FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947255

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947255
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8956540
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG97823610.922030158967637No Hit
CAACGGAATCCCAAAAGCAGCTG5814076.491424143698348No Hit
GGAATACCGGGTGCTGTAGGCTTT4643315.1842675854738545No Hit
CCCCCACTGCTAAATTTGACTG3261853.6418639340638235No Hit
GGAATACCGGGTGCTGTAGGCTT3011963.362861104846291No Hit
TTCAAGTAATCCAGGATAGGCT2349202.62288785624806No Hit
TGAATACCGGGTGCTGTAGGCTTT1792022.000794949835539No Hit
AACATTCAACGCTGTCGGTGAGT1754551.9589595982377122No Hit
TAGCTTATCAGACTGATGTTGAC1631561.8216409461689447No Hit
TAGCAGCACGTAAATATTGGCG1568741.751502254218705No Hit
AACATTCAACGCTGTCGGTGAG1536981.7160421323412838No Hit
TAGCTTATCAGACTGATGTTGACT1503081.6781926949469326No Hit
TATTGCACTTGTCCCGGCCTGTAA1443061.6111802102151054No Hit
AAGCTGCCAGTTGAAGAACTGT1373681.5337172613531564No Hit
TATTGCACTTGTCCCGGCCTGTAT1301751.4534072309172963No Hit
TGAATACCGGGTGCTGTAGGCTT1136981.2694411011395024No Hit
TAGCTTATCAGACTGATGTTGA1108591.237743592949956No Hit
GGAATACCGGGTGCTGTAGGCTTA849620.9486029203241431No Hit
TGTAAACATCCTTGACTGGAAGCT806010.8999122428973688No Hit
TGAGGTAGTAGGTTGTATAGTT734280.8198255129771095No Hit
TTCAAGTAATTCAGGATAGGTT641840.7166160146663779No Hit
TCGTACGACTCTTAGCGG640860.7155218421399335No Hit
TGAGGTAGTAGATTGTATAGTT597470.6670767952803203No Hit
TATTGCACTTGTCCCGGCCTGTA590820.6596520531365907No Hit
TATTGCACTTGTCCCGGCCTGT565710.631616673402899No Hit
AGCAGCATTGTACAGGGCTATGA472270.5272906725141628No Hit
CATAAAGTAGAAAGCACTACT469730.5244547559660315No Hit
GGAATACCGGGTGCTGTAGGCT436230.48705191960288235No Hit
TGTAAACATCCCCGACTGGAAGCT417430.4660616711363986No Hit
CCCATAAAGTAGAAAGCACTAC362440.4046651943719338No Hit
CCCCCACTGCTAAATTTGACTGGCTT336640.37585942785941895No Hit
TTAATGCTAATCGTGATAGGGGTT334890.3739055483479111No Hit
TGAATACCGGGTGCTGTAGGCTTA333290.372119144223104No Hit
TTCTCACTACTGCACTTGACTAG331140.36971866368039447No Hit
CAACGGAATCCCAAAAGCAGCTGT303980.3393944536617935No Hit
CAAAGAATTCTCCTTTTGGGCT285140.3183595450921896No Hit
CTTCTCACTACTGCACTTGACTAG281720.3145411062754144No Hit
TGTAAACATCCTACACTCTCAGCT277000.30927121410723335No Hit
CATTGCACTTGTCTCGGTCTGA274590.30658044289424263No Hit
CAACGGAATCCCAAAAGCAGCT249070.278087297103569No Hit
TAGCTTATCAGACTGATGTTGACA246880.27564215645773926No Hit
ACTGGACTTGGAGTCAGAAGGC246010.2746707992148754No Hit
CCCATAAAGTAGAAAGCACT243000.271310126455082No Hit
ACCCCCCACTGCTAAATTTGACTG241980.2701712938255174No Hit
TGAGGTAGTAGTTTGTACAGTT215230.24030484986389836No Hit
CTCCCACTGCTTCACTTGACTAG207960.23218787612180597No Hit
TATTGCACTTGTCCCGGCCTGTAG183730.20513501865675807No Hit
CACTAGATTGTGAGCTCCTGGAA180340.20135007491732296No Hit
CCCCCCACTGCTAAATTTGACTGGCT177240.1978889169255092No Hit
TGAATACCGGGTGCTGTAGGCT166920.18636661032050322No Hit
CCCCCCACTGCTAAATTTGACTGG165570.18485933184019723No Hit
TCGTTTCCCGGCCAATGCACCA157890.176284592041123No Hit
CACCCGGCTGTGTGCACATGTGC155550.1736719760085926No Hit
CACTAGATTGTGAGCTCCTGGA154900.1729462493328897No Hit
CCCCCCACTGCTAAATTTGACTGT152310.1700545076558582No Hit
TGTCAGTTTGTCAAATACCCCA147750.16496325590015787No Hit
GGAATACCGGGTGCTGTAGGCTA143230.15991666424757775No Hit
TGATGTAGTAGGTTGTATAGTT141620.15811909509699057No Hit
CGAATACCGGGTGCTGTAGGCTTT140210.1565448264620043No Hit
AACATTCAACGCTGTCGGTGAGTT139980.15628803086906326No Hit
TGTCAGTTTGTCAAATACCCCAA139800.1560870604050225No Hit
CAAAGAATTCTCCTTTTGGGCTT133120.14862882318395274No Hit
TATTGCACTTGTCCCGGCCTGTT132700.14815989210119088No Hit
CCCCCCACTGCTAAATTTGACTGTA127740.14262203931428877No Hit
TAGCAGCACGTAAATATTGGC125400.14000942328175836No Hit
AACGGAATCCCAAAAGCAGCTG124040.1384909797756723No Hit
TTCAAGTAATCCAGGATAGGCTT120380.13440458034017602No Hit
TGAGGTAGTAGTTTGTGCTGTT119550.1334778832004323No Hit
TATTGCACTTGTCCCGGCCTGTAAT115720.12920167832667526No Hit
TTCAAGTAATCCAGGATAGGC114800.12817449595491115No Hit
TGATGTAGTAGATTGTATAGTT114340.1276609047690291No Hit
AGCTCGGTCTGAGGCCCCTCAGT113980.1272589638409475No Hit
CCCATAAAGTAGAAAGCACTA112720.12585217059266188No Hit
CAACACCAGTCGATGGGCTGT111250.12421091180299536No Hit
TATTGCACTTGTCCCGGCCTGTAAA111040.12397644626161441No Hit
TTCACAGTGGCTAAGTTCCG111040.12397644626161441No Hit
ATGCACCTGGGCAAGGATTCTGA105100.11734442094826797No Hit
TATTGCACTTGTCCCGGCCTGTTT104950.1171769455615673No Hit
CTCCCACTGCTTCACTTGACTAGCCT104710.11690898494284624No Hit
TCGTTTCCCGGGCGGCGCACCA102010.11389442798223422No Hit
TTATAAAGCAATGAGACTGATT101980.11386093290489407No Hit
AGCAGCATTGTACAGGGCTATGT99240.11080171584116189No Hit
TGTAAACATCCCCGACTGGAAGC98860.1103774448615202No Hit
GCCTGTCTGAGCGTCGCT97350.10869152596873347No Hit
ATCACATTGCCAGGGATTTCC94710.1057439591628017No Hit
CGAATACCGGGTGCTGTAGGCTT93220.10408037032157506No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)