTGTCTCGGTCTGA412770.476592393826941No Hit
CAACGGAATCCCAAAAGCAGCT406500.46935292800022166No Hit
CAACGGAATCCCAAAAGCAGCTGT396570.4578875538918768No Hit
CGCCTGTCTGAGCGTCGCT351680.40605667335576373No Hit
TTCTCACTACTGCACTTGACTAG329150.3800430904090356No Hit
CAACACCAGTCGATGGGCTGT325760.3761289294596611No Hit
TGTAAACATCCTACACTCTCAGCT319240.36860080869567224No Hit
TCGTTTCCCGGCCAATGCACCA316600.3655526125581063No Hit
TGAGGTAGTAGTTTGTACAGTT287130.33152596855277655No Hit
CTCCCACTGCTTCACTTGACTAG284060.32798128592310694No Hit
TGTAAACATCCCCGACTGGAAGCT283600.32745016083853107No Hit
ACTGGACTTGGAGTCAGAAGGC271670.31367554723203006No Hit
TTCAAGTAATCCAGGATAGGC243270.28088434635821385No Hit
ACCGGGTGCTGTAGGCTT240540.2777322344432308No Hit
CACCCGGCTGTGTGCACATGTGC239760.27683163103895No Hit
TTCACAGTGGCTAAGTTCCGC234510.27076987735629027No Hit
TGATGTAGTAGGTTGTATAGTT223630.25820761448632124No Hit
TTCACAGTGGCTAAGTTCTGC210980.24360167466048405No Hit
CCCCCACTGCTAAATTTGACTGGCTT194120.2241347856910284No Hit
TTAATGCTAATCGTGATAGGGGTT193160.2230263507319135No Hit
CTTCTCACTACTGCACTTGACTAG186480.2153134908080722No Hit
GCCTGTCTGAGCGTCGCT183980.21242694143537708No Hit
TTCACAGTGGCTAAGTTCCG182470.21068346561426923No Hit
TGTAAACATCCTACACTCAGCT177320.20473717390651738No Hit
AACGGAATCCCAAAAGCAGCTG171210.19768244723965053No Hit
ACCGGGTGCTGTAGGCTTA165160.19069699775772844No Hit
TGAGGTAGTAGTTTGTGCTGTT164710.19017741887064332No Hit
CTACGCCTGTCTGAGCGTCGCT163330.18858404361691564No Hit
TTCAAGTAATCCAGGATAGGCTT159130.18373464067078787No Hit
CAAAGAATTCTCCTTTTGGGCT158060.1824991975392744No Hit
ACCCCCCACTGCTAAATTTGACTG157450.18179487949233678No Hit
ACCGGGTGCTGTAGGCTTT156570.1807788141131481No Hit
CCCATAAAGTAGAAAGCACTA150960.17430139732082034No Hit
AACATTCAACGCTGTCGGTGAGTT150050.17325069334915932No Hit
TAGCAGCACGTAAATATTGGC140800.1625704606701875No Hit
TTATAAAGCAATGAGACTGATT140260.16194696600568534No Hit
TTCACAGTGGCTAAGTTCTG137730.15902577804051793No Hit
AGCTCGGTCTGAGGCCCCTCAGT134930.1557928427430994No Hit
ATCCCGGACGAGCCCCCA133650.15431492946427955No Hit
TGATGTAGTAGATTGTATAGTT131980.1523867144833192No Hit
TAGCTTATCAGACTGATGTTGACA130760.15097807838944402No Hit
TGTCAGTTTGTCAAATACCCCA129930.15001974399770923No Hit
ATCACATTGCCAGGGATTTCC128030.14782596647446097No Hit
CCCCCCACTGCTAAATTTGACTGGCT127690.14743339575977443No Hit
CCCCCCACTGCTAAATTTGACTGG126910.14653279235549357No Hit
TGAGGTAGTAGGTTGTATAGT126850.14646351517054887No Hit
CACTAGATTGTGAGCTCCTGGA125060.14439674581969922No Hit
TCCCACTGCTTCACTTGACTAG123610.14272254718353605No Hit
TGAGGTAGTAGGTTGTGTGGTT122810.14179885138427362No Hit
GGAATACCGGGTGCTGTAGGCTTT122290.14119844911475304No Hit
TCGAGGAGCTCACAGTCTAGT121610.14041330768537996No Hit
CAAAGTGCTGTTCGTGCAGGT118810.13718037238796146No Hit
TATTGCACTTGTCCCGGCCTGTT116970.13505587204965788No Hit
AACCGGGCGGAAACACCA116330.13431691541024796No Hit
TGTCAGTTTGTCAAATACCCCAA111070.12824361553009747No Hit
TAGCAGCACATCATGGTTTACA109850.12683497943622227No Hit
AATGCACCCGGGCAAGGATTCT109640.12659250928891588No Hit
AACATTCAACGCTGTCGGTGA109040.12589973743946906No Hit
CATAAAGTAGAAAGCACTAC107390.1239946148534903No Hit
AGAGGTAGTAGGTTGCATAGTT107370.12397152245850875No Hit
TGTCAGTTTGTCAAATACCCC105250.1215237285904633No Hit
TATTGCACTTGTCCCGGCCTGTAG104040.12012663869407887No Hit
CACCCGTAGAACCGACCTTGCG103510.11951469022706752No Hit
GGAATACCGGGTGCTGTAGGCTT100790.11637412450957525No Hit
CAAAGAATTCTCCTTTTGGGCTT99140.1144690019235965No Hit
AGCAGCATTGTACAGGGCTATGT97140.11215976242544043No Hit
CACTAGATTGTGAGCTCCTGGAA96640.11158245255090142No Hit
ACCACAGGGTAGAACCACGGAC95880.1107049415416021No Hit
ATCCCACCGCTGCCACCA94190.10875363416566021No Hit
AGCAGCATTGTACAGGGCTATG93250.10766829160152687No Hit
TGTCAGTTTGTCAAATACC90820.10486256561126725No Hit
ACGCCTGTCTGAGCGTCGCT90370.10434298672418213No Hit
TACGCCTGTCTGAGCGTCGCT88650.10235704075576792No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)